[Functional state of the ileum, cationic peptids and zinc content in the Paneth cells of the rats following lincomycin, indomethacin and methotrexate andministration].

In the paper the relation between the changes of a functional condition of ileum, composition of its microflora and secretory activity of Paneth cells has been investigated. Administration of lincomycin led to a reduction of total of symbiotic bacteria and appearance of opportunistic microflora. Statistically insignificant changes of speed of transit of intestinal contents and damage of barrier function of an intestinal epithelium were noted. The intestinal epithelium was characterized by signs of inflammation. Administration of indomethacin and methotrexate led to significant changes of a functional state of a small intestine of rats. The speed of transit of intestinal contents following indomethacin administration increased by 30%, and, on the contrary, decreased by 90% following administration of methotrexate. The ability of epithelium of ileum to adsorb vital dye in both cases increased (by 32 and 51%, respectively) indicating the damage of barrier function. Thus there were significant changes in structure of microflora of ileum: reduction of quantity symbiotic and emergence of opportunistic microflora, a translocation of bacteria to a mucous wall. It was concluded that animal of all three experimental groups had a reduction of the contents of cationic proteins and zinc in the secretory granules of Paneth cells. These results can testify that balance changes between simbiotic and opportunistic microflora are a factor which has an impact on intestine damage and also causes activation of secretory function of Paneth cells.

Effect of chelating agents and stress factors on zinc content in paneth cells and prostate gland cells.

The effects of chelating agents dithizone and sodium diethyldithiocarbamate on zinc content in Paneth cells and prostate gland cells were studied on rats preliminary exposed to stress factors (exercise and immobilization). Dithizone induced irreversible triphasic and sodium diethyldithiocarbamate reversible biphasic changes in zinc metabolism in cells. Preliminary stress potentiated the damaging effect of dithizone.

[Zinc and the secretory material content in blood granulocytes and in basal parts of intestine crypts during stress].

Zinc and the secretory material content in blood granulocytes and the Paneth cells of the rats increased by single physical load and immobilization. On the contrary, multiple physical loads and immobilization decreased the concentration of these components in the cells. Positive correlation of changes in both cell types pointed for possible functional connection between them.

[Zinc and insulin level in islet cells under various functional state of insular apparatus].

The work is devoted to the study of zinc role in pancreas incretory function. Golden hamster and human pancreatic beta cells were investigated under varions functional state of insular apparatus. An increase of zinc and insulin content in islet beta cells was observed during inhibition of its' secretory activity and decrease of both components content in the cells occurred after intensifying of its' activity. Under pancreatic diabetes zinc and insulin quantities in beta cells were significantly less the norm. The results of comparative investigations confirm the thesis concerning this metal and hormone connection in beta-insulocytes.

[Changes of zinc content in blood and cells of various organs in stress].

It was shown in experiments on rabbit and mice that acute stress induced increase in glycemia and zinc content of blood granulocytes, hippocampus, beta-insulocytes, the cells of intestinal krypts basal departments and prostate terminal departments. Such changes also occurred under adrenaline and prednisolone injections. On the contrary, the decrease in glycemia and cell zinc content is induced by pilocarpine injections. The data obtained indicate the role of sympato-adrenale system in regulation of cell zinc metabolism. The increase in cell zinc content under acute stress is accompanied by decrease of given metal concentration in blood plasma. This situation is evidently connected with zinc dislocation from the plasma into the cells. Zinc content changes in the cells of experimental animals were in conformity with their secretory material quantity changes, indicating possible functional connections of both components in the cells.

[Study on the connection of alcohol motivation with zinc content changes in the hippocampus]. / Vyvchennia zv'iazku alkohol'noï motyvatsiï zi zminamy vmistu tsynku v hipokampi.

Alcohol dependence development is accompanied by zinc content decrease in the hippocampus. Young age, repeated physical loads and immobilization are factors, that promote such changes development in more expressed degree.

[The effect of physiological and extreme irritants on zinc metabolism in pancreatic islets and hippocampus cells]. / Vmist tsynku v pankreatychnykh ostrivtsiakh i hipokampi pry diï fiziolohichnykh i nadzvychainykh podraznykiv.

Zinc content in pancreatic islet cells and hippocampus was studied with the aid of highly sensitive selective cytochemical reaction of 8-(p-toluensulfonylamino)-quinoline. It was shown that the factors stimulating B-insulocyte secretory activity induced the decrease of this metal concentrations in the cell. An increase of these cells zinc content observed in the cases of their secretory activity inhibition. The extreme irritants induced two phase changes of zinc concentrations in pancreatic islets and hippocampus.

[The cytochemical dithizone reaction of the blood granulocytes in zinc-deficient states]. / Tsytokhimichna dityzonova reaktsiia u hranulotsytakh krovi pry tsinkdefitsytnykh stanakh.

The blood granulocyte zinc content is liable to be reduced in hepatocirrhosis, chronic glomerulonephritis presenting with nephrotic syndrome, chronic enteritis, diabetes mellitus, and in frequent and excessive sweating, as evidence by a study conducted with the aid of a cytochemical dithizone test. A short discussion is given on potentials for application of the cytochemical dithizone test as a diagnostic aid to the study of the blood granulocyte zinc content in zinc deficiency states.

[The effect of immunosuppressive substances on the zinc content in cells]. / Vliianie immunosupressivnykh veshchestv na soderzhanie tsinka v kletkakh.

Decreases in zinc levels in the cells of various organs were observed in mice received cyclosporin A and sodium diethyldithiocarbamate. Glucocorticoid therapy induced a decrease of zinc levels in the granulocytes of human peripheral blood. The role of alterations in cell zinc metabolism in the mechanism of action of immunosuppressive agents is discussed in the paper.

[Epileptiform seizures induced by reagents selective for zinc]. / Epileptiformnye sudorogi, vyzyvaemye selektivnymi reagentami na tsink.

The administration of reagents selectively responding to zinc to dogs, cats, rabbits, golden hamsters and mice induced epileptoid seizures. The convulsive action of these agents was due to zinc binding in the hippocampus.

[Diagnostic value of selective cytochemical reaction to zinc in peripheral blood granulocytes]. / Diagnosticheskoe znachenie selektivnoi tsitokhimicheskoi reaktsii na tsink v granulotsitakh perifericheskoi krovi.

A method for cytochemical detection of zinc in peripheral blood granulocytes with selective reaction of 8-(p-toluenesulfonyl amino)-quinoline was developed. Zinc levels in granular leukocytes were found reduced in chronic myeloleukemia and elevated in leukemoid reactions. The method may be used for the differential diagnosis of these diseases.

[The significance of cytochemical studies of the metals in blood neutrophilic granulocytes in the diagnosis of lung diseases]. / Znachenie tsitokhimicheskikh issledovanii metallov v neitrofil'nykh granulotsitakh krovi pri diagnostike zabolevanii legkikh.

Peripheral blood smears from healthy persons and patients with pneumonia and pulmonary tuberculosis were used for a cytochemical study of metals in neutrophils with the help of dithisone and chlortetracycline. The content of metals in these cells was increased in pneumonia and active tuberculosis, but was normal or decreased in non-active pulmonary tuberculosis. It is concluded that cytochemical reactions for metals may be used in the diagnosis of pulmonary diseases.

[The effect of cyclosporin A on the islet cells of mice]. / Vliianie tsiklosporina A na insuliarnyi apparat myshei.

The action of immunosuppressive agent ciclosporin A on the insular apparatus was studied with an original method of cytochemical zinc detection in experiments on mice. The drug was found to lower zinc levels in pancreatic beta-cells. In ciclosporin A pretreatment the sensitivity of mice to zinc-binding diabetogenic agent enhanced.

[Zinc contents of pancreatic islets in animals of various species after administration of diabetogenic agent, dithizone]. / Soderzhanie tsinka v pankreaticheskikh ostrovkakh u zhivotnykh raznykh vidov pri vvedenenii diabetogennogo agenta ditizona.

A decrease of zinc content in pancreatic islet was shown in animals which received diabetogenic agent dithizone. The changes of islet zinc content occurred mainly on account of insulin-producing cells and in coincidence with glycemia changes.

[Zinc content of pancreatic islets in diabetes]. / Soderzhanie tsinka v pankreaticheskikh ostrovkakh pri diabete.

Content and distribution of zinc was studied in the pancreatic islets in human and rabbit diabetes. The diabetes development is followed by a decrease of zinc content in the insulin-producing cells in parallel with the severity of diabetes. The damage to the islet B-cells is the cause of zinc metabolism disturbance in the endocrine pancreas. Influence of high glucose concentration in the blood may be another factor.

[The dynamics of the Zn content in the pancreatic islets of rabbits after the administration of the diabetogenic agent dithizone]. / Dinamika soderzhaniia Zn v pankreaticheskikh ostrovkakh krolikov posle vvedeniia diabetogennogo agenta ditizona.

The dynamics of changes of the Zn content in the pancreatic islets of rabbits after dithizon injection was studied. The amount of this metal in the islets reduced due to destruction of the cells and under the effect of high blood plasma glucose concentration. The insulin producing cells were devoid of zinc in diabetes of long duration with high hyperglycemia.

[The effect of diabetogenic substances on the zinc and calcium content in the pancreatic islet cells of rabbits]. / Vliianie diabetogennykh veshchestv na soderzhanie tsinka i kal'tsiia v kletkakh pankreaticheskikh ostrovkov u krolikov.

The injections of diabetogenic drugs to rabbits induced a decrease in zinc and calcium content in B insulocytes dependent on the degree of these cells damage and hyperglycemia. The content of these metals in A insulocytes was not significantly altered due to a light injury of these cells. A high concentration of calcium in islet A cells is suggested to be a protective factor to the action of alteration agents.

The diabetogenic and acidotropic effects of chelators.

Diabetogenic and acidotropic effects of dithizone, 8-(p-toluenesulfonylamino)-quinoline and 8-(benzenesulfonylamino)-quinoline were studied in experiments on cats, rabbits, golden hamsters and mice. Selective damage of insulin producing cells, phase glycemic fluctuations and permanent diabetes development were shown to be connected with chelator zinc binding in the lysosome-segregation apparatus of these cells.

[Effect of fasting, glucose load and dithizone injections on the zinc content in pancreatic islets of the rabbit]. / Vliianie golodaniia, nagruzki gliukozoi i vvedeniia ditizona na soderzhanie tsinka v pankreaticheskikh ostrovkakh u krolikov.

The content of zinc in the pancreatic islets was increased in fasting animals and reduced after a glucose load and dithizone injection. A negative correlation between the blood sugar level and the zinc content in the pancreatic islets was encountered. A changed content of zinc in the islet cells may serve as a sensitive indicator of changes in the functional condition of the pancreatic insular apparatus.