RESUMO
Tissue samples from the feet of slaughtered cattle exhibiting different stages of digital dermatitis were sectioned and stained with haematoxylin and eosin and silver staining techniques. Three morphological variations of spirochaetes were observed, whereas control samples from feet which were macroscopically negative for digital dermatitis were also negative for spirochaetes. In an immunofluorescence test, Campylobacter faecalis was found to be abundant on superficial wound smears from the classical ulceration of digital dermatitis.
Assuntos
Infecções por Campylobacter/veterinária , Doenças dos Bovinos/microbiologia , Doenças dos Bovinos/patologia , Dermatoses do Pé/veterinária , Casco e Garras/patologia , Infecções por Spirochaetales/microbiologia , Infecções por Spirochaetales/veterinária , Animais , Campylobacter/isolamento & purificação , Infecções por Campylobacter/microbiologia , Infecções por Campylobacter/patologia , Bovinos , Corantes , Feminino , Imunofluorescência , Dermatoses do Pé/microbiologia , Dermatoses do Pé/patologia , Pele/microbiologia , Pele/patologia , Spirochaetales/isolamento & purificação , Infecções por Spirochaetales/patologiaRESUMO
Treponemal infections in wild European brown hares (Lepus europaeus) from The Netherlands were diagnosed by means of serological tests for human syphilis and histological demonstration of spirochetes by the Bosma-Steiner silver impregnation method in histological sections of skin lesions. The treponeme should probably be classified as "Treponema paraluisleporis."
Assuntos
Animais Selvagens/microbiologia , Coelhos/microbiologia , Infecções por Treponema/veterinária , Animais , Masculino , Países Baixos , Pênis/microbiologia , Pênis/patologia , Pele/microbiologia , Pele/patologia , Sorodiagnóstico da Sífilis , Treponema/classificação , Treponema/isolamento & purificação , Infecções por Treponema/diagnóstico , Infecções por Treponema/microbiologiaAssuntos
Pele/microbiologia , Treponema/isolamento & purificação , Biópsia , Humanos , Pele/patologiaRESUMO
Spirochaetes were demonstrated in material from patients with Lyme disease by short-time high-concentrate silver impregnation after treatment with amylase. Removal of mucoid material was essential to visualise Borrelia burgdorferi. Lyme spirochaetes were demonstrated in material from 23 patients with Lyme disease--erythema chronicum migrans (ECM) 10, lymphadenosis benigna cutis (LABC) 7, arthritis 4 and Bannwarth's syndrome 2. Spirochaetes were localised in the subepidermal zone, peri- and intravascularly, and in collagen fibres in ECM and LABC, and beneath the synovial lining cells in arthritis, producing marked vascular changes with fibrosis and synovial hyperplasia. Spirochaetes were also demonstrated in CSF from a patient with Bannwarth's syndrome.