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1.
Appl Environ Microbiol ; 87(16): e0058021, 2021 07 27.
Artigo em Inglês | MEDLINE | ID: mdl-34085864

RESUMO

Since 1978, the New York State Department of Health's public health laboratory, Wadsworth Center (WC), in collaboration with epidemiology and environmental partners, has been committed to providing comprehensive public health testing for Legionella in New York. Statewide, clinical case counts have been increasing over time, with the highest numbers identified in 2017 and 2018 (1,022 and 1,426, respectively). Over the course of more than 40 years, the WC Legionella testing program has continuously implemented improved testing methods. The methods utilized have transitioned from solely culture-based methods for organism recovery to development of a suite of reference testing services, including identification and characterization by PCR and pulsed-field gel electrophoresis (PFGE). In the last decade, whole-genome sequencing (WGS) has further refined the ability to link outbreak strains between clinical specimens and environmental samples. Here, we review Legionnaires' disease outbreak investigations during this time period, including comprehensive testing of both clinical and environmental samples. Between 1978 and 2017, 60 outbreaks involving clinical and environmental isolates with matching PFGE patterns were detected in 49 facilities from the 157 investigations at 146 facilities. However, 97 investigations were not solved due to the lack of clinical or environmental isolates or PFGE matches. We found 69% of patient specimens from New York State (NYS) were outbreak associated, a much higher rate than observed in other published reports. The consistent application of new cutting-edge technologies and environmental regulations has resulted in successful investigations resulting in remediation efforts. IMPORTANCE Legionella, the causative agent of Legionnaires' disease (LD), can cause severe respiratory illness. In 2018, there were nearly 10,000 cases of LD reported in the United States (https://www.cdc.gov/legionella/fastfacts.html; https://wonder.cdc.gov/nndss/static/2018/annual/2018-table2h.html), with actual incidence believed to be much higher. About 10% of patients with LD will die, and as high as 90% of patients diagnosed will be hospitalized. As Legionella is spread predominantly through engineered building water systems, identifying sources of outbreaks by assessing environmental sources is key to preventing further cases LD.


Assuntos
Legionella/isolamento & purificação , Doença dos Legionários/microbiologia , Surtos de Doenças , Água Doce/microbiologia , Humanos , Legionella/classificação , Legionella/genética , Doença dos Legionários/diagnóstico , Doença dos Legionários/epidemiologia , New York/epidemiologia , Abastecimento de Água
2.
Infect Genet Evol ; 65: 200-209, 2018 11.
Artigo em Inglês | MEDLINE | ID: mdl-30075256

RESUMO

We investigated the value of whole-genome sequencing (WGS) and single nucleotide polymorphism (SNP) analyses in determining the relationships among and evolutionary rates of Legionella species with long-term persistence in three healthcare facilities. We examined retrospective clinical and environmental isolates of Legionella micdadei and Legionella pneumophila serogroup 1 isolates with identical PFGE DNA fingerprints sampled over the course of up to 18 years. WGS analyses demonstrated that heterogeneous populations of Legionella were present within each facility despite displaying the same PFGE profiles. Additionally, clustering of some clinical isolates with those from a separate but related institution exposed a source of infection not previously detected, underscoring the importance of considering phylogenetic relationships when assessing epidemiological links. The data supported an average substitution rate of 0.80 SNPs per genome per year for L. micdadei but a reliable estimate for L. pneumophila serogroup 1 could not be obtained due to complicating factors such as non-chronological links among isolates and inadequate sampling depths. While the substitution rate for L. micdadei is consistent with previous estimates for L. pneumophila, the lack of a temporal signal in our sequence data for L. pneuomphila serogroup 1 isolates suggests either insufficient change to provide an estimate or variable evolutionary rates, which could reflect the presence of both actively dividing and viable but non-culturable Legionella spp. in the built environment. This study highlights the increased discriminatory power of WGS SNP analysis as compared to PFGE, emphasizes the need for extended sampling, and provides insight into the evolution of Legionella from longitudinal investigations.


Assuntos
Legionella/genética , Taxa de Mutação , Filogenia , Eletroforese em Gel de Campo Pulsado , Instalações de Saúde , Humanos , Legionella pneumophila/genética , Legionelose/microbiologia , Polimorfismo de Nucleotídeo Único , Sequenciamento Completo do Genoma
3.
Diagn Microbiol Infect Dis ; 85(4): 416-8, 2016 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-27220605

RESUMO

We examined the use of pulsed-field gel electrophoresis (PFGE) to predict serotype for Salmonella isolates. Between 2012 and 2014 we assessed 4481 isolates, resulting in >90% assigned serotypes. PFGE is efficient for determining serotype in the majority of cases and results in expedited serotype determination, as well as cost savings.


Assuntos
Técnicas Bacteriológicas/métodos , Eletroforese em Gel de Campo Pulsado/métodos , Salmonella/classificação , Salmonella/genética , Sorotipagem/métodos , Técnicas Bacteriológicas/economia , Eletroforese em Gel de Campo Pulsado/economia , Humanos
4.
Appl Environ Microbiol ; 82(12): 3582-3590, 2016 06 15.
Artigo em Inglês | MEDLINE | ID: mdl-27060122

RESUMO

UNLABELLED: A total of 30 Legionella pneumophila serogroup 1 isolates representing 10 separate legionellosis laboratory investigations ("outbreaks") that occurred in New York State between 2004 and 2012 were selected for evaluation of whole-genome sequencing (WGS) approaches for molecular subtyping of this organism. Clinical and environmental isolates were available for each outbreak and were initially examined by pulsed-field gel electrophoresis (PFGE). Sequence-based typing alleles were extracted from WGS data yielding complete sequence types (ST) for isolates representing 8 out of the 10 outbreaks evaluated in this study. Isolates from separate outbreaks sharing the same ST also contained the fewest differences in core genome single nucleotide polymorphisms (SNPs) and the greatest proportion of identical allele sequences in a whole-genome multilocus sequence typing (wgMLST) scheme. Both core SNP and wgMLST analyses distinguished isolates from separate outbreaks, including those from two outbreaks sharing indistinguishable PFGE profiles. Isolates from a hospital-associated outbreak spanning multiple years shared indistinguishable PFGE profiles but displayed differences in their genome sequences, suggesting the presence of multiple environmental sources. Finally, the rtx gene demonstrated differences in the repeat region sequence among ST1 isolates from different outbreaks, suggesting that variation in this gene may be useful for targeted molecular subtyping approaches for L. pneumophila This study demonstrates the utility of various genome sequence analysis approaches for L. pneumophila for environmental source attribution studies while furthering the understanding of Legionella ecology. IMPORTANCE: We demonstrate that whole-genome sequencing helps to improve resolution of Legionella pneumophila isolated during laboratory investigations of legionellosis compared to traditional subtyping methods. These data can be important in confirming the environmental sources of legionellosis outbreaks. Moreover, we evaluated various methods to analyze genome sequence data to help resolve outbreak-related isolates.


Assuntos
Surtos de Doenças , Genótipo , Legionella pneumophila/classificação , Doença dos Legionários/epidemiologia , Doença dos Legionários/microbiologia , Tipagem Molecular/métodos , Sorogrupo , Genoma Bacteriano , Genômica/métodos , Humanos , Legionella pneumophila/genética , Legionella pneumophila/isolamento & purificação , Epidemiologia Molecular/métodos , New York/epidemiologia
5.
J Infect Dis ; 213(4): 502-8, 2016 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-25995194

RESUMO

BACKGROUND: Using a novel combination of whole-genome sequencing (WGS) analysis and geographic metadata, we traced the origins of Salmonella Bareilly isolates collected in 2012 during a widespread food-borne outbreak in the United States associated with scraped tuna imported from India. METHODS: Using next-generation sequencing, we sequenced the complete genome of 100 Salmonella Bareilly isolates obtained from patients who consumed contaminated product, from natural sources, and from unrelated historically and geographically disparate foods. Pathogen genomes were linked to geography by projecting the phylogeny on a virtual globe and produced a transmission network. RESULTS: Phylogenetic analysis of WGS data revealed a common origin for outbreak strains, indicating that patients in Maryland and New York were infected from sources originating at a facility in India. CONCLUSIONS: These data represent the first report fully integrating WGS analysis with geographic mapping and a novel use of transmission networks. Results showed that WGS vastly improves our ability to delimit the scope and source of bacterial food-borne contamination events. Furthermore, these findings reinforce the extraordinary utility that WGS brings to global outbreak investigation as a greatly enhanced approach to protecting the human food supply chain as well as public health in general.


Assuntos
Surtos de Doenças , Doenças Transmitidas por Alimentos/epidemiologia , Infecções por Salmonella/epidemiologia , Salmonella enterica/classificação , Salmonella enterica/isolamento & purificação , Animais , Doenças Transmitidas por Alimentos/microbiologia , Genoma Bacteriano , Genótipo , Humanos , Índia , Epidemiologia Molecular , Tipagem Molecular , Filogeografia , Infecções por Salmonella/microbiologia , Salmonella enterica/genética , Análise de Sequência de DNA , Atum/microbiologia , Estados Unidos/epidemiologia
6.
Am J Infect Control ; 43(9): 971-6, 2015 09 01.
Artigo em Inglês | MEDLINE | ID: mdl-26149750

RESUMO

BACKGROUND: There are multiple treatment options for the control of legionellae in premise hot water systems. Water chemistry plays a role in the efficacy of these treatments and should be considered when selecting a treatment. This study demonstrated the efficacy of copper-silver ionization (CSI) under alkaline water conditions in 2 health care facilities. METHODS: Monitoring for copper (Cu) and silver (Ag) ions was performed, and the corresponding percentage of positive Legionella cultures was monitored. Low Legionella colony forming units (CFU), with a mean <10 CFU/100 mL, and ≤30% positive culture for each sampling period, along with no recurrent disease, were considered indicative of control. RESULTS: CSI treatment was shown to reduce both the number of CFU found and the percentage of samples found to be culture positive. After treatment was established, culture positivity was, for example, reduced from 70% (>10(3) CFU/100 mL) to consistently <30% (38 CFU/100 mL). CONCLUSION: Control of legionellae in premise water systems may be a complex process requiring long-term assessments for adequate control. This work found that CSI could be successful in controlling Legionella under alkaline water conditions, and the evidence suggests that Ag ions are responsible for the control of Legionella pneumophila 1, L pneumophila 6, and L anisa.


Assuntos
Cobre/farmacologia , Infecção Hospitalar/prevenção & controle , Legionella/efeitos dos fármacos , Legionelose/prevenção & controle , Prata/farmacologia , Microbiologia da Água , Contagem de Colônia Microbiana , Desinfecção/métodos , Instalações de Saúde , Humanos , Controle de Infecções/métodos , Legionella pneumophila/efeitos dos fármacos , Legionelose/microbiologia , Doença dos Legionários/microbiologia , Doença dos Legionários/prevenção & controle , Abastecimento de Água/normas
7.
Anaerobe ; 30: 178-180, 2014 12.
Artigo em Inglês | MEDLINE | ID: mdl-25463969

RESUMO

Type F botulism occurs rarely in clinical cases. Two cases of type F botulism in elderly patients that were clustered in time and space are described. Clostridium baratii producing type F botulinum neurotoxin was isolated from both patients; molecular typing of these isolates revealed that they were unrelated strains.

8.
Emerg Infect Dis ; 20(8): 1306-14, 2014 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-25062035

RESUMO

For Salmonella enterica serovar Enteritidis, 85% of isolates can be classified into 5 pulsed-field gel electrophoresis (PFGE) types. However, PFGE has limited discriminatory power for outbreak detection. Although whole-genome sequencing has been found to improve discrimination of outbreak clusters, whether this procedure can be used in real-time in a public health laboratory is not known. Therefore, we conducted a retrospective and prospective analysis. The retrospective study investigated isolates from 1 confirmed outbreak. Additional cases could be attributed to the outbreak strain on the basis of whole-genome data. The prospective study included 58 isolates obtained in 2012, including isolates from 1 epidemiologically defined outbreak. Whole-genome sequencing identified additional isolates that could be attributed to the outbreak, but which differed from the outbreak-associated PFGE type. Additional putative outbreak clusters were detected in the retrospective and prospective analyses. This study demonstrates the practicality of implementing this approach for outbreak surveillance in a state public health laboratory.


Assuntos
Genoma Bacteriano , Vigilância da População , Infecções por Salmonella/epidemiologia , Infecções por Salmonella/microbiologia , Salmonella enteritidis/genética , Eletroforese em Gel de Campo Pulsado , Genótipo , Humanos , Filogenia , Polimorfismo de Nucleotídeo Único , Estudos Prospectivos , Estudos Retrospectivos , Salmonella enteritidis/classificação , Salmonella enteritidis/isolamento & purificação , Análise de Sequência de DNA
9.
Foodborne Pathog Dis ; 9(11): 1028-36, 2012 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-23009170

RESUMO

Non-O157 Shiga toxin-producing Escherichia coli (STEC) are emerging pathogens with the potential to cause serious illness and impact public health due to diagnostic challenges. Between 2005 and 2010, the Wadsworth Center (WC), the public health laboratory of the New York State (NYS) Department of Health, requested that Shiga toxin enzyme immunoassay (EIA)-positive stool enrichment broths and/or stool specimens be submitted by clinical and commercial reference laboratories testing NYS patient specimens. A total of 798 EIA-positive specimens were received for confirmation and serotyping, and additionally a subset of STEC was assessed for the presence of six virulence genes (stx1, stx2, eaeA, hlyA, nleA, and nleB) by real-time polymerase chain reaction. We confirmed 591 specimens as STEC, 164 (28%) as O157 STEC, and 427 (72%) as non-O157 STEC. Of the non-O157 STEC serogroups identified, over 70% were O103, O26, O111, O45, O121, or O145. During this time period, WC identified and characterized a total of 1282 STEC received as E. coli isolates, stool specimens, or EIA broths. Overall, the STEC testing identified 59% as O157 STEC and 41% as non-O157 STEC; however, out of 600 isolates submitted to the WC as E. coli cultures, 543 (90%) were identified as O157 STEC. This report summarizes a 6-year study utilizing enhanced STEC testing that resulted in increased identification and characterization of non-O157 STEC in NYS. Continued utilization of enhanced STEC testing may lead to effective and timely outbreak response and improve monitoring of trends in STEC disease epidemiology.


Assuntos
Infecções por Escherichia coli/microbiologia , Proteínas de Escherichia coli/genética , Toxina Shiga/genética , Escherichia coli Shiga Toxigênica/isolamento & purificação , Algoritmos , DNA Bacteriano/genética , Infecções por Escherichia coli/embriologia , Fezes/microbiologia , Humanos , Técnicas Imunoenzimáticas , New York/epidemiologia , Saúde Pública , Reação em Cadeia da Polimerase em Tempo Real , Estudos Retrospectivos , Sorotipagem , Escherichia coli Shiga Toxigênica/classificação , Escherichia coli Shiga Toxigênica/genética , Escherichia coli Shiga Toxigênica/imunologia , Fatores de Virulência/genética
10.
Appl Environ Microbiol ; 77(24): 8648-55, 2011 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-22003026

RESUMO

In this study, we report a whole-genome single nucleotide polymorphism (SNP)-based evolutionary approach to study the epidemiology of a multistate outbreak of Salmonella enterica subsp. enterica serovar Montevideo. This outbreak included 272 cases that occurred in 44 states between July 2009 and April 2010. A case-control study linked the consumption of salami made with contaminated black and red pepper to the outbreak. We sequenced, on the SOLiD System, 47 isolates with XbaI PFGE pattern JIXX01.0011, a common pulsed-field gel electrophoresis (PFGE) pattern associated with isolates from the outbreak. These isolates represented 20 isolates collected from human sources during the period of the outbreak and 27 control isolates collected from human, food, animal, and environmental sources before the outbreak. Based on 253 high-confidence SNPs, we were able to reconstruct a tip-dated molecular clock phylogeny of the isolates and to assign four human isolates to the actual outbreak. We developed an SNP typing assay to rapidly discriminate between outbreak-related cases and non-outbreak-related cases and tested this assay on an extended panel of 112 isolates. These results suggest that only a very small percentage of the human isolates with the outbreak PFGE pattern and obtained during the outbreak period could be attributed to the actual pepper-related outbreak (20%), while the majority (80%) of the putative cases represented background cases. This study demonstrates that next-generation-based SNP typing provides the resolution and accuracy needed for outbreak investigations of food-borne pathogens that cannot be distinguished by currently used subtyping methods.


Assuntos
Surtos de Doenças , Doenças Transmitidas por Alimentos/epidemiologia , Tipagem Molecular/métodos , Polimorfismo de Nucleotídeo Único , Infecções por Salmonella/epidemiologia , Salmonella enterica/classificação , Salmonella enterica/genética , Análise por Conglomerados , Eletroforese em Gel de Campo Pulsado , Doenças Transmitidas por Alimentos/microbiologia , Genótipo , Humanos , Epidemiologia Molecular/métodos , Infecções por Salmonella/microbiologia , Salmonella enterica/isolamento & purificação
11.
Int J Syst Evol Microbiol ; 61(Pt 1): 91-98, 2011 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-20173010

RESUMO

An analysis of 16S rRNA gene sequences from archived clinical reference specimens has identified two novel Neisseria species. For each species, two strains from independent sources were identified. Amongst species with validly published names, the closest species to the newly identified organisms were Neisseria canis, N. dentiae, N. zoodegmatis, N. animaloris and N. weaveri. DNA-DNA hybridization studies demonstrated that the newly identified isolates represent species that are distinct from these nearest neighbours. Analysis of partial 23S rRNA gene sequences for the newly identified strains and their nearest neighbours provided additional support for the species designation. Bayesian analysis of 16S rRNA gene sequences suggested that the newly identified isolates belong to distinct but related species of the genus Neisseria, and are members of a clade that includes N. dentiae, N. bacilliformis and N. canis. The predominant cellular fatty acids [16 : 0, summed feature 3 (16 : 1ω7c and/or iso-15 : 0 2-OH) and 18 : 1ω7c], as well as biochemical and morphological analyses further support the designation of Neisseria wadsworthii sp. nov. (type strain 9715(T) =DSM 22247(T) =CIP 109934(T)) and Neisseria shayeganii sp. nov. (type strain 871(T) =DSM 22246(T) =CIP 109933(T)).


Assuntos
Neisseria/classificação , Neisseria/isolamento & purificação , Infecções por Neisseriaceae/microbiologia , Técnicas de Tipagem Bacteriana , Análise por Conglomerados , DNA Bacteriano/química , DNA Bacteriano/genética , DNA Ribossômico/química , DNA Ribossômico/genética , Ácidos Graxos/análise , Humanos , Dados de Sequência Molecular , Neisseria/química , Neisseria/genética , Hibridização de Ácido Nucleico , Filogenia , RNA Ribossômico 16S/genética , RNA Ribossômico 23S/genética , Análise de Sequência de DNA
12.
PLoS One ; 5(5): e10783, 2010 May 24.
Artigo em Inglês | MEDLINE | ID: mdl-20520731

RESUMO

BACKGROUND: Massive die-offs of little brown bats (Myotis lucifugus) have been occurring since 2006 in hibernation sites around Albany, New York, and this problem has spread to other States in the Northeastern United States. White cottony fungal growth is seen on the snouts of affected animals, a prominent sign of White Nose Syndrome (WNS). A previous report described the involvement of the fungus Geomyces destructans in WNS, but an identical fungus was recently isolated in France from a bat that was evidently healthy. The fungus has been recovered sparsely despite plentiful availability of afflicted animals. METHODOLOGY/PRINCIPAL FINDINGS: We have investigated 100 bat and environmental samples from eight affected sites in 2008. Our findings provide strong evidence for an etiologic role of G. destructans in bat WNS. (i) Direct smears from bat snouts, Periodic Acid Schiff-stained tissue sections from infected tissues, and scanning electron micrographs of bat tissues all showed fungal structures similar to those of G. destructans. (ii) G. destructans DNA was directly amplified from infected bat tissues, (iii) Isolations of G. destructans in cultures from infected bat tissues showed 100% DNA match with the fungus present in positive tissue samples. (iv) RAPD patterns for all G. destructans cultures isolated from two sites were indistinguishable. (v) The fungal isolates showed psychrophilic growth. (vi) We identified in vitro proteolytic activities suggestive of known fungal pathogenic traits in G. destructans. CONCLUSIONS/SIGNIFICANCE: Further studies are needed to understand whether G. destructans WNS is a symptom or a trigger for bat mass mortality. The availability of well-characterized G. destructans strains should promote an understanding of bat-fungus relationships, and should aid in the screening of biological and chemical control agents.


Assuntos
Ascomicetos/genética , Quirópteros/microbiologia , Micoses/veterinária , Animais , Ascomicetos/classificação , Ascomicetos/isolamento & purificação , Ascomicetos/ultraestrutura , DNA Fúngico/genética , Técnicas de Tipagem Micológica , Micoses/microbiologia , Micoses/patologia , New York , Especificidade de Órgãos , Filogenia , Técnica de Amplificação ao Acaso de DNA Polimórfico , Análise de Sequência de DNA , Síndrome
13.
Mycopathologia ; 170(4): 279-85, 2010 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-20306144

RESUMO

Cryptococcus neoformans and Cryptococcus gattii are found in distinct environments with some overlap around different parts of the world. However, no systematic surveys of these two pathogens have been reported from Puerto Rico, a tropical island uniquely situated between mainland USA and countries in South America. We carried out an exhaustive environmental survey in southwestern Puerto Rico for pathogenic Cryptococcus species. Twenty-two presumptive isolates of C. gattii from cacti and tree detritus were characterized in detail by physiological and molecular methods and seventeen strains were confirmed as C. gattii. Cryptococcus gattii isolates were haploid and majority of them were MATa [corrected] strains. Sixteen out of seventeen C. gattii isolates belonged to VGII/AFLP6 genotype while one isolate was a VGIV/AFLP7 genotype. The results are significant as Puerto Rico strains are distinct from VGIII/AFLP5 strains reported from Southern California, but similar to C. gattii VGII/AFLP6 molecular type implicated in recent outbreaks of cryptococcosis in Pacific Northwest and British Columbia, Canada, but different in its M13 fingerprinting, and a common genotype in South America.


Assuntos
Cactaceae/microbiologia , Cryptococcus gattii/classificação , Cryptococcus gattii/isolamento & purificação , Microbiologia Ambiental , Análise do Polimorfismo de Comprimento de Fragmentos Amplificados , Análise por Conglomerados , Cryptococcus gattii/genética , Impressões Digitais de DNA , Genótipo , Dados de Sequência Molecular , Porto Rico , Análise de Sequência de DNA , Clima Tropical
14.
J Clin Microbiol ; 47(7): 2142-8, 2009 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-19403775

RESUMO

Our laboratory has developed testing methods that use real-time PCR and pyrosequencing analysis to enable the rapid identification of potential hypervirulent Clostridium difficile strains. We describe a real-time PCR assay that detects four C. difficile genes encoding toxins A (tcdA) and B (tcdB) and the binary toxin genes (cdtA and cdtB), as well as a pyrosequencing assay that detects common deletions in the tcdC gene in less than 4 h. A subset of historical and recent C. difficile isolates (n = 31) was also analyzed by pulsed-field gel electrophoresis to determine the circulating North American pulsed-field (NAP) types that have been isolated in New York State. Thirteen different NAP types were found among the 31 isolates tested, 13 of which were NAP type 1 strains. To further assess the best approach to utilizing our conventional and molecular methods, we studied the populations of C. difficile in patient stool specimens (n = 23). Our results indicated that 13% of individual stool specimens had heterogeneous populations of C. difficile when we compared the molecular characterization results for multiple bacterial isolates (n = 10). Direct molecular analysis of stool specimens gave results that correlated well with the results obtained with cultured stool specimens; the direct molecular analysis was rapid, informative, and less costly than the testing of multiple patient stool isolates.


Assuntos
Proteínas de Bactérias/genética , Clostridioides difficile/genética , Enterocolite Pseudomembranosa/diagnóstico , Fezes/microbiologia , Reação em Cadeia da Polimerase/métodos , Análise de Sequência de DNA/métodos , Fatores de Virulência/genética , ADP Ribose Transferases/genética , Toxinas Bacterianas/genética , Técnicas de Tipagem Bacteriana , Sequência de Bases , Clostridioides difficile/classificação , Clostridioides difficile/isolamento & purificação , Impressões Digitais de DNA , Eletroforese em Gel de Campo Pulsado , Enterocolite Pseudomembranosa/microbiologia , Enterotoxinas/genética , Variação Genética , Genótipo , Humanos , Dados de Sequência Molecular , New York
15.
Clin Infect Dis ; 47(11): 1372-9, 2008 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-18937575

RESUMO

BACKGROUND: Pharmaceutical compounding, the manipulation of ingredients to create a customized medication, is a widespread practice. In January 2005, the Centers for Disease Control and Prevention was notified of 4 cases of Pseudomonas fluorescens bacteremia that were traced to contaminated heparinized saline intravenous flush syringes prepared as a compounded medical product. PATIENTS AND METHODS: We reviewed medical records of symptomatic patients with P. fluorescens-positive cultures of blood specimens or sections of explanted catheters, reviewed the production process of syringes, performed syringe cultures, compared isolates by pulsed-field gel electrophoresis (PFGE), and examined catheters by scanning electron microscopy. RESULTS: We identified 80 patients in 6 states with P. fluorescens-positive cultures during December 2004-March 2006. Sixty-four patients (80%) had received a diagnosis of cancer. Seventy-four (99%) of 75 patients for whom information about catheter type was available had long-term indwelling catheters. Thirty-three (41%) of 80 cases were diagnosed 84-421 days after the patient's last potential exposure to a contaminated flush (delayed-onset cases). Compared with patients with early infection onset, more patients with delayed infection onset had venous ports (100% versus 50%; P <.001). By PFGE, clinical isolates from 50 (98%) of 51 patients were related to isolates cultured from unopened syringes. Scanning electron microscopy of explanted catheters revealed biofilms containing organisms morphologically consistent with P. fluorescens. CONCLUSION: This outbreak underscores important challenges in ensuring the safety of compounded pharmaceuticals and demonstrates the potential for substantially delayed infections after exposures to contaminated infusates. Exposures to compounded products should be considered when investigating outbreaks. Patients exposed to contaminated infusates require careful follow-up, because infections can occur long after exposure.


Assuntos
Bacteriemia/epidemiologia , Cateteres de Demora/efeitos adversos , Infecção Hospitalar/epidemiologia , Surtos de Doenças , Infecções por Pseudomonas/epidemiologia , Pseudomonas fluorescens/isolamento & purificação , Cloreto de Sódio/efeitos adversos , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Bacteriemia/microbiologia , Técnicas de Tipagem Bacteriana , Sangue/microbiologia , Cateteres de Demora/microbiologia , Criança , Pré-Escolar , Infecção Hospitalar/microbiologia , Eletroforese em Gel de Campo Pulsado , Feminino , Humanos , Lactente , Recém-Nascido , Masculino , Microscopia Eletrônica de Varredura , Pessoa de Meia-Idade , Infecções por Pseudomonas/microbiologia , Pseudomonas fluorescens/classificação , Pseudomonas fluorescens/genética , Cloreto de Sódio/uso terapêutico
16.
Diagn Microbiol Infect Dis ; 62(2): 125-32, 2008 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-18621500

RESUMO

Our laboratory has developed a novel real-time polymerase chain reaction (PCR) assay for the detection of Legionella pneumophila and differentiation from other Legionella spp. in clinical and environmental samples. The 23S rRNA gene was used as a target to detect all Legionella spp., and the mip gene was targeted for the specific detection of L. pneumophila in this multiplex Taqman real-time PCR assay. The 23S rRNA gene is a novel target for Legionella testing; it detects all species and serogroups of Legionella without the contamination issues that accompany the use of the 16S rRNA gene as a target. This assay provides an analytical sensitivity of <1 colony-forming unit and a specificity of 100%. Because culture is important and provides a means for molecular typing via pulsed-field gel electrophoresis (PFGE), we developed a testing algorithm that includes both the new real-time PCR assay and culture for clinical and environmental samples and applied this algorithm during a period of 3 years. Of the 64 clinical samples received by our laboratory for Legionella testing during this period, PCR was found to be an essential diagnostic tool because only 13.3% (2/15) clinical samples that were determined to be L. pneumophila were detected by culture during this period. Of the 276 environmental samples received for Legionella testing during this period, 140 were found to be positive for L. pneumophila. Of these 140 samples, 69.3% were detected by both PCR and culture methods, 29.3% were positive by PCR alone, and 1.4% were positive by culture methods alone. We feel these results indicate that our algorithm, including both PCR and culture, should be used for environmental samples. Among both the clinical and environmental Legionella samples identified by PCR, a subset was not suitable for culture because of issues of lengthy transport, antimicrobial treatment, or bacterial overgrowth. Samples like these are commonly submitted to our laboratory, so the use of our testing algorithm combining these methods is critical. We conclude that molecular and culture methods must be used in combination to provide the best and most comprehensive approach to laboratory detection and investigation of legionellosis.


Assuntos
Microbiologia Ambiental , Legionella pneumophila/isolamento & purificação , Legionella/classificação , Legionella/isolamento & purificação , Legionelose/diagnóstico , Doença dos Legionários/diagnóstico , Reação em Cadeia da Polimerase/métodos , Algoritmos , Meios de Cultura , Humanos , Legionella/genética , Legionella pneumophila/classificação , Legionella pneumophila/genética , Legionelose/microbiologia , Doença dos Legionários/microbiologia , RNA Ribossômico 23S/genética , Sensibilidade e Especificidade , Análise de Sequência de DNA , Fatores de Tempo
17.
Clin Infect Dis ; 45(5): 527-33, 2007 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-17682984

RESUMO

BACKGROUND: In contrast to pharmaceutical manufacturers, compounding pharmacies adhere to different quality-control standards, which may increase the likelihood of undetected outbreaks. In 2005, the Centers for Disease Control and Prevention received reports of cases of Serratia marcescens bloodstream infection occurring in patients who underwent cardiac surgical procedures in Los Angeles, California, and in New Jersey. An investigation was initiated to determine whether there was a common underlying cause. METHODS: A matched case-control study was conducted in Los Angeles. Case record review and environmental testing were conducted in New Jersey. The Centers for Disease Control and Prevention performed a multistate case-finding investigation; isolates were compared using pulsed-field gel electrophoresis analysis. RESULTS: Nationally distributed magnesium sulfate solution (MgSO(4)) from compounding pharmacy X was the only significant risk factor for S. marcescens bloodstream infection (odds ratio, 6.4; 95% confidence interval, 1.1-38.3) among 6 Los Angeles case patients and 18 control subjects. Five New Jersey case patients received MgSO(4) from a single lot produced by compounding pharmacy X; culture of samples from open and unopened 50-mL bags in this lot yielded S. marcescens. Seven additional case patients from 3 different states were identified. Isolates from all 18 case patients and from samples of MgSO(4) demonstrated indistinguishable pulsed-field gel electrophoresis patterns. Compounding pharmacy X voluntarily recalled the product. Neither the pharmacy nor the US Food and Drug Administration could identify a source of contamination in their investigations of compounding pharmacy X. CONCLUSIONS: A multistate outbreak of S. marcescens bloodstream infection was linked to contaminated MgSO(4) distributed nationally by a compounding pharmacy. Health care personnel should take into account the different quality standards and regulation of compounded parenteral medications distributed in large quantities during investigations of outbreaks of bloodstream infection.


Assuntos
Bacteriemia/epidemiologia , Fármacos Cardiovasculares/efeitos adversos , Surtos de Doenças , Contaminação de Medicamentos , Sulfato de Magnésio/efeitos adversos , Infecções por Serratia/etiologia , Serratia marcescens/patogenicidade , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Bacteriemia/microbiologia , Procedimentos Cirúrgicos Cardíacos , Centers for Disease Control and Prevention, U.S./estatística & dados numéricos , Infecção Hospitalar/epidemiologia , Infecção Hospitalar/microbiologia , Composição de Medicamentos/efeitos adversos , Composição de Medicamentos/normas , Feminino , Humanos , Los Angeles/epidemiologia , Masculino , Pessoa de Meia-Idade , New Jersey/epidemiologia , Fatores de Risco , Infecções por Serratia/epidemiologia , Serratia marcescens/isolamento & purificação , Estados Unidos
18.
J Clin Microbiol ; 45(3): 865-73, 2007 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-17202278

RESUMO

A total of 495 temporally and geographically matched Listeria monocytogenes isolates from human clinical cases, foods, ruminant farms, and urban and natural environments were used to investigate L. monocytogenes pulsed-field gel electrophoresis (PFGE) type diversity. Two-enzyme (AscI and ApaI) PFGE discriminated 310 PFGE types and exhibited higher overall discriminatory power (Simpson's index of discrimination [D] = 0.995) than either EcoRI ribotyping (D = 0.950) or AscI or ApaI single-enzyme PFGE (D = 0.992 for both). Seven PFGE types showed significant associations with specific sources, including one and four PFGE types, respectively, associated with human clinical cases and foods. Spatial analysis of 13 PFGE types occurring >5 times showed that two PFGE types were specific to a single processing facility each, where they appear to have persisted over time. Nine PFGE types were geographically widespread and occurred among isolates from multiple sources. For example, a PFGE type that matched isolates from listeriosis outbreaks in Los Angeles and Switzerland occurred among isolates from farms (n = 7), human clinical cases (n = 4), environmental sources (n = 3), and foods (n = 1). Our data indicate that (i) PFGE is highly discriminatory for the subtyping of L. monocytogenes, (ii) some L. monocytogenes PFGE types are associated with specific sources, and (iii) some L. monocytogenes PFGE types are widely distributed and appear to be stable and pandemic. Large PFGE type databases representing isolates from different sources are thus needed to appropriately interpret subtype data in epidemiological investigations and to identify common as well as source-specific PFGE types.


Assuntos
Técnicas de Tipagem Bacteriana , Eletroforese em Gel de Campo Pulsado/métodos , Microbiologia Ambiental , Microbiologia de Alimentos , Listeria monocytogenes/classificação , Listeriose/microbiologia , Criação de Animais Domésticos , Animais , Cidades , Desoxirribonucleases de Sítio Específico do Tipo II/metabolismo , Humanos , Listeria monocytogenes/genética , Listeria monocytogenes/isolamento & purificação , Ruminantes
19.
Clin Infect Dis ; 42(2): 163-9, 2006 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-16355324

RESUMO

BACKGROUND: Foodborne outbreaks of Shigella infection are uncommon and tomatoes are an unusual vehicle. We describe a large, multiple-restaurant outbreak of Shigella flexneri serotype 2a infection that was associated with tomatoes. METHODS: We conducted nationwide surveillance and a case-control study, collected fecal specimens for culture, and measured the survival of the outbreak strain of S. flexneri in tomatoes. RESULTS: We interviewed 306 of 886 ill restaurant patrons and 167 control subjects. Matched univariate analysis showed that several food items were associated with illness, but only tomatoes remained significant in multivariate models. Illness peaked at each restaurant within 24 h after the arrival of hand-sorted bruised and overripe tomatoes from a new distributor; all patient isolates that were tested were indistinguishable by PFGE. Sliced tomatoes from the distributor were inoculated with the outbreak strain, and viable S. flexneri were recovered for 72 h. CONCLUSION: To prevent such outbreaks, persons with shigellosis should be excluded from handling food at all points along the distribution chain.


Assuntos
Surtos de Doenças , Disenteria Bacilar/epidemiologia , Disenteria Bacilar/microbiologia , Microbiologia de Alimentos , Shigella flexneri/isolamento & purificação , Solanum lycopersicum/microbiologia , Adulto , Estudos de Casos e Controles , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , New York/epidemiologia , Vigilância da População , Restaurantes , Shigella flexneri/classificação
20.
Clin Infect Dis ; 42(1): 29-36, 2006 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-16323088

RESUMO

BACKGROUND: Listeriosis, a life-threatening foodborne illness caused by Listeria monocytogenes, affects approximately 2500 Americans annually. Between July and October 2002, an uncommon strain of L. monocytogenes caused an outbreak of listeriosis in 9 states. METHODS: We conducted case finding, a case-control study, and traceback and microbiological investigations to determine the extent and source of the outbreak and to propose control measures. Case patients were infected with the outbreak strain of L. monocytogenes between July and November 2002 in 9 states, and control patients were infected with different L. monocytogenes strains. Outcome measures included food exposure associated with outbreak strain infection and source of the implicated food. RESULTS: Fifty-four case patients were identified; 8 died, and 3 pregnant women had fetal deaths. The case-control study included 38 case patients and 53 control patients. Case patients consumed turkey deli meat much more frequently than did control patients (P = .008, by Wilcoxon rank-sum test). In the 4 weeks before illness, 55% of case patients had eaten deli turkey breast more than 1-2 times, compared with 28% of control patients (odds ratio, 4.5; 95% confidence interval, 1.3-17.1). Investigation of turkey deli meat eaten by case patients led to several turkey processing plants. The outbreak strain was found in the environment of 1 processing plant and in turkey products from a second. Together, the processing plants recalled > 30 million pounds of products. Following the outbreak, the US Department of Agriculture's Food Safety and Inspection Service issued new regulations outlining a L. monocytogenes control and testing program for ready-to-eat meat and poultry processing plants. CONCLUSIONS: Turkey deli meat was the source of a large multistate outbreak of listeriosis. Investigation of this outbreak helped guide policy changes designed to prevent future L. monocytogenes contamination of ready-to-eat meat and poultry products.


Assuntos
Surtos de Doenças , Microbiologia de Alimentos/legislação & jurisprudência , Listeria monocytogenes/isolamento & purificação , Listeriose/epidemiologia , Listeriose/microbiologia , Carne/microbiologia , Adolescente , Adulto , Animais , Estudos de Casos e Controles , Criança , Pré-Escolar , Feminino , Humanos , Lactente , Masculino , Pessoa de Meia-Idade , Perus , Estados Unidos/epidemiologia
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