Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 8 de 8
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Ophthalmic Epidemiol ; 31(2): 99-106, 2024 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-37073137

RESUMO

PURPOSE: Reducing childhood blindness and vision impairment (BVI) remains a global health priority. Our purpose was to summarize the peer-reviewed literature to date on measuring and reporting childhood BVI using population-based surveys and vision examinations. METHODS: We conducted a scoping review of published studies that aimed to report BVI prevalence in children or studies that aimed to report BVI prevalence in the general population but which also included children. There were 201 articles identified for abstract review, and 86 studies were included in the final review. RESULTS: There were 52 studies (60%) that specifically aimed to investigate prevalence of blindness and/or vision impairment among child populations, while the remaining 34 studies aimed to study BVI in the general population but still reported data for age ranges that included children. The majority of researchers used the WHO criteria for blindness and vision impairment, sometimes with modifications. Age definitions for children varied considerably, with maximum cutoffs ranging from 3 to 20 years. CONCLUSION: The available literature on childhood blindness indicates that while there has been substantial progress towards establishing an evidence base, more remains to be accomplished in terms of addressing gaps in understanding of the true prevalence and impacts of childhood blindness and vision loss. All studies in this review cited the need for improved vision care services, either for all ages or for the childhood years in particular.


Assuntos
Cegueira , Baixa Visão , Criança , Humanos , Pré-Escolar , Adolescente , Adulto Jovem , Adulto , Acuidade Visual , Cegueira/epidemiologia , Baixa Visão/epidemiologia , Transtornos da Visão , Prevalência
3.
PLoS Genet ; 10(10): e1004604, 2014 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-25356765

RESUMO

The Second Heart Field (SHF) has been implicated in several forms of congenital heart disease (CHD), including atrioventricular septal defects (AVSDs). Identifying the SHF gene regulatory networks required for atrioventricular septation is therefore an essential goal for understanding the molecular basis of AVSDs. We defined a SHF Hedgehog-dependent gene regulatory network using whole genome transcriptional profiling and GLI-chromatin interaction studies. The Forkhead box transcription factors Foxf1a and Foxf2 were identified as SHF Hedgehog targets. Compound haploinsufficiency for Foxf1a and Foxf2 caused atrioventricular septal defects, demonstrating the biological relevance of this regulatory network. We identified a Foxf1a cis-regulatory element that bound the Hedgehog transcriptional regulators GLI1 and GLI3 and the T-box transcription factor TBX5 in vivo. GLI1 and TBX5 synergistically activated transcription from this cis-regulatory element in vitro. This enhancer drove reproducible expression in vivo in the posterior SHF, the only region where Gli1 and Tbx5 expression overlaps. Our findings implicate Foxf genes in atrioventricular septation, describe the molecular underpinnings of the genetic interaction between Hedgehog signaling and Tbx5, and establish a molecular model for the selection of the SHF gene regulatory network for cardiac septation.


Assuntos
Fatores de Transcrição Forkhead/genética , Defeitos dos Septos Cardíacos/genética , Coração/fisiopatologia , Proteínas com Domínio T/genética , Animais , Cromatina/genética , Regulação da Expressão Gênica no Desenvolvimento , Redes Reguladoras de Genes , Defeitos dos Septos Cardíacos/patologia , Proteínas Hedgehog/genética , Humanos , Fatores de Transcrição Kruppel-Like/genética , Camundongos , Proteínas do Tecido Nervoso/genética , Transdução de Sinais , Fatores de Transcrição/genética , Proteína GLI1 em Dedos de Zinco , Proteína Gli3 com Dedos de Zinco
4.
Hum Mol Genet ; 20(19): 3725-37, 2011 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-21653639

RESUMO

The primary cilium is emerging as a crucial regulator of signaling pathways central to vertebrate development and human disease. We identified atrioventricular canal 1 (avc1), a mouse mutation that caused VACTERL association with hydrocephalus, or VACTERL-H. We showed that avc1 is a hypomorphic mutation of intraflagellar transport protein 172 (Ift172), required for ciliogenesis and Hedgehog (Hh) signaling. Phenotypically, avc1 caused VACTERL-H but not abnormalities in left-right (L-R) axis formation. Avc1 resulted in structural cilia defects, including truncated cilia in vivo and in vitro. We observed a dose-dependent requirement for Ift172 in ciliogenesis using an allelic series generated with Ift172(avc1) and Ift172(wim), an Ift172 null allele: cilia were present on 42% of avc1 mouse embryonic fibroblast (MEF) and 28% of avc1/wim MEFs, in contrast to >90% of wild-type MEFs. Furthermore, quantitative cilium length analysis identified two specific cilium populations in mutant MEFS: a normal population with normal IFT and a truncated population, 50% of normal length, with disrupted IFT. Cells from wild-type embryos had predominantly full-length cilia, avc1 embryos, with Hh signaling abnormalities but not L-R abnormalities, had cilia equally divided between full-length and truncated, and avc1/wim embryos, with both Hh signaling and L-R abnormalities, were primarily truncated. Truncated Ift172 mutant cilia showed defects of the distal ciliary axoneme, including disrupted IFT88 localization and Hh-dependent Gli2 localization. We propose a model in which mutation of Ift172 results in a specific class of abnormal cilia, causing disrupted Hh signaling while maintaining L-R axis determination, and resulting in the VACTERL-H phenotype.


Assuntos
Cardiopatias Congênitas/genética , Hidrocefalia/genética , Peptídeos e Proteínas de Sinalização Intracelular/genética , Deformidades Congênitas dos Membros/genética , Camundongos/genética , Proteínas Adaptadoras de Transdução de Sinal , Alelos , Canal Anal/anormalidades , Canal Anal/embriologia , Canal Anal/metabolismo , Animais , Cílios/genética , Cílios/metabolismo , Proteínas do Citoesqueleto , Modelos Animais de Doenças , Esôfago/anormalidades , Esôfago/embriologia , Esôfago/metabolismo , Cardiopatias Congênitas/embriologia , Cardiopatias Congênitas/metabolismo , Proteínas Hedgehog/genética , Proteínas Hedgehog/metabolismo , Humanos , Hidrocefalia/embriologia , Hidrocefalia/metabolismo , Peptídeos e Proteínas de Sinalização Intracelular/metabolismo , Rim/anormalidades , Rim/embriologia , Rim/metabolismo , Deformidades Congênitas dos Membros/embriologia , Deformidades Congênitas dos Membros/metabolismo , Camundongos/metabolismo , Camundongos Endogâmicos C3H , Camundongos Endogâmicos C57BL , Mutagênese , Mutação , Transporte Proteico , Transdução de Sinais/genética , Coluna Vertebral/anormalidades , Coluna Vertebral/embriologia , Coluna Vertebral/metabolismo , Traqueia/anormalidades , Traqueia/embriologia , Traqueia/metabolismo , Proteínas Supressoras de Tumor/genética , Proteínas Supressoras de Tumor/metabolismo
5.
Breast Cancer Res Treat ; 122(1): 159-68, 2010 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-19777343

RESUMO

The small heat shock protein alphaB-crystallin is a molecular chaperone that is induced by stress and protects cells by inhibiting protein aggregation and apoptosis. To identify novel transcriptional regulators of the alphaB-crystallin gene, we examined the alphaB-crystallin promoter for conserved transcription factor DNA-binding elements and identified a putative response element for the p53 tumor suppressor protein. Ectopic expression of wild-type p53 induced alphaB-crystallin mRNA and protein with delayed kinetics compared to p21. Additionally, the induction of alphaB-crystallin by genotoxic stress was inhibited by siRNAs targeting p53. Although the p53-dependent transactivation of an alphaB-crystallin promoter luciferase reporter required the putative p53RE, chromatin immunoprecipitation failed to detect p53 binding to the alphaB-crystallin promoter. These results suggested an indirect mechanism of transactivation involving p53 family members p63 or p73. DeltaNp73 was dramatically induced by p53 in a TAp73-dependent manner, and silencing p73 suppressed the transcriptional activation of alphaB-crystallin by p53. Moreover, ectopic expression of DeltaNp73alpha (but not other p73 isoforms) increased alphaB-crystallin mRNA levels in the absence of p53. Collectively, our results link the molecular chaperone alphaB-crystallin to the cellular genotoxic stress response via a novel mechanism of transcriptional regulation by p53 and p73.


Assuntos
Dano ao DNA/fisiologia , Proteínas de Ligação a DNA/fisiologia , Regulação Neoplásica da Expressão Gênica/genética , Proteínas Nucleares/fisiologia , Proteína Supressora de Tumor p53/fisiologia , Proteínas Supressoras de Tumor/fisiologia , Cadeia B de alfa-Cristalina/genética , Sítios de Ligação , Carcinoma de Células de Transição/patologia , Linhagem Celular Tumoral/metabolismo , Doxorrubicina/toxicidade , Genes Reporter , Proteínas de Choque Térmico HSP27/biossíntese , Proteínas de Choque Térmico HSP27/genética , Humanos , Mutagênese Sítio-Dirigida , Proteínas de Neoplasias/biossíntese , Proteínas de Neoplasias/genética , Regiões Promotoras Genéticas/genética , Isoformas de Proteínas/fisiologia , Proteínas Recombinantes de Fusão/fisiologia , Alinhamento de Sequência , Ativação Transcricional , Proteína Tumoral p73 , Neoplasias da Bexiga Urinária/patologia , Cadeia B de alfa-Cristalina/biossíntese
6.
Breast Cancer Res Treat ; 119(1): 63-70, 2010 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-19205872

RESUMO

Recent studies indicate that the small heat shock protein alphaB-crystallin is expressed in poor prognosis basal-like breast tumors and likely contributes to their aggressive phenotype. However, the mechanisms underlying the deregulated expression of alphaB-crystallin in basal-like tumors are poorly understood. Using a bioinformatics approach, we identified a putative DNA binding motif in the human alphaB-crystallin promoter for the proto-oncogene Ets1, a member of the ETS transcription factor family that bind to DNA at palindromic ETS-binding sites (EBS). Here we demonstrate that ectopic expression of Ets1 activates the alphaB-crystallin promoter by an EBS-dependent mechanism and increases alphaB-crystallin protein levels, while silencing Ets1 reduces alphaB-crystallin promoter activity and protein levels. Chromatin immunoprecipitation analyses showed that endogenous Ets1 binds to the alphaB-crystallin promoter in basal-like breast cancer cells in vivo. Interrogation of publically available gene expression data revealed that Ets1 is expressed in human basal-like breast tumors and is associated with poor survival. Collectively, our results point to a previously unrecognized link between the oncogenic transcription factor Ets1 and alphaB-crystallin in basal-like breast cancer.


Assuntos
Neoplasias da Mama/metabolismo , Regulação Neoplásica da Expressão Gênica , Proteína Proto-Oncogênica c-ets-1/biossíntese , Cadeia B de alfa-Cristalina/biossíntese , Animais , Sequência de Bases , Linhagem Celular Tumoral , Imunoprecipitação da Cromatina , Biologia Computacional/métodos , Feminino , Inativação Gênica , Humanos , Chaperonas Moleculares/metabolismo , Dados de Sequência Molecular , Regiões Promotoras Genéticas , Proto-Oncogene Mas
7.
Clin Cancer Res ; 14(10): 3168-76, 2008 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-18483385

RESUMO

PURPOSE: Although tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) and agonistic antibodies targeting its receptors are promising cancer therapies because of their tumor selectivity, many tumors are resistant to TRAIL-based therapies. We examined whether the nonsteroidal anti-inflammatory drug aspirin sensitized cancer cells to TRAIL agonists in vitro and in vivo and investigated the underlying mechanism. EXPERIMENTAL DESIGN: The effects of aspirin on sensitivity to TRAIL agonists and expression of apoptosis regulators was determined in human breast cancer cell lines and xenograft tumors. The specific role of survivin depletion in the TRAIL-sensitizing effects of aspirin was determined by silencing survivin. RESULTS: Aspirin sensitized human breast cancer cells, but not untransformed human mammary epithelial cells, to TRAIL-induced caspase activation and apoptosis by a cyclooxygenase-2-independent mechanism. Aspirin also sensitized breast cancer cells to apoptosis induced by a human agonistic TRAIL receptor-2 monoclonal antibody (lexatumumab). Aspirin treatment led to G1 cell cycle arrest and a robust reduction in the levels of the antiapoptotic protein survivin by inducing its proteasomal degradation, but did not affect the levels of many other apoptosis regulators. Silencing survivin with small interfering RNAs sensitized breast cancer cells to TRAIL-induced apoptosis, underscoring the functional role of survivin depletion in the TRAIL-sensitizing actions of aspirin. Moreover, aspirin acted synergistically with TRAIL to promote apoptosis and reduce tumor burden in an orthotopic breast cancer xenograft model. CONCLUSIONS: Aspirin sensitizes transformed breast epithelial cells to TRAIL-based therapies in vitro and in vivo by a novel mechanism involving survivin depletion. These findings provide the first in vivo evidence for the therapeutic utility of this combination.


Assuntos
Protocolos de Quimioterapia Combinada Antineoplásica/farmacologia , Apoptose/efeitos dos fármacos , Aspirina/farmacologia , Neoplasias da Mama/tratamento farmacológico , Proteínas Associadas aos Microtúbulos/efeitos dos fármacos , Proteínas de Neoplasias/efeitos dos fármacos , Ligante Indutor de Apoptose Relacionado a TNF/farmacologia , Animais , Anti-Inflamatórios não Esteroides/farmacologia , Anticorpos Monoclonais/farmacologia , Anticorpos Monoclonais Humanizados , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Sinergismo Farmacológico , Feminino , Expressão Gênica/efeitos dos fármacos , Humanos , Immunoblotting , Marcação In Situ das Extremidades Cortadas , Proteínas Inibidoras de Apoptose , Camundongos , Camundongos Nus , Proteínas Associadas aos Microtúbulos/biossíntese , Proteínas de Neoplasias/biossíntese , Receptores do Ligante Indutor de Apoptose Relacionado a TNF/efeitos dos fármacos , Receptores do Ligante Indutor de Apoptose Relacionado a TNF/metabolismo , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Survivina , Ensaios Antitumorais Modelo de Xenoenxerto
8.
J Biol Chem ; 279(53): 56053-60, 2004 Dec 31.
Artigo em Inglês | MEDLINE | ID: mdl-15509580

RESUMO

Alterations in protein folding and the regulation of conformational states have become increasingly important to the functionality of key molecules in signaling, cell growth, and cell death. Molecular chaperones, because of their properties in protein quality control, afford conformational flexibility to proteins and serve to integrate stress-signaling events that influence aging and a range of diseases including cancer, cystic fibrosis, amyloidoses, and neurodegenerative diseases. We describe here characteristics of celastrol, a quinone methide triterpene and an active component from Chinese herbal medicine identified in a screen of bioactive small molecules that activates the human heat shock response. From a structure/function examination, the celastrol structure is remarkably specific and activates heat shock transcription factor 1 (HSF1) with kinetics similar to those of heat stress, as determined by the induction of HSF1 DNA binding, hyperphosphorylation of HSF1, and expression of chaperone genes. Celastrol can activate heat shock gene transcription synergistically with other stresses and exhibits cytoprotection against subsequent exposures to other forms of lethal cell stress. These results suggest that celastrols exhibit promise as a new class of pharmacologically active regulators of the heat shock response.


Assuntos
Resposta ao Choque Térmico , Triterpenos/farmacologia , Western Blotting , Linhagem Celular , Proliferação de Células , Cromatina/metabolismo , DNA/química , Proteínas de Ligação a DNA/química , Relação Dose-Resposta a Droga , Células HeLa , Fatores de Transcrição de Choque Térmico , Humanos , Imunoprecipitação , Cinética , Luciferases/metabolismo , Modelos Químicos , Triterpenos Pentacíclicos , Fosforilação , Plasmídeos/metabolismo , Reação em Cadeia da Polimerase , Conformação Proteica , Dobramento de Proteína , Quinonas/química , Transdução de Sinais , Relação Estrutura-Atividade , Temperatura , Fatores de Tempo , Fatores de Transcrição , Transfecção , Triterpenos/química
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...