[Proliferative processes in the epithelium of colon mucosa in patients with Salmonella infection during first 24 hours after the onset of the disease]. / Proliferativnye protsessy v épitelii slizistoi obolochki tolstoi kishki u bol'nykh sal'monellezom v pervye sutki bolezni.

DNA synthesis in the epithelium of a distal portion of human colon was studied by radioautography with 3H-thymidine in patients during the first 24 hours of gastrointestinal salmonellosis. All 13 patients aged 18-50 years were admitted to hospital during 9-20 hours after the disease onset. Nobody had a background of gastrointestinal pathology. The control group consisted of samples from sigmoid colon epithelium of 30 healthy individuals. The labelled nuclei index (LNI) was 5.16 +/- 0.27%, the intensity of labelling (IL) was 13.65x +/- 0.82. Endoscopic and pathomorphological studies of colonic mucosa in salmonellosis by the end of the first 24 hours of the disease showed superficial inflammation. Increased LNI (11.48 +/- 1.07%, p < 0.05) characterized activation of proliferation and increased IL (22.24 +/- 2.1, p < 0.05) showed intensification of DNA synthesis. Additionally, in one third of cases enlargement of proliferation compartment up to 2/3 crypt was noticed. It is unlikely that in this situation immune mechanisms play the key role, it seems that a direct stimulation of proliferative processes by a bacterial agent takes place.

[Characteristic of proliferative processes in colonic mucosa in acute salmonellosis]. / Kharakteristika protsessov proliferatsii épiteliia slizistoi obolochki tolstoi kishki v ostrom periode sal'monelleza.

DNA synthesis in the epithelium of a distal portion of human colon was studied at radioautography with 3H-thymidine through the first three days of gastrointestinal salmonellesis. Control studies were performed on the mucosa of the sigmoid colon of healthy persons undergoing rectoromanoscopy because of epidemiological reasons. No pathological changes were found in these specimens, the labeled nuclei index (LNI) was 5.16 +/- 0.27%, the label intensity (LI) which characterizes the rate of DNA synthesis was 13.65 +/- 0.82. Group 1 consisted of 18 patients having slight inflammation without erosions, ulcerative and haemorrhagic changes. We found significant activation of proliferation (LNI = 15.09 +/- 0.9%, LI = 22.23 +/- 1.9; p < 0.05) in this group compared to the control one. 15 patients with salmonella-induced hemorrhagic colitis formed group 2 in which LNI and LI were also increased (LNI = 9.89 +/- 1.0%, LI = 19.3 +/- 2.3; p < 0.02) vs. control group, but these changes seemed to be significantly weaker than those in group 1 (p < 0.01). It is inferred that rates of proliferation in colon epithelium in acute salmonellosis are significantly higher than in healthy epithelium but activity of these processes decreases with growing severity of inflammation.

[The epithelial proliferative activity of the mucosa of the large intestine in dysentery patients at the acute period of the disease depending on the expression of morphological changes]. / Proliferativnaia aktivnost' épiteliia slizistoi obolochki tolstoi kishki bol'nykh dizenteriei v ostrom periode bolezni v zavisimosti ot vyrazhennosti morfologicheskikh izmenenii.

The dependence of proliferative activity on the degree of histomorphological changes in colonic mucosa of patients with dysentery was studied histoautoradiographically in colonic biopsies. Morphological changes were evaluated according to N. B. Shalygina's schema (catarrhal, exudative, erosive or ulcerous inflammation) A 2.5-3-fold increase of the labelled nuclei index (LNI) and label intensity (LI) was found in the catarrhal inflammation. LNI was increased 1.2-fold and LI 1.5-fold in cases of other types of inflammation; they were lower than in catarrhal inflammation.

[Disturbance of epithelial proliferation of the gastro-intestinal tract mucosa in ulcers and erosion]. / Narusheniia protsessov proliferatsii épiteliia slizistoi obolochki zheludochno-kishechnogo trakta pri iazvakh i éroziiakh.

Epithelial proliferation in the mucosa of the gastro-intestinal tract was studied autoradiographically with 3H-thymidine under the following conditions: acute gastric ulcers as complication of acute pancreatitis; exacerbation of duodenal ulcer; ulcers and erosions in bacterial dysentery. Mucosa of practically healthy persons as well as mucosa of patients with the same diseases not complicated with ulcers and erosions served as control. Biopsies of mucosa were taken 2 cm from ulcers and erosions. The decrease of DNA-synthesizing nuclei number in the mucosa epithelium at early stages of ulcer formation was observed in all these conditions.

[The characteristics of the proliferative processes in the epithelium of the sigmoid intestine in acute bacillary dysentery]. / Kharakteristika proliferativnykh protsessov v épitelii sigmoidnoi kishki pri ostroi bakterial'noi dizenterii.

Proliferative processes were studied using radioautography with 3H-thymidine at the height of the disease and in the early convalescence of the bacterial dysentery. The mucous membrane of the sigmoid colon of healthy persons undergoing rectoromanoscopy due to the epidemic reasons served as control. No pathologic changes were found in these healthy persons; the labeled nuclei index (LNI) was 5.75 +/- 0.4%, the label intensity (LI) characterizing the rate of DNA synthesis was 14.9 +/- 0.8. During the peak of bacterial dysentery LNI increased up to 12.9 +/- 1.3% and there was a three-fold increase of LI. Activation of the proliferative processes persisted in the period of an early recovery. LNI was 9.4 +/- 1.0%, LI was 2.6 times higher as compared to control.