RESUMO
We report the draft genomes of seven bacterial strains (six Pseudomonas spp. and one Rheinheimera sp.) isolated from environmental water samples from oil sands tailings ponds that have accumulated a wide variety of organic compounds, salts and metals.
RESUMO
IMPORTANCE: New approaches are needed to discover novel antimicrobials, particularly antibiotics that target the Gram-negative outer membrane. By exploiting bacterial sensing and responses to outer membrane (OM) damage, we used a biosensor approach consisting of polymyxin resistance gene transcriptional reporters to screen natural products and a small drug library for biosensor activity that indicates damage to the OM. The diverse antimicrobial compounds that cause induction of the polymyxin resistance genes, which correlates with outer membrane damage, suggest that these LPS and surface modifications also function in short-term repair to sublethal exposure and are required against broad membrane stress conditions.
Assuntos
Plantas Medicinais , Infecções por Pseudomonas , Pseudomonas aeruginosa , Antibacterianos/farmacologia , PolimixinasRESUMO
Although bacterial isolates from Cannabis flowers were reported and sequenced, few from its rhizosphere have been characterized. Here we report the draft genomes of six bacterial strains isolated from Cannabis rhizosphere soil samples. These sequences may shed light on plant-microbe interactions in the Cannabis rhizosphere at the molecular level.