Assuntos
Aeromonas veronii , Fasciite Necrosante/tratamento farmacológico , Fasciite Necrosante/cirurgia , Infecções por Bactérias Gram-Negativas/tratamento farmacológico , Infecções por Bactérias Gram-Negativas/cirurgia , Aeromonas veronii/isolamento & purificação , Idoso , Antibacterianos/administração & dosagem , Cefepima/administração & dosagem , Ciprofloxacina/administração & dosagem , Terapia Combinada , Tratamento Conservador , Quimioterapia de Consolidação/efeitos adversos , Desbridamento , Quimioterapia Combinada , Fasciite Necrosante/microbiologia , Infecções por Bactérias Gram-Negativas/complicações , Humanos , Leucemia Mieloide Aguda/complicações , Leucemia Mieloide Aguda/tratamento farmacológico , Leucemia Mieloide Aguda/microbiologia , Masculino , Resultado do TratamentoRESUMO
Our investigations hereby involve the effects of ionizing radiation as a sterilization method, and especially electron beams, on a medical grade Polyethylene Terephthalate (PET). Ionizing radiations are known to induce free radicals formation in the polymer that will then either degrade or crosslink, depending on its chemical nature, ionizing type and irradiation atmosphere (Charlesby, 1967; Dole, 1972 and Dole, 1973). The evaluation of packaging material modifications under radiation sterilization is of great interest and the objective of our paper is to focus on both PET volume modifications and extractable analyses after electron beam sterilization. As regards the polymeric matrix and after sterilization, we did not observe by means of spectral analyses, any single chemical modification whatsoever, whereas by thermal techniques we evidenced polymer chain scissions. As for the non-volatile organic extractables, we further substantiate the presence of numerous compounds, namely oligomers and trimers especially, along with benzoic and terephthalic acids in particular. With respect to pharmaceutical stakes however, we claim herein that the 25kGy sterilization dose used, triggers oligomers and extractables formation, a result which is promising for further risk analysis.
Assuntos
Elétrons , Polietilenotereftalatos/química , Polietilenotereftalatos/efeitos da radiação , Esterilização/métodos , Compostos Orgânicos Voláteis/química , Compostos Orgânicos Voláteis/efeitos da radiação , Varredura Diferencial de Calorimetria , Cromatografia em Gel , Cromatografia Líquida de Alta Pressão , Reagentes de Ligações Cruzadas , Radicais Livres , Espectroscopia de Infravermelho com Transformada de Fourier , TermogravimetriaRESUMO
In neonatal intensive care units, topical agents represent an increasing part of the infection control armamentarium. Fifty-one coagulase-negative staphylococci (CNS) isolated from catheter-associated bloodstream infections in very preterm neonates were investigated in this study: 41.2% exhibited decreased susceptibility to at least one antiseptic (chlorhexidine 12%, benzalkonium 24%, acriflavine 33%) and 61% were resistant to mupirocin. QacA/B, mupA and both genes were detected by polymerase chain reaction in 59%, 63% and 49% of CNS, respectively. Seventy-six percent of Staphylococcus epidermidis (5/5 pulsed-field-gel electrophoresis subgroups) and 11% of Staphylococcus capitis (1/3 subgroups) were multi-resistant. Skin antisepsis using low-concentration aqueous formulations and off-label mupirocin indications should benefit from a stewardship programme.
