RESUMO
The purpose of this study was to examine the effects of a physical activity intervention package on the self-esteem of pre-adolescent and adolescent females. The package involved three components: physical activity, education and self-report. Subjects (N = 181) were pre-, early-, and middle-adolescent girls ranging in age from 9 to 16 who were enrolled in an independent school. An experimental pre-test/post-test design which involved two independent variables (intervention package and age group), each with three levels, was used. Self-esteem was measured with the Self Description Questionnaire I and II (Marsh, 1988). Results indicated that low self-esteem individuals benefitted from the intervention. However, statistically significant results were limited to the younger age group.
Assuntos
Comportamento do Adolescente/psicologia , Comportamento Infantil/psicologia , Exercício Físico , Autoimagem , Adolescente , Criança , Feminino , HumanosRESUMO
The prototype strain of Flanders virus (61-7484) has been adapted to grow in Vero cell cultures and a plaque assay has been developed. Electron micrographs of infected cells revealed rod-shaped virions budding in clusters from the plasma membrane, consistent with the tentative classification of Flanders virus as a member of the Rhabdoviridae. Viral proteins were identified by SDS-PAGE and immunological techniques using Flanders virus-specific antibodies. Eight virus-associated proteins were detected. The relative molecular weights obtained by SDS-PAGE were 180,000, 81,000, 54,000, 43,000, 33,000, 27,000, 23,000, and 19,000. Evidence is presented that two of these proteins (43,000 and 23,000 mol wt) are in vivo cleavage products. The 81,000 mol wt protein was glycosylated and phosphorylated and did not label well with [35S]methionine or 14C-amino acids. The 54,000 and 33,000 mol wt proteins were phosphorylated. When 35S-radiolabeled virus was purified by pelleting at 90,000 g followed by isopycnic sucrose gradient centrifugation, all eight virus proteins were present in the peak fractions which contained the radiolabel and infectivity.
Assuntos
Rhabdoviridae/fisiologia , Proteínas Virais/biossíntese , Replicação Viral , Animais , Processamento de Proteína Pós-Traducional , Rhabdoviridae/classificação , Células Vero , Proteínas Virais/isolamento & purificaçãoAssuntos
Arbovírus/isolamento & purificação , Animais , Culicidae/microbiologia , Feminino , Camundongos , TexasRESUMO
The antigenic relationships among Hart Park (HP) virus strain AR 70, Flanders virus strain 61-7484, and other related strains were investigated. Strains AR 70 and 61-7484 were shown to be antigenically related but different from each other in neutralization, complement fixation, and double-diffusion tests. Two strains isolated in Texas and one strain each, isolated in Illinois and Florida, appeared to be closely related if not identical to Flanders virus (61-7484) and antigenically related but different from HP virus AR 70. Ten additional strains isolated in 10 different states were compared to the two prototype strains. Seven of the 10 could not be distinguished from Flanders virus, and all 10 strains were different from HP virus. The results indicate that a virus-type consisting of viruses antigenically related if not identical to Flanders virus is widely distributed within the United States, whereas HP virus is antigenically related but different from this type.
