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1.
J Biomed Sci ; 31(1): 81, 2024 Aug 20.
Artigo em Inglês | MEDLINE | ID: mdl-39164686

RESUMO

BACKGROUND: Betulinic acid (BA) has been well investigated for its antiproliferative and mitochondrial pathway-mediated apoptosis-inducing effects on various cancers. However, its poor solubility and off-target activity have limited its utility in clinical trials. Additionally, the immune modulatory role of betulinic acid analogue in the tumor microenvironment (TME) is largely unknown. Here, we designed a potential nanotherapy for colorectal cancer (CRC) with a lead betulinic acid analogue, named as 2c, carrying a 1,2,3-triazole-moiety attached to BA through a linker, found more effective than BA for inhibiting CRC cell lines, and was chosen here for this investigation. Epithelial cell adhesion molecule (EpCAM) is highly overexpressed on the CRC cell membrane. A single-stranded short oligonucleotide sequence, aptamer (Apt), that folds into a 3D-defined architecture can be used as a targeting ligand for its specific binding to a target protein. EpCAM targeting aptamer was designed for site-specific homing of aptamer-conjugated-2c-loaded nanoparticles (Apt-2cNP) at the CRC tumor site to enhance therapeutic potential and reduce off-target toxicity in normal cells. We investigated the in vitro and in vivo therapeutic efficacy and anti-tumorigenic immune response of aptamer conjugated nanotherapy in CRC-TME. METHODS: After the characterization of nanoengineered aptamer conjugated betulinic acid nanotherapy, we evaluated therapeutic efficacy, tumor targeting efficiency, and anti-tumorigenic immune response using cell-based assays and mouse and rat models. RESULTS: We found that Apt-2cNP improved drug bioavailability, enhanced its biological half-life, improved antiproliferative activity, and minimized off-target cytotoxicity. Importantly, in an in vivo TME, Apt-2cNP showed promising signs of anti-tumorigenic immune response (increased mDC/pDC ratio, enhanced M1 macrophage population, and CD8 T-cells). Furthermore, in vivo upregulation of pro-apoptotic while downregulation of anti-apoptotic genes and significant healing efficacy on cancer tissue histopathology suggest that Apt-2cNP had predominantly greater therapeutic potential than the non-aptamer-conjugated nanoparticles and free drug. Moreover, we observed greater tumor accumulation of the radiolabeled Apt-2cNP by live imaging in the CRC rat model. CONCLUSIONS: Enhanced therapeutic efficacy and robust anti-tumorigenic immune response of Apt-2cNP in the CRC-TME are promising indicators of its potential as a prospective therapeutic agent for managing CRC. However, further studies are warranted.


Assuntos
Ácido Betulínico , Neoplasias Colorretais , Molécula de Adesão da Célula Epitelial , Triterpenos Pentacíclicos , Microambiente Tumoral , Neoplasias Colorretais/tratamento farmacológico , Animais , Microambiente Tumoral/efeitos dos fármacos , Camundongos , Triterpenos Pentacíclicos/farmacologia , Molécula de Adesão da Célula Epitelial/metabolismo , Humanos , Nanopartículas/química , Linhagem Celular Tumoral , Ratos
2.
Plant Physiol Biochem ; 207: 108388, 2024 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-38295528

RESUMO

Grass pea has the potential to become a miracle crop if the stigma attached to it as a toxic plant is ignored. In light of the following, we conducted transcriptome analyses on the high and low ODAP-containing cultivars i.e., Nirmal and Bidhan respectively in both normal and salt stress conditions. In this study, genes that work upstream and downstream to ß-ODAP have been found. Among these genes, AAO3 and ACL5 were related to ABA and polyamine biosynthesis, showing the relevance of ABA and polyamines in boosting the ß-ODAP content in Nirmal. Elevated ß-ODAP levels in salt stress-treated Bidhan may have evolved tolerance by positively regulating the expression of genes involved in phenylpropanoid and jasmonic acid biosynthesis. Although the concentration of ß-ODAP in Bidhan increased under salt stress, it was lower than in stress-treated Nirmal. Despite this, the expression of stress-related genes that work downstream to ß-ODAP was found higher in stress-treated Bidhan. This could be because stress-treated Nirmal has lower GSH, proline, and higher H2O2, resulting in the development of severe oxidative stress. Overall, our research not only identified new genes linked with ß-ODAP, but also revealed the molecular mechanism by which a low ß-ODAP-containing cultivar developed tolerance against salinity stress.


Assuntos
Diamino Aminoácidos , Lathyrus , Lathyrus/genética , Lathyrus/metabolismo , Neurotoxinas/análise , Neurotoxinas/metabolismo , Diamino Aminoácidos/análise , Diamino Aminoácidos/metabolismo , Peróxido de Hidrogênio/metabolismo , Estresse Salino/genética
3.
J Biosci ; 472022.
Artigo em Inglês | MEDLINE | ID: mdl-36503908

RESUMO

Plants are frequently exposed to a plethora of unfavourable climatic catastrophes, be it abiotic or biotic stresses, viz., salinity, water (drought or water logging), extreme temperature, heavy metal, nutrient deficiency, ozone, pathogen attack, etc., which badly affect the yield and productivity of crops. Plants, as part of their defence machinery, employ different tolerance mechanisms to survive under adverse conditions. In addition to other stress responses, the mitogen-activated protein kinase (MAPK) signalling cascade and accumulation of glutathione (GSH) are two important aspects of plant defence response. Induction of the MAPK cascade is one of the earliest responses when a plant is under any environmental stress, and there is documentary evidence of this signalling pathway, in turn, regulating various phytohormone-signalling networks and other defence-related pathways during stress. Similarly, GSH being a low molecular weight metabolite also has a key role in environmental stress tolerance. It is known to be involved in multi-step interactions with various phytohormones, many signalling molecules, and redox molecules such as reactive oxygen species (ROS). This review provides an outline on GSH-MAPK crosstalk to better understand its role in the context of defence signalling in plants.


Assuntos
Glutationa , Transdução de Sinais , Reguladores de Crescimento de Plantas , Secas , Sistema de Sinalização das MAP Quinases
4.
Plant Signal Behav ; 17(1): 2047352, 2022 12 31.
Artigo em Inglês | MEDLINE | ID: mdl-36184871

RESUMO

Glutathione (GSH) is well known to play a crucial role in imparting resistance against various pathogen invasions. Nevertheless, the role of GSH in regulating miRNA-mediated defense response is yet to be explored. To decipher the GSH-mediated regulation of miRNA expression during necrotrophic infection in Arabidopsis thaliana, wild-type Col-0 and AtECS1, the transgenic line exhibiting enhanced GSH content, were infected with necrotrophic pathogen Alternaria brassicicola. AtECS1 plants exhibited enhanced resistance as compared to wild-type. MiRNA next-generation sequencing (NGS) was performed to compare the miRNA expression in Col-0 and AtECS1 leaves. Under control condition, differentially expressed 96 known miRNAs and 17 novel miRNAs viz. ath-miR8167f, ath-miR1886.3, ath-miR3932b-5p, etc. were identified. However, under infected condition, 73 known and 43 novel differentially expressed miRNAs viz. ath-miR5652, ath-miR160b, ath-miR865-5p, etc. were identified. Functional annotation and enrichment analysis revealed that several miRNAs that target defense-related genes like leucine-rich repeat protein kinase, MYB transcription factors, TCP8, etc. were down regulated in the AtECS1 line, which, in turn, relieves the repression of their target gene expression, leading to resistance against infection. Together, the present investigation suggests that GSH plays a decisive role in modulating the miRNA-mediated regulation of defense-related genes during pathogen invasion.


Assuntos
Proteínas de Arabidopsis , Arabidopsis , MicroRNAs , Alternaria , Arabidopsis/genética , Arabidopsis/metabolismo , Proteínas de Arabidopsis/genética , Proteínas de Arabidopsis/metabolismo , Glutationa/metabolismo , MicroRNAs/genética , MicroRNAs/metabolismo , Doenças das Plantas/genética , Fatores de Transcrição/metabolismo
5.
J Plant Physiol ; 271: 153664, 2022 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-35279560

RESUMO

Glutathione (GSH) plays a fundamental role in plant defense. Recent reports showed that enhanced GSH content activates mitogen-activated protein kinases (MPKs). However, the molecular mechanism behind this GSH-mediated MPKs expression during environmental challenges is unexplored. Here, we found that under control and combined abiotic stress-treated conditions, GSH feeding activates MPK3 expression in Arabidopsis thaliana by inducing its promoter, as established through the promoter activation assay. Additionally, transgenic A. thaliana overexpressing the LeMPK3 gene (AtMPK3 line) showed increased γ-ECS expression, which was decreased in mpk3, the MPK3-depleted mutant. An in-gel kinase assay exhibited hyperphosphorylation of Myelin Basic Protein (MBP) in the GSH-fed AtMPK3 transgenic line. Under control and combined abiotic stress treated conditions, expression of transcription factor WRKY40 binding to MPK3 promoter was up-regulated under enhanced GSH condition. Interestingly, GSH feeding was rendered ineffective in altering MPK3 expression in the Atwrky40 mutant, emphasizing the involvement of WRKY40 in GSH-MPK3 interplay. This was further confirmed by a wrky40 co-transformation assay. The immunoprecipitation assay followed by ChIP-qPCR showed a significant increase in the binding of WRKY40 to MPK3 promoter, which further established MPK3-WRKY40 association upon GSH feeding. In conclusion, this study demonstrated that GSH modulates MPK3 expression via WRKY40 in response to stress.


Assuntos
Proteínas de Arabidopsis , Arabidopsis , Arabidopsis/genética , Arabidopsis/metabolismo , Proteínas de Arabidopsis/genética , Proteínas de Arabidopsis/metabolismo , Resposta ao Choque Frio , Regulação da Expressão Gênica de Plantas , Glutationa/metabolismo , Proteína Quinase 3 Ativada por Mitógeno/metabolismo , Quinases de Proteína Quinase Ativadas por Mitógeno/genética , Quinases de Proteína Quinase Ativadas por Mitógeno/metabolismo , Plantas Geneticamente Modificadas/genética , Fatores de Transcrição/metabolismo
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