RESUMO
A Western blot method (WB) was adapted for rapid screening of antibodies against coxsackie virus B1-B6 in sera from patients with newly diagnosed insulin-dependent diabetes mellitus, myocarditis or febrile syndrome of suspected coxsackie viral aetiology. The use of a mixture of all 6 coxsackie virus B serotypes as the common antigen permitted a very rapid and inexpensive detection of antibody-positive sera for preliminary diagnosis and further detailed assay. Comparison of the results with those obtained in parallel run virus-neutralization tests showed a higher sensitivity and comparable specificity of WB.
Assuntos
Anticorpos Antivirais/sangue , Infecções por Coxsackievirus/virologia , Enterovirus Humano B/imunologia , Antígenos Virais/imunologia , Western Blotting , Infecções por Coxsackievirus/sangue , Humanos , Testes de Neutralização , Sensibilidade e EspecificidadeRESUMO
Cytokines have been suggested to play an important role in the pathogenesis of type I diabetes mellitus through their direct and indirect effects on the pancreatic islet cells. We studied the time course of tumour necrosis factor alpha (TNF-alpha) and glucose levels in the sera of mice infected with coxsackie B4 and A7 viruses. Two correlating peaks of TNF-alpha and glucose were found. These results suggest the involvement of TNF-alpha in the damage of the insulin producing cells and thus an immunity-related inflammatory process.
Assuntos
Glicemia/análise , Enterovirus Humano B , Fator de Necrose Tumoral alfa/análise , Animais , Diabetes Mellitus Tipo 1/etiologia , Masculino , Camundongos , Organismos Livres de Patógenos Específicos , Fatores de TempoRESUMO
We have demonstrated the ability of 4 standard coxsackie viruses (B4, B5, A7, and A9) and one fresh isolate (A7) from a newly diabetic child with homologous serological response, to infect in vitro grown mouse pancreatic islet cells. Up to the 9th day after infection the multiplication of viruses in the cells was proved using virus titration and immunofluorescence test. Isolated pancreatic cells proved to be a suitable model for detailed studies of experimental infection of pancreatic cells with coxsackie viruses.