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1.
Vet Res ; 54(1): 31, 2023 Apr 04.
Artigo em Inglês | MEDLINE | ID: mdl-37016427

RESUMO

The available differentiating tests for Chlamydia are based on detection of genetic material and only give information about the actual infection status, but reveal nothing of past infections. As the use of serological methods increases the window of detection, the goal of this study was to investigate if it is possible to develop a differentiating serological test for antibodies against Chlamydia species in chicken sera. Focus was on C. psittaci, C. gallinacea, and two closely related species, i.e. C. abortus and C. avium. To enable differentiating serology, a bead-based Luminex suspension array was constructed, using peptides as antigens, derived from known immunoreactive Chlamydia proteins. For the majority of these peptides, species-specific seroreactivity in mammalian sera has been reported in literature. The suspension array correctly identified antibodies against various Chlamydia species in sera from experimentally infected mice, and was also able to differentiate between antibodies against C. psittaci and C. gallinacea in sera from experimentally infected chickens. In field sera, signals were difficult to interpret as insufficient sera from experimentally infected chickens were available for evaluating the seroreactivity of all peptides. Nevertheless, results of the suspension array with field sera are supported by published data on the occurrence of C. gallinacea in Dutch layers, thereby demonstrating the proof of concept of multiplex serology for Chlamydial species in poultry.


Assuntos
Anticorpos Antibacterianos , Antígenos de Bactérias , Técnicas Bacteriológicas , Infecções por Chlamydia , Peptídeos , Animais , Camundongos , Anticorpos Antibacterianos/sangue , Antígenos de Bactérias/química , Antígenos de Bactérias/metabolismo , Galinhas , Chlamydia , Infecções por Chlamydia/diagnóstico , Infecções por Chlamydia/veterinária , Peptídeos/química , Peptídeos/metabolismo , Técnicas Bacteriológicas/métodos , Técnicas Bacteriológicas/veterinária
2.
Virulence ; 10(1): 334-351, 2019 12.
Artigo em Inglês | MEDLINE | ID: mdl-30957693

RESUMO

Streptococcus suis is a Gram-positive bacterium and a zoonotic pathogen residing in the nasopharynx or the gastrointestinal tract of pigs with a potential of causing life-threatening invasive disease. It is endemic in the porcine production industry worldwide, and it is also an emerging human pathogen. After invasion, the pathogen adapts to cause bacteremia and disseminates to different organs including the brain. To gain insights in this process, we infected piglets with a highly virulent strain of S. suis, and bacterial transcriptomes were obtained from blood and different organs (brain, joints, and heart) when animals had severe clinical symptoms of infection. Microarrays were used to determine the genome-wide transcriptional profile at different infection sites and during growth in standard growth medium in vitro. We observed differential expression of around 30% of the Open Reading Frames (ORFs) and infection-site specific patterns of gene expression. Genes with major changes in expression were involved in transcriptional regulation, metabolism, nutrient acquisition, stress defenses, and virulence, amongst others, and results were confirmed for a subset of selected genes using RT-qPCR. Mutants were generated in two selected genes, and the encoded proteins, i.e., NADH oxidase and MetQ, were shown to be important virulence factors in coinfection experiments and in vitro assays. The knowledge derived from this study regarding S. suis gene expression in vivo and identification of virulence factors is important for the development of novel diagnostic and therapeutic strategies to control S. suis disease.


Assuntos
Adaptação Fisiológica/genética , Infecções Estreptocócicas/veterinária , Streptococcus suis/genética , Streptococcus suis/patogenicidade , Transcriptoma , Fatores de Virulência/genética , Transportadores de Cassetes de Ligação de ATP/genética , Transportadores de Cassetes de Ligação de ATP/metabolismo , Animais , Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , Análise em Microsséries , Complexos Multienzimáticos/genética , Complexos Multienzimáticos/metabolismo , Mutação , NADH NADPH Oxirredutases/genética , NADH NADPH Oxirredutases/metabolismo , Infecções Estreptocócicas/microbiologia , Suínos , Doenças dos Suínos/microbiologia , Fatores de Virulência/metabolismo
3.
Cell Microbiol ; 14(8): 1287-98, 2012 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-22524898

RESUMO

Mycobacterium species, including Mycobacterium tuberculosis and Mycobacterium leprae, are among the most potent human bacterial pathogens. The discovery of cytosolic mycobacteria challenged the paradigm that these pathogens exclusively localize within the phagosome of host cells. As yet the biological relevance of mycobacterial translocation to the cytosol remained unclear. In this current study we used electron microscopy techniques to establish a clear link between translocation and mycobacterial virulence. Pathogenic, patient-derived mycobacteria species were found to translocate to the cytosol, while non-pathogenic species did not. We were further able to link cytosolic translocation with pathogenicity by introducing the ESX-1 (type VII) secretion system into the non-virulent, exclusively phagolysosomal Mycobacterium bovis BCG. Furthermore, we show that translocation is dependent on the C-terminus of the early-secreted antigen ESAT-6. The C-terminal truncation of ESAT-6 was shown to result in attenuation in mice, again linking translocation to virulence. Together, these data demonstrate the molecular mechanism facilitating translocation of mycobacteria. The ability to translocate from the phagolysosome to the cytosol is with this study proven to be biologically significant as it determines mycobacterial virulence.


Assuntos
Citoplasma/microbiologia , Mycobacterium/patogenicidade , Antígenos de Bactérias/química , Antígenos de Bactérias/metabolismo , Proteínas de Bactérias/química , Proteínas de Bactérias/metabolismo , Linhagem Celular , Técnicas de Introdução de Genes , Interações Hospedeiro-Patógeno , Humanos , Lisossomos/microbiologia , Lisossomos/ultraestrutura , Mycobacterium/genética , Mycobacterium/metabolismo , Fagossomos/microbiologia , Fagossomos/ultraestrutura , Estrutura Terciária de Proteína , Ubiquitina/metabolismo , Fatores de Virulência/genética , Fatores de Virulência/metabolismo
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