RESUMO
Unclassified virus named Komandory virus (KOMV) isolated in Komandorski islands from the ticks Ixodes uriae in 1986 was partially sequenced. The KOMV nucleocapsid (N) protein sequence shows 30-40% identity level with the mosquito-borne phleboviruses and 43% with the Uukuniemi virus (UUKV) group (Phlebovirus, Bunyaviridae). The maximum identity (65%) for the KOMV N protein is observed for the Manawa virus. The KOMV glycoprotein identity with the UUKV group viruses ranges from 45% to 59%. The KOMV RdRp identity with the Manawa virus reaches 74%, while showing 63% level at average with the other UUKV group viruses. According to the results of molecular-genetic and phylogenetic analysis, the KOMV is a new member of the UUKV group (Phlebovirus, Bunyaviridae).
Assuntos
Charadriiformes/virologia , Genoma Viral , Ixodes/virologia , Proteínas do Nucleocapsídeo/classificação , Phlebovirus/classificação , Filogenia , DNA Polimerase Dirigida por RNA/classificação , Sequência de Aminoácidos , Animais , Ilhas , Dados de Sequência Molecular , Comportamento de Nidação , Proteínas do Nucleocapsídeo/genética , Phlebovirus/genética , Phlebovirus/isolamento & purificação , DNA Polimerase Dirigida por RNA/genética , Federação Russa , Alinhamento de Sequência , Homologia de Sequência de AminoácidosRESUMO
The Baku virus (BAKV) was originally isolated from the ticks Ornithodoros capensis Neumann, 1901 (Acari: Argasidae) collected from the seagull (Larus argentatus) seating nests on the islands of the Baku archipelago, the Caspian sea. BAKV was assigned to Kemerovo group (KEMV) (Orbivirus, Reoviridae). The BAKV was frequently isolated from the ticks O. coniceps Canestrini, 1980, collected from L. argentatus and tern (Sterna hirundo) nests in Turkmenia and pigeon (Columba livia neglecta) nests in Uzbekistan. In this work, the genome of the BAKV was sequenced using the next-generation sequencing technology. The BAKV Pol protein has 48.6% identity level with the viruses of the Great Island Virus group and at average 41% with non-tick orbiviruses. The BAKV T2 protein level identity with the orbiviruses ranges from 23.7% to 64.8%. The maximum identity level of the T2 protein (64.8%) is observed for the tick-borne viruses of the GIV (KEMV) group. According to the conducted molecular-genetic and phylogenetic analysis, the BAKV is a novel species of the genus Orbivirus. It forms a phylogenetic group distinctly related to the GIV group.
