[The structure and composition of the complex that provides the adhesion of the cellular layers during the morphogenesis of the human embryonic adenohypophysis]. / Struktura i sostav kompleksa, obespechivaiushchego adgeziiu kletochnykh plastov v khode morfogeneza adenogipofiza zarodyshei cheloveka.

The area of contact between adenohypophysis and diencephalon rudiments of human embryos (5-7 weeks of development) was studied using immunohistochemistry and electron microscopy. Basement membranes of Rathke's pouch ectoderm and of diencephalon bottom neuroectoderm are connected by means of a complex consisting of thin fibrous material and collagen-like fibrils. After Ca+2 and Mg+2 ions removal, this complex with basement membranes was separated from epithelial layers. The material in the contact area differed in its composition from the basement membranes covering the adenohypophysis and diencephalon rudiments by the high content of tenascin and the absence of EDB-fibronectin. Other basement membrane components (collagen IV, heparan-sulphate proteoglycans, entactin and laminin) were also present in this area. Tenascin accumulation was also found in Rathke's pouch epithelium and cavity.

[Local expression of cytokeratins 8, 17 and 18 in the mesenchyme and smooth muscles in the early stages of human organogenesis]. / Lokal'naia ékspressiia tsitokeratinov 8, 17 i 18 v mezenkhime i gladkikh myshtsakh na rannikh étapakh organogeneza cheloveka.

Expression of cytokeratins 7, 8, 17, 18 in human embryos and fetuses of 6.5-13 weeks was studied using light and electron immunocytochemistry and immunoelectroblotting with the monoclonal antibodies. Cytokeratins 8 and 18 were expressed in 6.5-8 week old embryos not only in epithelium but also in mesenchyme of allantois, urogenital sinus, Wolffian and Mullerian ducts, mesentery, urinary bladder and certain regions of colon, rectum and atrium cordis walls. Furthermore, starting from the 10th week smooth-muscle cells of ring layer in caudal part of rectum bound antibodies against cytokeratin 17 in addition to those against cytokeratins 8 and 18. Corresponding mesenchymal and smooth-muscle cells of adult individuals did not react with either of them. Cytokeratins were still synthesized when mesenchymal cells of embryonic intestine wall were cultivated in vitro. Intermediate filaments of these cells contain cytokeratins 8 and 18, as demonstrated by electron immunocytochemistry and immunoelectroblotting. Thus, the expression of cytokeratins is not restricted to adult and embryonic epithelial tissues but is also characteristic of mesenchyme and smooth muscle differentiation in human embryos and fetuses.

[Marker proteins of the differentiation of cultured human prostatic epithelial cells]. / Markernye belki differentsirovki kul'tiviruemykh épitelial'nykh kletok predstatel'noi zhelezy cheloveka.

The typical differences in the protein spectrum of cultured epithelial cells of the human definitive prostate and its premordium were revealed by the electrophoresis and electrofocusing procedure. Marker proteins typical of the cultured prostate epithelial cells, which underwent differentiation, have molecular weight of 70000-74000 Dalton and their isoelectric points are 6.6; 7; 7.5.

[In vitro models for research on cell differentiation and malignant degeneration in the human prostate]. / Modeli in vitro dlia izucheniia differentisirovki i malignizatsii kletok predstatel'noi zhelezy cheloveka.

The methods are reviewed for obtaining monolayer epithelium cultures both in normal and hyperplastic or malignant prostate glands. Preparation of pure epithelial tissue cultures is dealt with in detail. The major prostate cell lines are described. Special attention is paid to the markers of differentiation and sensitivity to hormones in normal and tumour prostatic cells in vitro. Prospects for the use of prostatic cell cultures as models in oncology are outlined.

[Substrate basis of the adhesion of the adenohypophyseal and diencephalic cell layers and cell anlagen in chick embryos]. / Substratnaia osnova adgezii kletochnykh plastov i kletok zakladok adenogipofiza i dientsefalona u zarodyshei kur

At the 16-18th stages of development of the chicken embryos adhesion of cell layers of the adenohypophysis and diencephalon anlages is provided with a submicroscopically regulated complex of neutral and acid polysaccharides, bivalent kations and probably proteins. The mechanisms of cells contact in each of the layers with this complex are different.

[Mechanisms of adenohypophyseal development in birds]. / K voprosu o mekhanizmakh razvitiia adenogipofiza u ptits

It was established by micrurgical methods that the second step of adenohypophysis morphogenesis related to the submergence of the Rathke pouch epithelium in the proliferating mesenchyme takes place due to high adhesivity of cells of the anterior wall of adenohypophysis anlage towards diencephalon base independently submerging in the mesenchyme. Cell adhesion ensures the direct contact of both the formations on the basis of which characteristic relationships between hypothalamus and adenohypophysis arise during development.