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1.
Int J Biol Macromol ; 100: 89-96, 2017 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-27327907

RESUMO

Curcumin, a diarylheptanoid compound, found in spice turmeric is known to alter the aggregation of proteins and reduce the toxicity of the aggregates. This review looks at the molecular basis of modulating protein aggregation and toxicity of the aggregates. Foremost, we identify the interaction of curcumin and its derivatives with proteins/peptides and the effect of their interaction on the conformational stability and unfolding/folding pathway(s). The unfolding/folding processes generate partially folded/unfolded intermediate, which serve as aggregation precursor state. Secondly, we discuss the effect of curcumin binding on the kinetics parameters of the aggregation process, which give information about the mechanism of the aggregation inhibition. We describe, in addition, that curcumin can accelerate/promote fibril formation by binding to oligomeric intermediate(s) accumulated in the aggregation pathway. Finally, we discuss the correlation of curcumin-induced monomeric and/or oligomeric precursor states with aggregate structure and toxicity. On the basis of these discussions, we propose a model describing curcumin-induced inhibition/promotion of formation of amyloid-like fibrils.


Assuntos
Curcumina/farmacologia , Agregados Proteicos , Proteínas/química , Amiloide/química , Animais , Humanos , Peptídeos/química , Peptídeos/toxicidade , Proteínas/toxicidade
2.
Biochim Biophys Acta ; 1844(3): 670-80, 2014 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-24468532

RESUMO

Interaction of small molecule inhibitors with protein aggregates has been studied extensively, but how these inhibitors modulate aggregation kinetic parameters is little understood. In this work, we investigated the ability of two potential aggregation inhibiting drugs, curcumin and kaempferol, to control the kinetic parameters of aggregation reaction. Using thioflavin T fluorescence and static light scattering, the kinetic parameters such as amplitude, elongation rate constant and lag time of guanidine hydrochloride-induced aggregation reactions of hen egg white lysozyme were studied. We observed a contrasting effect of inhibitors on the kinetic parameters when aggregation reactions were measured by these two probes. The interactions of these inhibitors with hen egg white lysozyme were investigated using fluorescence quench titration method and molecular dynamics simulations coupled with binding free energy calculations. We conclude that both the inhibitors prolong nucleation of amyloid aggregation through binding to region of the protein which is known to form the core of the protein fibril, but once the nucleus is formed the rate of elongation is not affected by the inhibitors. This work would provide insight into the mechanism of aggregation inhibition by these potential drug molecules.


Assuntos
Curcumina/farmacologia , Quempferóis/farmacologia , Muramidase/metabolismo , Dicroísmo Circular , Fluorescência , Guanidina/química , Cinética , Microscopia Eletrônica de Varredura , Simulação de Dinâmica Molecular , Espectrofotometria Ultravioleta
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