RESUMO
The prospective study explored the hemodynamic effects of colloidal solution replacement therapy and the criteria for its safety in patients with acute lung parenchymatous lesions (ALPL) attended by hypoalbuminemia and coagulopathy. There were 68 observations of the effects of colloidal solutions: 20% albumin solution (n=25), freshly frozen plasma (FFP) (n=20), 6% hydroxyethylated starch (HES) 130/0.4 9:1 (n=23). The colloidal solutions were infused at a constant rate; the infusion was stopped until pulmonary wedge pressure (PWP) was 25% greater than its baseline value. Before and after infusion, the parameters of central hemodynamics and oxygen transport, extravascular lung water index (ELWI), pulmonary vascular permeability index (PVPI), and colloid-osmotic pressure (COP) were measured. The infusion volumes were 3.8 +/- 0.4, 13.7 +/- 1.4, and 13.4 +/- 1.3 ml/kg for 20% albumin solution, 6% HES 130/0.4, and FFP, respectively. The PWP-COP gradient increased in all groups. After FFP infusion, there was an increase in ELWI and lung shunt. After 20% albumin solution, there was a delayed increase in ELWI. There was no rise in ELWI after 6% HES administration. In the 20% albumin solution group, the increased ELWI was recorded in patients who had positive baseline PWP-COP gradients (p < 0.05). A combination of higher PVPI and a positive PWP-COP value causes a greater increase in ELWI after 20% albumin solution infusion than in the normal PVPI group. In patients with ALPL, FFP infusion may lead to an increase in the accumulation of extravascular lung water. A negative preinfusion PWP-COP gradient is a safety criterion for the infusion of 20% albumin solution in patients with ALPL. The increased PVPI in combination with a positive PWP-COP gradient is an aggravating factor.
Assuntos
Coloides/efeitos adversos , Hemodiluição , Pneumopatias/terapia , Substitutos do Plasma/efeitos adversos , Doença Aguda , Permeabilidade Capilar , Coloides/administração & dosagem , Hemodinâmica/efeitos dos fármacos , Humanos , Pneumopatias/etiologia , Pneumopatias/fisiopatologia , Oxigênio/sangue , Substitutos do Plasma/administração & dosagem , Estudos Prospectivos , Troca Gasosa Pulmonar , Segurança , Albumina Sérica/metabolismo , Equilíbrio HidroeletrolíticoRESUMO
Using P 388 and P 388/Dx tumour-bearing mice BDF1 it has been studied effect Tritton X-100 on accumulation and therapeutic action of doxorubicin (Dx). It has been shown that LD50 of Tritton X-100 is 153.6 mg/kg and MTD is 80 mg/kg body weight of animals. It has been shown that Tritton X-100 in dose 40 mg/kg body weight increases initial level of Dx in P 388/Dx cells to 215% and doesn't change accumulation of Dx in P 388 cells. It has been shown that Tritton X-100 doesn't influence the therapeutic effect of Dx in P 388 and P 388/Dx tumour-bearing mice.
Assuntos
Doxorrubicina/farmacologia , Leucemia P388/tratamento farmacológico , Polietilenoglicóis/farmacologia , Animais , Sobrevivência Celular/efeitos dos fármacos , Doxorrubicina/uso terapêutico , Resistência a Medicamentos , Sinergismo Farmacológico , Camundongos , OctoxinolRESUMO
We have studied by uridine short term test the level of resistance of murine leukemia cell lines P 388/Dx and ELD/Dx carcinoma cells with induced resistance to doxorubicin, P 388/Fp + Dx cells with induced resistance to combination of finoptOFF++ and doxorubicin in vivo. It was shown that the level of resistance was 6 fold for P 388/Dx cells, 4.5 fold for ELD/Dx cells and 2 fold for P 388/Fp + Dx cells. It was shown that the P 388/Dx cells and P 388/Fr + Dx cells had a 3.5 and 4.4 fold increase level of glutathione-S-transferase activity than P 388 cells. No increase in the activity of glutathione-S-transferase was detected in ELD/Dx cells. We conclude that increase of cellular glutathione-S-transferase activity is not associated with the development of resistance to doxorubicin.
Assuntos
Carcinoma de Ehrlich/tratamento farmacológico , Doxorrubicina/uso terapêutico , Glutationa Transferase/análise , Leucemia P388/tratamento farmacológico , Animais , Carcinoma de Ehrlich/enzimologia , Resistência a Medicamentos , Leucemia P388/enzimologia , Células Tumorais Cultivadas , Verapamil/uso terapêuticoRESUMO
Phynoptin (Ph) and cyclophosphamide (CP) gave rise to a type I spectral changes with liver microsomal fraction. KS were 15 microM and 2150 microM, respectively. Ph increases the concentration of NBP product(s) of CP and acrolein in the blood plasma of animals. Ph increases a toxicity of CP. LD50 was 388.0 +/- 13.9 mg/kg for CP and LD50 was 342.8 +/- 16.9 mg/kg for CP in combination with Ph. Ph changes a therapeutic action of CP in mice with hemocytoblastosis La. Pharmacokinetic interactions have been demonstrated between calcium antagonists Ph and CP.
Assuntos
Ciclofosfamida/farmacocinética , Verapamil/farmacologia , Animais , Ciclofosfamida/farmacologia , Ciclofosfamida/uso terapêutico , Relação Dose-Resposta a Droga , Interações Medicamentosas , Ensaios de Seleção de Medicamentos Antitumorais , Técnicas In Vitro , Leucemia Experimental/sangue , Leucemia Experimental/tratamento farmacológico , Leucemia Experimental/mortalidade , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Endogâmicos CBA , Microssomos Hepáticos/efeitos dos fármacos , Microssomos Hepáticos/metabolismo , Transplante de Neoplasias , Verapamil/uso terapêuticoRESUMO
beta-D-galactose-containing glycoproteins were prepared from cells P-388 leukemia and from P-388 leukemia cells with induced resistance to doxorubicin. It was shown by HPLC method that plasma membranes from resistant cells contain 4-4.5% P-glycoproteins and plasma membranes from sensitive cells contain P-glycoproteins about 10 times lower.
Assuntos
Doxorrubicina/antagonistas & inibidores , Regulação Neoplásica da Expressão Gênica/fisiologia , Leucemia P388/genética , Glicoproteínas de Membrana/genética , Proteínas de Neoplasias/genética , Membro 1 da Subfamília B de Cassetes de Ligação de ATP , Animais , Cromatografia de Afinidade , Cromatografia Líquida de Alta Pressão , Resistência a Medicamentos , Immunoblotting , Leucemia P388/metabolismo , Glicoproteínas de Membrana/análise , Glicoproteínas de Membrana/isolamento & purificação , Proteínas de Neoplasias/análise , Proteínas de Neoplasias/isolamento & purificação , SolubilidadeRESUMO
The authors studied accumulation of the fluorescent probe Hoechst 33258 in leukemia P 388 sensitive (P 388/0) and resistant to doxorubicin (P 388/DOX) cells. It was shown that intensity of fluorescence of the dye increased after binding with nuclear DNA during 25 min for both lines of the cells. Intensity of fluorescence was 40% greater in sensitive than resistant cells. If Triton X-100 was added no difference between two lines of the cell was observed. When doxorubicin was added to the cells with dye, the intensity of fluorescence decreased. It was suggested to use Hoechst 33258 for assessment extent doxorubicin accumulation in nuclei of the cells.
Assuntos
Doxorrubicina/uso terapêutico , Corantes Fluorescentes , Leucemia P388/tratamento farmacológico , Animais , Núcleo Celular/metabolismo , Doxorrubicina/farmacocinética , Resistência a Medicamentos , Ensaios de Seleção de Medicamentos Antitumorais , Leucemia P388/patologia , Masculino , Camundongos , Octoxinol , Polietilenoglicóis , Espectrometria de FluorescênciaRESUMO
Approximately a 1.6-fold increase in the antitumor action of doxorubicin used in combination with artificial hyperglycemia was shown on mice C57B1/6 with hemocytoblastosis La. Artificial hyperglycemia was found to change the doxorubicin pharmacokinetics in the experimental animals evident from increased in antibiotic half-life to 42.3 min against 26.5 min in the controls, the apparent initial concentration of doxorubicin being increased 1.6 times. Accumulation of doxorubicin in the bone marrow cells of the mice did not change with artificial hyperglycemia. It was suggested that the increase in the therapeutic effect of doxorubicin used in combination with artificial hyperglycemia was associated with changes in drug pharmacokinetics.
Assuntos
Doxorrubicina/administração & dosagem , Glucose/administração & dosagem , Hiperglicemia/metabolismo , Leucemia Experimental/tratamento farmacológico , Leucemia-Linfoma Linfoblástico de Células Precursoras/tratamento farmacológico , Animais , Doxorrubicina/farmacocinética , Ensaios de Seleção de Medicamentos Antitumorais , Sinergismo Farmacológico , Quimioterapia Combinada , Leucemia Experimental/metabolismo , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Endogâmicos CBA , Leucemia-Linfoma Linfoblástico de Células Precursoras/metabolismo , Fatores de TempoRESUMO
Using hybrid mice BDF1 doxorubicin (Dx) accumulation has been determined in leukemia P388 cells (P388/0), P388 cells with induced resistance to Dx (P388/Dx) and P388 cells with induced resistance to the finoptin (Fp) + Dx combination (P388/Fp + Dx). It has been shown that Fp doesn't affect Dx accumulation in or elimination from leukemia cells P388/0 or P388/Fp + Dx. The resistance of P388/Fp + Dx cells to the Fp + Dx combination develops during 6 passages. It can be concluded that Fp application doesn't abolish the problem of tumor cells' resistance to cytostatics.
Assuntos
Protocolos de Quimioterapia Combinada Antineoplásica/farmacologia , Leucemia P388/tratamento farmacológico , Leucemia Experimental/tratamento farmacológico , Animais , Protocolos de Quimioterapia Combinada Antineoplásica/antagonistas & inibidores , Protocolos de Quimioterapia Combinada Antineoplásica/farmacocinética , Protocolos de Quimioterapia Combinada Antineoplásica/uso terapêutico , Doxorrubicina/administração & dosagem , Doxorrubicina/antagonistas & inibidores , Doxorrubicina/farmacocinética , Interações Medicamentosas , Resistência a Medicamentos , Ensaios de Seleção de Medicamentos Antitumorais , Leucemia P388/metabolismo , Leucemia P388/mortalidade , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Endogâmicos DBA , Fatores de Tempo , Verapamil/administração & dosagem , Verapamil/antagonistas & inibidores , Verapamil/farmacologiaRESUMO
Using mice BDF1 it has been shown that the period of retention of Doxorubicin (Dx) is shorter in the leukemia P388 cells with induced antibiotic resistance (P388/Dx) as compared to P388 cells sensitive to Dx. Administration of Verapamil (Vp) to animals leads to an increase of Dx concentration in the leukemia P388/Dx cells during a 240 min observation period. Vp promotes the therapeutic effect of Dx on P388/Dx bearing mice. It can be suggested that the mechanism of Vp action consists in the damaged Dx elimination from cells with induced resistance, since Vp doesn't change the period of circulation of the antibiotic in the blood plasma of mice.
Assuntos
Doxorrubicina/farmacocinética , Leucemia P388/metabolismo , Leucemia Experimental/metabolismo , Verapamil/farmacologia , Animais , Resistência Microbiana a Medicamentos , Masculino , CamundongosRESUMO
Using male mice BDF1, it has been shown that the retention period of doxorubicin (DOX) is shorter in the leukemia P 388 cells with induced antibiotic resistance (P 388/DOX) as compared to the P 388 cells, sensitive to DOX. Administration of finoptin (FP) to animals leads to the increase of DOX concentration in the leukemia P 388/DOX cells during 240 min observation. FP promotes the therapeutic effect of DOX on mice bearing leukemia P 388/DOX. It can be suggested that the mechanism of FP action is the damaged DOX elimination from cells with induced resistance, since FP doesn't change the period of antibiotic circulation in the murine blood plasma.
Assuntos
Doxorrubicina/farmacocinética , Leucemia P388/tratamento farmacológico , Leucemia Experimental/tratamento farmacológico , Verapamil/uso terapêutico , Animais , Doxorrubicina/análise , Doxorrubicina/antagonistas & inibidores , Interações Medicamentosas , Resistência Microbiana a Medicamentos , Ensaios de Seleção de Medicamentos Antitumorais , Quimioterapia Combinada , Leucemia P388/metabolismo , Masculino , Camundongos , Camundongos Endogâmicos , Transplante de Neoplasias , Fatores de TempoRESUMO
We have studied amino acid up-take from incubation media by colchicine-resistant and colchicine-sensitive Djungarian hamster fibroblast cells. It has been shown that arginine and asparagine amino acid contents in the medium are different for colchicine-sensitive and colchicine-resistant cells. Amino acids concentration is not reduced in the medium after incubation of resistant cells, while it is decreased after incubation of sensitive cells. We can suggest that penetration of low-weight sources of nitrogen into sensitive cells is hampered. It can also be suggested that protein macromolecules are the main source of nitrogen for these cells. The protein up-take levels from the incubation medium, as assessed by the ammonia and amino acid contents of cell counts don't exceed 5% of their initial concentrations in the medium.