The Effect of Matrix Metalloproteinase Inhibitors on the Microtensile Bond Strength of Dentin Bonding Agents in Caries Affected Dentin: A Systematic Review.

Aims and Objectives: Matrix metalloproteinases (MMPs) cause degradation of the dentinal matrix, as they act actively on collagen fibrils, leading to their deterioration and collapse. MMP inhibitors are known to be used for the pre-treatment of human dentin before bonding. Most studies on the MMP inhibitors examined the effect of MMP inhibitors on bonding to sound dentin (SD), but few examine their effect on bonding to caries affected dentin (CAD). This systematic review aims to identify and summarize studies that have applied MMP inhibitors for pre-treatment of CAD, and examine the microtensile bond strength (µTBS), bond durability, and the mode of failure. Materials and Methods: A systematic review was performed using the PubMed database according to the PRISMA guidelines. A total of 785 original articles published between 2010 and 2022 were initially retrieved. Six studies were selected based on predefined inclusion-exclusion criteria, and their outcomes were extracted and analyzed. The methodological quality assessment was performed using a combined checklist that utilizes the reporting criteria mentioned in the checklist for reporting in-vitro studies guidelines and guidelines for reporting pre-clinical in vitro studies on dental materials. Results: All six studies included here showed a definitive increase of the µTBS when MMP inhibitors were applied to the CAD. The mode of failure was found to be predominantly adhesive in nature. The deviation in the values of µTBS was approximately 2-5 MPa on immediate and delayed testing. Conclusion: MMP-inhibiting agents could be considered for the pretreatment of teeth with CAD as a part of their tooth preparation area, thereby allowing the clinician to retain CAD and bond to the CAD without endangering the vital pulp.

Comparative evaluation of the effect of 10% sodium ascorbate, 10% hesperidin, 1% riboflavin 5 phosphate, collagen cross-linkers, on the pushout bond strength of fiber postluted to radicular dentin: In vitro study.

AIMS: The aim of the study was to evaluate the effect of 10% sodium ascorbate (SA),10% hesperidin, 1% riboflavin 5-phosphate, collagen cross-linkers, on the pushout bond strength of the fiber resin postluted to the radicular dentin. MATERIALS AND METHODS: Twenty-eight, single-rooted extracted human teeth were collected. The cleaning shaping and obturation was done followed postspace preparation which was etched with 37% phosphoric acid for 15 s and then rinsed with normal saline. The specimens were divided into four groups and postspaces were irrigated, respectively with. (i) Group 1: Normal saline for 4 min, (ii) Group 2: 10% SA for 4 min, (iii) Group 3: 10% hesperidin for 4 min, and (iv) Group 4: 1% riboflavin 5 phosphate (RFV) for 4 min. The pushout bond strength of fiber postluted to root dentin, of all the above groups, was compared. Posts were luted using Rely X Arc luting cement. Each root was horizontally sectioned to obtain 2 mm thick, 28 dentinal disks, per group. The pushout bond strength test was carried out using Universal Testing Machine. The scores were statistically analyzed using Student's Unpaired "t" and ANOVA test. RESULTS: Comparison of different collagen crosslinker showed that the highest pushout bond strength value was recorded with 10% hesperidin, followed by 1% RFV and 10% SA. CONCLUSION: All collagen cross-linkers so used, increased the bond strength. The highest push-out bond strength was achieved with 10% hesperidin.