RESUMO
Håkanson's method of treatment of trigeminal neuralgia by injecting glycerol into the trigeminal cistern is being used in a few clinical centers with encouraging results. We undertook this experimental study utilizing a rat sciatic nerve model to elucidate the mode of action of glycerol. Fifty-six rats were divided into three groups. The topical application of pure glycerol on the sciatic nerve was used in Group I. The intraneural injection of glycerol was used in Group II. The effect of graded concentrations of glycerol was studied in Group III. Physiological saline was the control in Groups I and II. The animals were killed at the end of either 1 or 3 1/2 weeks after the application of glycerol, and the sciatic nerves were examined with light and electron microscopy. Our results show that myelin disintegration and axonolysis occur with glycerol application. Myelinated and unmyelinated fibers are affected at random, although the most striking histological changes were seen in the myelinated fibers.
Assuntos
Glicerol/farmacologia , Nervos Periféricos/efeitos dos fármacos , Animais , Glicerol/administração & dosagem , Glicerol/uso terapêutico , Degeneração Neural/efeitos dos fármacos , Fibras Nervosas/efeitos dos fármacos , Fibras Nervosas/patologia , Fibras Nervosas/ultraestrutura , Fibras Nervosas Mielinizadas/efeitos dos fármacos , Fibras Nervosas Mielinizadas/patologia , Fibras Nervosas Mielinizadas/ultraestrutura , Nervos Periféricos/patologia , Nervos Periféricos/ultraestrutura , Doenças do Sistema Nervoso Periférico/induzido quimicamente , Ratos , Nervo Isquiático/efeitos dos fármacos , Nervo Isquiático/patologia , Nervo Isquiático/ultraestrutura , Neuralgia do Trigêmeo/tratamento farmacológicoRESUMO
An elderly man, without heat in his home for two days, presented with cold, painful, swollen feet. Second-degree frostbite was present. After having moist packs applied to his feet for five days, the patient became febrile and hypotensive, and wet gangrene necessitated left below-knee and right above-knee amputations. Aeromonas hydrophila was isolated from the patient's blood and cultures of the pedal wounds. An etiologic role of the moist pack therapy is postulated.
Assuntos
Infecções Bacterianas/transmissão , Celulite (Flegmão)/transmissão , Infecção Hospitalar/transmissão , Aeromonas , Idoso , Humanos , MasculinoAssuntos
Leucemia Linfoide/etiologia , Animais , Medula Óssea/patologia , Cães , Feminino , Hematócrito , Injeções Subcutâneas , Cariotipagem , Leucemia Linfoide/sangue , Leucemia Linfoide/patologia , Contagem de Leucócitos , Fígado/patologia , Linfonodos/patologia , Linfócitos/citologia , Masculino , Transplante de Neoplasias , Neutrófilos , Veia Porta/patologia , Baço/patologia , Fatores de Tempo , Transplante HomólogoRESUMO
Thirty-four neonatal beagles were inoculated with cells obtained originally from 2 adult beagles, 1 with lymphocytic leukemia, the other with generalized malignant lymphoma. Most of the puppies were irradiated before inoculation; a few were inoculated in utero and were not irradiated. Of the 11 puppies inoculated with buffy coat cells of the blood from the leukemic donor, 2 developed leukemia. Of the 23 pups inoculated with cells of lymph nodes from the spontaneous lymphoma or positive passages, 13 developed lymphoma. Time of measurable onset of leukemia or lymphoma in the recipients varied from 12-60 days after inoculation. One passage was achieved with the leukemia and three serial passages with the lymphoma. A female karyotype was obtained from a neoplastic lymph node in a male recipient of the lymphoma. Since the donor was female, this suggests that the passages of the lymphoma were transplants.
