Machine Learning Approach to Study Social Determinants of Chronic Illness in India: A Comparative Analysis.

BACKGROUND: Several studies on noncommunicable diseases (NCDs) have been carried out worldwide, the basis of most of which is the identification of risk factors-modifiable (or behavioral) and metabolic. Majority of the NCDs are due to sociodemographic factors, lifestyle, and behavior, which can be prevented to a great extent. Thus, it is a health challenge and a necessity to identify such factors of NCDs. OBJECTIVES: The objective is to make a thorough systematic and comparative analysis of diverse machine learning (ML) classifiers and identify the best-performing model to study social determinants of NCDs. MATERIALS AND METHODS: We used data from the Longitudinal Ageing Study in India, and predicted the prevalence of NCDs based on a set of sociodemographic, lifestyle, and behavioral risk factors by conducting a comparative analysis among 25 different algorithms. RESULTS: Evaluating the performance metrics, the random forest model was found to be the most-suited method with 87.9% accuracy and hence chosen as the final model for the analysis. The model's performance was optimized by a hyper-parameter tuning process using grid-search with a 5-fold cross-validation strategy and results suggested that it was able to make accurate predictions on new instances. CONCLUSION: The epidemic of chronic illness cannot be completely addressed without comprehending the social determinants. With advancements in medical and health-care industry, ML has been applied to analyze diseases based on clinical parameters. This work is an attempt by the authors to explore and encourage the use of ML in the field of social epidemiology.

Lesion detection in women breast's dynamic contrast-enhanced magnetic resonance imaging using deep learning.

Breast cancer is one of the most common cancers in women and the second foremost cause of cancer death in women after lung cancer. Recent technological advances in breast cancer treatment offer hope to millions of women in the world. Segmentation of the breast's Dynamic Contrast-Enhanced Magnetic Resonance Imaging (DCE-MRI) is one of the necessary tasks in the diagnosis and detection of breast cancer. Currently, a popular deep learning model, U-Net is extensively used in biomedical image segmentation. This article aims to advance the state of the art and conduct a more in-depth analysis with a focus on the use of various U-Net models in lesion detection in women's breast DCE-MRI. In this article, we perform an empirical study of the effectiveness and efficiency of U-Net and its derived deep learning models including ResUNet, Dense UNet, DUNet, Attention U-Net, UNet++, MultiResUNet, RAUNet, Inception U-Net and U-Net GAN for lesion detection in breast DCE-MRI. All the models are applied to the benchmarked 100 Sagittal T2-Weighted fat-suppressed DCE-MRI slices of 20 patients and their performance is compared. Also, a comparative study has been conducted with V-Net, W-Net, and DeepLabV3+. Non-parametric statistical test Wilcoxon Signed Rank Test is used to analyze the significance of the quantitative results. Furthermore, Multi-Criteria Decision Analysis (MCDA) is used to evaluate overall performance focused on accuracy, precision, sensitivity, F[Formula: see text]-score, specificity, Geometric-Mean, DSC, and false-positive rate. The RAUNet segmentation model achieved a high accuracy of 99.76%, sensitivity of 85.04%, precision of 90.21%, and Dice Similarity Coefficient (DSC) of 85.04% whereas ResNet achieved 99.62% accuracy, 62.26% sensitivity, 99.56% precision, and 72.86% DSC. ResUNet is found to be the most effective model based on MCDA. On the other hand, U-Net GAN takes the least computational time to perform the segmentation task. Both quantitative and qualitative results demonstrate that the ResNet model performs better than other models in segmenting the images and lesion detection, though computational time in achieving the objectives varies.

Stain color translation of multi-domain OSCC histopathology images using attention gated cGAN.

Oral Squamous Cell Carcinoma (OSCC) is the most prevalent type of oral cancer across the globe. Histopathology examination is the gold standard for OSCC examination, where stained histopathology slides help in studying and analyzing the cell structures under a microscope to determine the stages and grading of OSCC. One of the staining methods popularly known as H&E staining is used to produce differential coloration, highlight key tissue features, and improve contrast, which makes cell analysis easier. However, the stained H&E histopathology images exhibit inter and intra-variation due to staining techniques, incubation times, and staining reagents. These variations negatively impact computer-aided diagnosis (CAD) and Machine learning algorithm's accuracy and development. A pre-processing procedure called stain normalization must be employed to reduce stain variance's negative impacts. Numerous state-of-the-art stain normalization methods are introduced. However, a robust multi-domain stain normalization approach is still required because, in a real-world situation, the OSCC histopathology images will include more than two color variations involving several domains. In this paper, a multi-domain stain translation method is proposed. The proposed method is an attention gated generator based on a Conditional Generative Adversarial Network (cGAN) with a novel objective function to enforce color distribution and the perpetual resemblance between the source and target domains. Instead of using WSI scanner images like previous techniques, the proposed method is experimented on OSCC histopathology images obtained by several conventional microscopes coupled with cameras. The proposed method receives the L* channel from the L*a*b* color space in inference mode and generates the G(a*b*) channel, which are color-adapted. The proposed technique uses mappings learned during training phases to translate the source domain to the target domain; mapping are learned using the whole color distribution of the target domain instead of one reference image. The suggested technique outperforms the four state-of-the-art methods in multi-domain OSCC histopathological translation, the claim is supported by results obtained after assessment in both quantitative and qualitative ways.

Potential Therapeutic Agents on Alzheimer's Disease through Molecular Docking and Molecular Dynamics Simulation Study of Plant-Based Compounds.

Globally Alzheimer's disease (AD) is a highly complex, heterogeneous, and multifactorial neurological disease. AD is categorized clinically through a steady loss in memory and progressive decline of cognitive function. So far, there is no effective cure is available for the treatment of AD. Here, we identified Plant-based compounds (PBCs) from seven therapeutic plants through pharmacophore and pharmacokinetics approaches. Subsequently, we retrieved 65 AD associated proteins by Text Mining approach .We observed the interactions between 39 PBCs with 65 AD-associated targets by using molecular docking. Further, we carried out Molecular dynamics simulation analysis to predict the steady binding of top drug-target complexes. The entire MD simulation results analysis was evidence that seven drug-target complexes consistently interacted during the in silico experiment. The top complexes were the target CHLE interacted with 2 PBCs (Pseudojujubogenin and Anahygrine), target VDAC1 interacted with Withanolide R, target THOP1 interacted with Withaolide R, target AOFB interacted with 2 PBCs (Nardostachysin and Viscosalactone B), and target ACHE interacted with the drug (12-Deoxywithastramonolide). These PBCs have stably and flexibly interacted at the protein's active site region. Our results suggest that these PBCs and targets are potential therapeutic candidates for molecular development in AD.

An Ensemble-Based Deep Convolutional Neural Network for Computer-Aided Polyps Identification From Colonoscopy.

Colorectal cancer (CRC) is the third leading cause of cancer death globally. Early detection and removal of precancerous polyps can significantly reduce the chance of CRC patient death. Currently, the polyp detection rate mainly depends on the skill and expertise of gastroenterologists. Over time, unidentified polyps can develop into cancer. Machine learning has recently emerged as a powerful method in assisting clinical diagnosis. Several classification models have been proposed to identify polyps, but their performance has not been comparable to an expert endoscopist yet. Here, we propose a multiple classifier consultation strategy to create an effective and powerful classifier for polyp identification. This strategy benefits from recent findings that different classification models can better learn and extract various information within the image. Therefore, our Ensemble classifier can derive a more consequential decision than each individual classifier. The extracted combined information inherits the ResNet's advantage of residual connection, while it also extracts objects when covered by occlusions through depth-wise separable convolution layer of the Xception model. Here, we applied our strategy to still frames extracted from a colonoscopy video. It outperformed other state-of-the-art techniques with a performance measure greater than 95% in each of the algorithm parameters. Our method will help researchers and gastroenterologists develop clinically applicable, computational-guided tools for colonoscopy screening. It may be extended to other clinical diagnoses that rely on image.

A comparative assessment of deep object detection models for blood smear analysis.

A blood smear is a common type of blood test where blood sample is taken from a patient, smear is made from the sample followed by observation of red blood cells, white blood cells and platelets. A pathologist carefully observes the sample and manually counts the number of RBC, WBC and platelets. This entire process from creating a smear to manually counting each element is tedious and susceptible to human errors. That is why, with the advancement of deep learning, various object detection techniques have become useful for automating the process and mitigating human errors in blood smear analysis. This work presents a comparative assessment of three different object detection models namely Faster R-CNN, EfficientDet D3 and CenterNet Hourglass, and presents their respective inference results. The three models have been compared using the COCO evaluation metrics to identify the best model performance for the given task. It is observed that out of the three models, the Faster R-CNN model performs the best in detecting WBCs and platelets in microscopic blood smear images with an average precision of 99.4%. Critical tasks like medical image processing require accurate predictions to prevent unintended ramifications. Therefore, while slower in terms of inference time, Faster R-CNN is the go-to model where accuracy is the priority. The work is also compared with the existing work in this domain to prove its efficiency.

Breast cancer detection: Shallow convolutional neural network against deep convolutional neural networks based approach.

Introduction: Of all the cancers that afflict women, breast cancer (BC) has the second-highest mortality rate, and it is also believed to be the primary cause of the high death rate. Breast cancer is the most common cancer that affects women globally. There are two types of breast tumors: benign (less harmful and unlikely to become breast cancer) and malignant (which are very dangerous and might result in aberrant cells that could result in cancer). Methods: To find breast abnormalities like masses and micro-calcifications, competent and educated radiologists often examine mammographic images. This study focuses on computer-aided diagnosis to help radiologists make more precise diagnoses of breast cancer. This study aims to compare and examine the performance of the proposed shallow convolutional neural network architecture having different specifications against pre-trained deep convolutional neural network architectures trained on mammography images. Mammogram images are pre-processed in this study's initial attempt to carry out the automatic identification of BC. Thereafter, three different types of shallow convolutional neural networks with representational differences are then fed with the resulting data. In the second method, transfer learning via fine-tuning is used to feed the same collection of images into pre-trained convolutional neural networks VGG19, ResNet50, MobileNet-v2, Inception-v3, Xception, and Inception-ResNet-v2. Results: In our experiment with two datasets, the accuracy for the CBIS-DDSM and INbreast datasets are 80.4%, 89.2%, and 87.8%, 95.1% respectively. Discussion: It can be concluded from the experimental findings that the deep network-based approach with precise tuning outperforms all other state-of-the-art techniques in experiments on both datasets.

Computational learning of features for automated colonic polyp classification.

Shape, texture, and color are critical features for assessing the degree of dysplasia in colonic polyps. A comprehensive analysis of these features is presented in this paper. Shape features are extracted using generic Fourier descriptor. The nonsubsampled contourlet transform is used as texture and color feature descriptor, with different combinations of filters. Analysis of variance (ANOVA) is applied to measure statistical significance of the contribution of different descriptors between two colonic polyps: non-neoplastic and neoplastic. Final descriptors selected after ANOVA are optimized using the fuzzy entropy-based feature ranking algorithm. Finally, classification is performed using Least Square Support Vector Machine and Multi-layer Perceptron with five-fold cross-validation to avoid overfitting. Evaluation of our analytical approach using two datasets suggested that the feature descriptors could efficiently designate a colonic polyp, which subsequently can help the early detection of colorectal carcinoma. Based on the comparison with four deep learning models, we demonstrate that the proposed approach out-performs the existing feature-based methods of colonic polyp identification.

Comparative assessment of CNN architectures for classification of breast FNAC images.

Fine needle aspiration cytology (FNAC) entails using a narrow gauge (25-22 G) needle to collect a sample of a lesion for microscopic examination. It allows a minimally invasive, rapid diagnosis of tissue but does not preserve its histological architecture. FNAC is commonly used for diagnosis of breast cancer, with traditional practice being based on the subjective visual assessment of the breast cytopathology cell samples under a microscope to evaluate the state of various cytological features. Therefore, there are many challenges in maintaining consistency and reproducibility of findings. However, the advent of digital imaging and computational aid in diagnosis can improve the diagnostic accuracy and reduce the effective workload of pathologists. This paper presents a comparison of various deep convolutional neural network (CNN) based fine-tuned transfer learned classification approach for the diagnosis of the cell samples. The proposed approach has been tested using VGG16, VGG19, ResNet-50 and GoogLeNet-V3 (aka Inception V3) architectures of CNN on an image dataset of 212 images (99 benign and 113 malignant), later augmented and cleansed to 2120 images (990 benign and 1130 malignant), where the network was trained using images of 80% cell samples and tested on the rest. This paper presents a comparative assessment of the models giving a new dimension to FNAC study where GoogLeNet-V3 (fine-tuned) achieved an accuracy of 96.25% which is highly satisfactory.

Analysis of Morphological Features of Benign and Malignant Breast Cell Extracted From FNAC Microscopic Image Using the Pearsonian System of Curves.

CONTEXT: Cytological changes in terms of shape and size of nuclei are some of the common morphometric features to study breast cancer, which can be observed by careful screening of fine needle aspiration cytology (FNAC) images. AIMS: This study attempts to categorize a collection of FNAC microscopic images into benign and malignant classes based on family of probability distribution using some morphometric features of cell nuclei. MATERIALS AND METHODS: For this study, features namely area, perimeter, eccentricity, compactness, and circularity of cell nuclei were extracted from FNAC images of both benign and malignant samples using an image processing technique. All experiments were performed on a generated FNAC image database containing 564 malignant (cancerous) and 693 benign (noncancerous) cell level images. The five-set extracted features were reduced to three-set (area, perimeter, and circularity) based on the mean statistic. Finally, the data were fitted to the generalized Pearsonian system of frequency curve, so that the resulting distribution can be used as a statistical model. Pearsonian system is a family of distributions where kappa (κ) is the selection criteria computed as functions of the first four central moments. RESULTS AND CONCLUSIONS: For the benign group, kappa (κ) corresponding to area, perimeter, and circularity was -0.00004, 0.0000, and 0.04155 and for malignant group it was 1016942, 0.01464, and -0.3213, respectively. Thus, the family of distribution related to these features for the benign and malignant group were different, and therefore, characterization of their probability curve will also be different.

Automated classification of Pap smear images to detect cervical dysplasia.

BACKGROUND AND OBJECTIVES: The present study proposes an intelligent system for automatic categorization of Pap smear images to detect cervical dysplasia, which has been an open problem ongoing for last five decades. METHODS: The classification technique is based on shape, texture and color features. It classifies the cervical dysplasia into two-level (normal and abnormal) and three-level (Negative for Intraepithelial Lesion or Malignancy, Low-grade Squamous Intraepithelial Lesion and High-grade Squamous Intraepithelial Lesion) classes reflecting the established Bethesda system of classification used for diagnosis of cancerous or precancerous lesion of cervix. The system is evaluated on two generated databases obtained from two diagnostic centers, one containing 1610 single cervical cells and the other 1320 complete smear level images. The main objective of this database generation is to categorize the images according to the Bethesda system of classification both of which require lots of training and expertise. The system is also trained and tested on the benchmark Herlev University database which is publicly available. In this contribution a new segmentation technique has also been proposed for extracting shape features. Ripplet Type I transform, Histogram first order statistics and Gray Level Co-occurrence Matrix have been used for color and texture features respectively. To improve classification results, ensemble method is used, which integrates the decision of three classifiers. Assessments are performed using 5 fold cross validation. RESULTS: Extended experiments reveal that the proposed system can successfully classify Pap smear images performing significantly better when compared with other existing methods. CONCLUSION: This type of automated cancer classifier will be of particular help in early detection of cancer.