RESUMO
Postmortem changes in alcohol and drug concentrations are well known today. The authors used an HPLC assay of barbiturates in postmortem tissue, and developed a rat model in order to evidence postmortem changes in toxics concentrations. Postmortem changes in secobarbital concentrations were evidenced using a rat-secobarbital model. This work emphasizes the difficulty of postmortem toxicology, as concentrations found at the time of autopsy may be different from concentrations at the time of death.
Assuntos
Mudanças Depois da Morte , Secobarbital/análise , Animais , Cromatografia Líquida de Alta Pressão , Rim/química , Fígado/química , Miocárdio/química , Ratos , Ratos Wistar , Secobarbital/intoxicação , Baço/químicaRESUMO
Post-mortem changes in barbiturate concentrations were evidenced using a rat-secobarbital model. The method used for the extraction and HPLC assay of barbiturates is suitable for all biologic fluids and post-mortem tissues. Kinetic data obtained is of excellent quality. Various modelization constants were defined. This experimental work emphasizes the difficulty of post-mortem toxicology, as concentrations found at the time of autopsy may be different from concentrations at the time of death, in blood as well as in tissues.
Assuntos
Mudanças Depois da Morte , Secobarbital/farmacocinética , Animais , Cromatografia Líquida de Alta Pressão , Ratos , Ratos EndogâmicosAssuntos
Cromonas/farmacocinética , Dietilaminas/farmacocinética , Olho/metabolismo , Animais , Cromonas/administração & dosagem , Dietilaminas/administração & dosagem , Combinação de Medicamentos/administração & dosagem , Combinação de Medicamentos/farmacocinética , Soluções Oftálmicas , Coelhos , Distribuição TecidualRESUMO
After the debridement of the entire corneal epithelium of the rabbit eye, epithelial cells of conjunctival origin cover the denuded corneal surface. Under such experimental conditions, the rate of wound healing is considerably delayed and total regeneration is completed within 15 to 20 days, allowing evaluation of various drugs, such as the Fibroblast Growth Factor. Both acidic and basic FGF were administered topically on totally de-epithelialized rabbit eye, at three different concentrations of 1.5 and 10 Stimulation Units/50 microliters, 3 times per day. A dose-response effect was observed and in each case, acidic FGF was found to be much more potent than bFGF in increasing the rate of wound healing of the cornea. These results are correlated with a new purification procedure, avoiding acid treatment of the tissue extract. The systemic diffusion of FGF allows the contralateral eye cells to be also stimulated for mitosis and migration and to heal faster than the control eyes.
