RESUMO
The process of collagen and proteoglycan synthesis in the in vitro cultured fibroblasts was studied by four original histoenzymological methods: UDP-glucuronic-5-epimerase, hydroxyproline epimerase, UDP-4-epimerase and UDP-N-acetyl-glucosamine epimerase. The process of epimerasization varied in terms of the cell growth stages. Synthesis of collagen and proteoglycans developed in the cells of the same culture but with various intensities in different cell groups.
Assuntos
Tecido Conjuntivo/enzimologia , Fibroblastos/enzimologia , Isomerases/análise , Isomerases/metabolismo , Racemases e Epimerases/análise , Racemases e Epimerases/metabolismo , Animais , Células Cultivadas/enzimologia , Embrião de Galinha , Colágeno/biossíntese , Fibroblastos/metabolismo , Fibroblastos/ultraestrutura , Proteoglicanas/biossínteseRESUMO
The activity of dihydrofolate dehydrogenase by histochemical method in children leukocytes was investigated. Blood leukocytes have a positive enzymic activity. Except monocytes a large enzymic variability was observed in neutrophils, eosinophils, and lymphocytes in puberty and at the onset of the adolescence. Enzymic activity varies with blood cellular type, the age, in males and females and from one child to another.
