RESUMO
Cell transformation induced by murine sarcoma virus (MSV-M) is significantly inhibited (80--90%) in a clonal line of normal rat kidney (NRK) cells when they are infected with rat cell passaged adenovirus 12 (R-Ad12). No inhibition is seen when R-Ad12 is added simultaneously with, or 1 1/2 or 24 hr after, MSV-M infection, suggesting that inhibition occurs most probably intracellularly. There is also a direct correlation between the extent of focus formation and the concentration of R-Ad12 used. Concentrations of R-Ad12 used to inhibit cell transformation do not affect cell growth. Significant inhibition in foci (90--100%) is also evident in the first two subcultures of R-Ad12 infected NRK cells. The mechanism of the inhibition is not yet known.
Assuntos
Adenovírus Humanos/crescimento & desenvolvimento , Transformação Celular Neoplásica , Transformação Celular Viral , Vírus do Sarcoma Murino/crescimento & desenvolvimento , Adenovírus Humanos/imunologia , Animais , Antígenos Virais/análise , Linhagem Celular , Células Clonais , Efeito Citopatogênico Viral , Citoplasma/imunologia , Rim , Ratos , Replicação ViralRESUMO
Twenty-one cases of bacteremia due to Bifidobacterium, Eubacterium and Lactobacillus are described. Transient bacteremic episodes with these organisms may follow trauma to the mouth, intestine, or vagina. The majority of the patients were female and most had an underlying condition that may have predisposed to bacteremia. Ten of the patients died despite antibiotic treatment.
Assuntos
Actinomycetaceae , Eubacterium , Lactobacillus , Sepse/etiologia , Actinomycetaceae/isolamento & purificação , Adulto , Idoso , Eubacterium/isolamento & purificação , Feminino , Humanos , Lactobacillus/isolamento & purificação , Masculino , Pessoa de Meia-Idade , Gravidez , Complicações Infecciosas na Gravidez/etiologiaRESUMO
A variant of adenovirus type 12 (R-Ad12) has been isolated by infecting a clonal line of normal rat kidney cells (NRK) with wild-type adenovirus 12. Although R-Ad12 failed to produce infectious progeny virions when cycled a second time through NRK cells, it nevertheless retained the ability to code for virus structural proteins that accumulated in the cytoplasm.
Assuntos
Adenovírus Humanos/metabolismo , Capsídeo/metabolismo , Citoplasma/metabolismo , Proteínas Virais/metabolismo , Adenovírus Humanos/crescimento & desenvolvimento , Linhagem Celular , Células Clonais , DNA Viral , Ensaio de Placa ViralRESUMO
The multiplication rate of bacteria in undiluted blood containing sodium polyanethol sulfonate was compared with growth rates obtained in dilutions of blood ranging from 1:2 to 1:8. Although all organisms tested grew in the undiluted blood, increased growth rates were seen in the 1:2 dilution. Further dilution resulted in growth rates equivalent to that obtained with the 1:2 dilution. In view of these results, we question the present recommendations that blood be diluted 1:10 or 1:20.
