RESUMO
The incubation of quantum dots with lymphocytes induced significant changes in all morphometric characteristics of these cells. Protein crown formed on the surface of quantum dots after incubation with the serum consists mainly of transport proteins, immunoglobulins, blood coagulation proteins, and kininogens. Protein crown changes the morphometric characteristics of cells: in the case of incubation with quantum dots that have low-molecular-weight coating, a shift towards control parameters (cells without exposure) was observed; on the contrary, after incubation with quantum dots that have a high-molecular-weight coating, the differences from the control became more pronounced. It can be hypothesized that protein crown provokes autoagression of lymphocytes against each other and against platelets.
Assuntos
Linfócitos/ultraestrutura , Pontos Quânticos , Adulto , Humanos , Microscopia de Força Atômica , Microscopia Eletrônica de Varredura , Adulto JovemRESUMO
Studies by flow cytometry, scanning electron microscopy, and X-ray microanalysis have shown that fixation/internalization of quantum dots differ in lymphocytes and neutrophilic granulocytes. Punctate fixation of quantum dots to cell surface is characteristic of lymphocytes, while phagocytosis with perinuclear location of quantum dot aggregations is characteristic of neutrophils. The presence of complex functional groups on the surface of quantum dots can inhibit significantly the fixation/internalization of quantum dots by blood cells.
Assuntos
Granulócitos/metabolismo , Linfócitos/metabolismo , Neutrófilos/metabolismo , Pontos Quânticos/metabolismo , Adulto , Células Cultivadas , Humanos , FagocitoseRESUMO
This article describes the main morphological and biochemical features of neutrophil granulocytes in the process of phagocytosis of two types of quantum dots (CdSe/ZnS-MPA and CdSe/CdSeZnS/ZnS-PTVP). This study is the first to observe the transfer of large aggregates of quantum dots through neutrophils. During this process the cells demonstrate high chemotactic activity. Furthermore, neutrophil pseudopodia underwent alterations during the early stages of phagocytosis. The findings demonstrated that the biochemical profile of neutrophils is practically identical after phagocytosis of both types of quantum dots, but differs significantly from neutrophil metabolism after bacterial phagocytosis.
RESUMO
A bulk nanostructured material based on oxidized silicon nanopowder was fabricated using a spark plasma sintering technique. Structural investigations revealed that this material has the composition of â¼14 nm core Si granules inside an SiO2 shell. Photoluminescence measurements have shown that the emission spectra lie in the energy range of 0.6-1.1 eV, which is not typical of the emissions of the Si/SiO2 nanostructures reported in numerous papers. This result can be explained by the formation of energy states in the bandgap and the participation of these states in both electronic transport and photoluminescence emission. Annealing of the sample leads to a decrease in defect density, which in turn leads to quenching of the 0.6-1.1 eV photoluminescence. In this case â¼1.13 eV inter-band transitions in the Si core start to play a dominant role in radiative recombination. Thus, the possibility of controlling the photoluminescence emission over a broad wavelength range was demonstrated.