RESUMO
We studied the potency of fortified ophthalmic antibiotic preparations of cefazolin sodium (50 mg/ml) and tobramycin sulfate (15 mg/ml), as measured by the minimum inhibitory concentration, against Streptococcus pneumoniae and Pseudomonas aeruginosa, respectively. We also examined absorbance spectra, pH, and the effect of storage temperature on these fortified solutions to determine their stability over a four-week period. Cefazolin and tobramycin maintained a constant potency throughout the experiment. There was no difference in potency if the fortified solutions were stored at 4 C or 24 C. Cefazolin stored at 24 C exhibited changes in both its absorbance spectra and pH after seven days. Cefazolin stored at 4 C and tobramycin stored at 24 C and 4 C remained stable throughout the four-week period.
Assuntos
Antibacterianos/farmacologia , Soluções Oftálmicas , Antibacterianos/administração & dosagem , Cefazolina/administração & dosagem , Cefazolina/farmacologia , Estabilidade de Medicamentos , Armazenamento de Medicamentos , Pseudomonas aeruginosa/efeitos dos fármacos , Streptococcus pneumoniae/efeitos dos fármacos , Temperatura , Fatores de Tempo , Tobramicina/administração & dosagem , Tobramicina/farmacologiaRESUMO
Experimental lens-induced uveitis was produced in Lewis rats by sensitization with bovine lens protein prior to disruption of the lens capsule. Treatment of these animals by intraperitoneal injection of dimethyl sulfoxide resulted in reduction in retinal vasculitis, hemorrhage and necrosis. Furthermore, morphometric analysis of choroidal inflammation revealed significant reduction of choroidal thickness in the treated animals. These findings suggest that hydroxyl radicals play a role in producing ocular tissue damage in the acute Arthus-type of ocular inflammation.
