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1.
J Assist Reprod Genet ; 37(1): 53-61, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31823133

RESUMO

Even the strictest laboratories and clinics are prone to the occurrence of microbial contamination. In the case of in vitro fertilization (IVF) research and practice facilities, the number of possible sources is particularly vast. In addition to ambient air, personnel, and non-sterilized materials, follicular fluid and semen from patients are a very common gateway for a diverse range of bacteria and fungi into embryo cultures. Even so, reports of contamination cases are rare, what leads many clinics to see the issue as a negligible risk. Microbiological contamination may result in the demise of the patient's embryos, leading to additional costs to both the patient and the clinics. Regardless of financial loss, emotional costs, and stress levels during IVF are highly distressing. Other worrisome consequences include DNA fragmentation, poor-quality embryos, early pregnancy loss or preterm birth, and possible long-term damages that need further investigation. In this review, we aimed to shed a light on the issue that we consider largely underestimated and to be the underlying cause of poor IVF outcomes in many cases. We also discuss the composition of the microbiome and how its interaction with the reproductive tract of IVF-seeking patients might influence their outcomes. In conclusion, we urge clinics to more rigorously identify, register, and report contamination occurrences, and highlight the role of the study of the microbiome to improve overall results and safety of assisted reproduction.


Assuntos
Bactérias/isolamento & purificação , Infecções Bacterianas/economia , Infecções Bacterianas/epidemiologia , Fertilização in vitro/economia , Fertilização in vitro/normas , Técnicas de Reprodução Assistida/economia , Infecções Bacterianas/microbiologia , Feminino , Humanos , Gravidez , Técnicas de Reprodução Assistida/normas
2.
J Assist Reprod Genet ; 36(7): 1339-1349, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31147867

RESUMO

Zika virus (ZIKV) is mainly transmitted through Aedes mosquito bites, but sexual and post-transfusion transmissions have been reported. During acute infection, ZIKV is detectable in most organs and body fluids including human semen. Although it is not currently epidemic, there is a concern that the virus can still reemerge since the male genital tract might harbor persistent reservoirs that could facilitate viral transmission over extended periods, raising concerns among public health and assisted reproductive technologies (ART) experts and professionals. So far, the consensus is that ZIKV infection in the testes or epididymis might affect sperm development and, consequently, male fertility. Still, diagnostic tests have not yet been adapted to resource-restricted countries. This manuscript provides an updated overview of the cellular and molecular mechanisms of ZIKV infection and reviews data on ZIKV persistence in semen and associated risks to the male reproductive system described in human and animal models studies. We provide an updated summary of the impact of the recent ZIKV outbreak on human-ART, weighing on current recommendations and diagnostic approaches, both available and prospective, with special emphasis on mass spectrometry-based biomarker discovery. In the light of the identified gaps in our accumulated knowledge on the subject, we highlight the importance for couples seeking ART to follow the constantly revised guidelines and the need of specific ZIKV diagnosis tools for semen screening to contain ZIKV virus spread and make ART safer.


Assuntos
Biomarcadores , Genitália Masculina/fisiopatologia , Infecção por Zika virus/fisiopatologia , Zika virus/patogenicidade , Animais , Feminino , Genitália Masculina/virologia , Humanos , Masculino , Espectrometria de Massas , Modelos Animais , Sêmen/metabolismo , Infecção por Zika virus/virologia
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