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1.
Sensors (Basel) ; 22(7)2022 Mar 24.
Artigo em Inglês | MEDLINE | ID: mdl-35408105

RESUMO

Rolling element bearing faults significantly contribute to overall machine failures, which demand different strategies for condition monitoring and failure detection. Recent advancements in machine learning even further expedite the quest to improve accuracy in fault detection for economic purposes by minimizing scheduled maintenance. Challenging tasks, such as the gathering of high quality data to explicitly train an algorithm, still persist and are limited in terms of the availability of historical data. In addition, failure data from measurements are typically valid only for the particular machinery components and their settings. In this study, 3D multi-body simulations of a roller bearing with different faults have been conducted to create a variety of synthetic training data for a deep learning convolutional neural network (CNN) and, hence, to address these challenges. The vibration data from the simulation are superimposed with noise collected from the measurement of a healthy bearing and are subsequently converted into a 2D image via wavelet transformation before being fed into the CNN for training. Measurements of damaged bearings are used to validate the algorithm's performance.


Assuntos
Aprendizado de Máquina , Redes Neurais de Computação , Algoritmos , Simulação por Computador , Vibração
2.
Nanomaterials (Basel) ; 9(12)2019 Dec 16.
Artigo em Inglês | MEDLINE | ID: mdl-31888231

RESUMO

Nanostructured surfaces and nanoparticles are already widely employed in many different fields of research, and there is an ever-growing demand for reliable, reproducible and scalable nanofabrication methods. This is especially valid for multifunctional nanomaterials with physical properties that are tailored for specific applications. Here, we report on the fabrication of two types of nanomaterials. Specifically, we present surfaces comprising a highly uniform array of elliptical pillars as well as nanoparticles with the shape of nanopockets, possessing nano-cavities. The structures are fabricated by nanoimprint lithography, physical and wet-chemical etching and sputter deposition of thin films of various materials to achieve a multifunctional nanomaterial with defined optical and magnetic properties. We show that the nanopockets can be transferred to solution, yielding a nanoparticle dispersion. All fabrication steps are carefully characterized by microscopic and optical methods. Additionally, we show optical simulation results that are in good agreement with the experimentally obtained data. Thus, this versatile method allows to fabricate nanomaterials with specific tailor-made physical properties that can be designed by modelling prior to the actual fabrication process. Finally, we discuss possible application areas of these nanomaterials, which range from biology and medicine to electronics, photovoltaics and photocatalysis.

3.
Opt Express ; 21(17): 20254-9, 2013 Aug 26.
Artigo em Inglês | MEDLINE | ID: mdl-24105570

RESUMO

We describe the fabrication of an antireflective surface structure with sub-wavelength dimensions on a glass surface using scalable low-cost techniques involving sol-gel coating, thermal annealing, and wet chemical etching. The glass surface structure consists of sand dune like protrusions with 250 nm periodicity and a maximum peak-to-valley height of 120 nm. The antireflective structure increases the transmission of the glass up to 0.9% at 700 nm, and the transmission remains enhanced over a wide spectral range and for a wide range of incident angles. Our measurements reveal a strong polarization dependence of the transmission change.

4.
Opt Lett ; 34(19): 3012-4, 2009 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-19794799

RESUMO

Microbolometers are modified by metallic resonant absorber elements, leading to an enhanced responsivity at selectable wavelengths. The dissipative energy absorption of tailored metamaterials allows for engineering the response of conventional bolometer microbridges. The absorption peak position and height are determined by the geometry of the metamaterial. Square-shaped metal/dielectric/metal stacks as absorber elements show spectral resonances at wavelengths between 4.8 and 7.0 microm in accordance with numerical simulations. Total peak absorptions of 0.8 are obtained. The metamaterial modified bolometers are suitable for multispectral thermal imaging systems in the mid-IR and terahertz regime.

5.
Anal Chem ; 81(20): 8503-12, 2009 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-19754102

RESUMO

An under recognized cause of preventable mortality is healthcare-associated (nosocomial) infections such as biofilms found on implants and catheters. About 5% of U.S. and E.U. patients acquire nosocomial infections leading to prolonged hospitalization, increased patient suffering, and mortality rates. To date, no satisfactory solutions are available to monitor biofilm formation under near-native conditions. As a consequence, in the present work, we report the development of a disposable microfluidic biochip capable of continuously monitoring cell population dynamics under physiological shear force conditions. We demonstrate the simultaneous application of contactless bioimpedance spectroscopy and amperometric measurements to monitor fungal biofilm growth rates and metabolic activities. Quantitative cell analysis is accomplished by the use of high-density interdigitated capacitors (microIDC) isolated by a 700 nm epoxy (SU-8 resist) based passivation layer to noninvasively assess biofilm formation in predefined proliferation chambers. Additionally, biofilm respiration activity is measured using redox-mediators oxidized at band electrodes located downstream within microchannels. The disposable biofilm analysis platform is used to continuously monitor the dynamic responses of C. albicans to different glucose and galactose concentrations.


Assuntos
Biofilmes , Candida albicans/citologia , Candida albicans/fisiologia , Equipamentos Descartáveis , Técnicas Analíticas Microfluídicas/métodos , Candida albicans/crescimento & desenvolvimento , Candida albicans/isolamento & purificação , Permeabilidade da Membrana Celular , Contagem de Colônia Microbiana , Simulação por Computador , Impedância Elétrica , Eletroquímica , Transporte de Elétrons , Técnicas Analíticas Microfluídicas/instrumentação , Modelos Biológicos , Propriedades de Superfície
6.
Lab Chip ; 7(12): 1723-31, 2007 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-18030393

RESUMO

Microfabricated biochips are developed to continuously monitor cell population dynamics in a non-invasive manner. In the presented work we describe the novel combination of contact-less dielectric microsensors and microfluidics to promote biofilm formation for quantitative cell analysis. The cell chip consists of a polymeric fluidic (PDMS) system bonded to a glass wafer containing the electrodes while temperature and fluid flow are controlled by external heating and pumping stations. The high-density interdigitated capacitors (microIDES) are isolated by a 550 nm multi-passivation layer of defined dielectric property and provide stable, robust and non-drifting measurement conditions. The performance of this detector is evaluated using various bacterial and yeast strains. The high sensitivity of the developed dielectric microsensors allows direct identification of microbial strains based on morphological differences and biological composition. The novel biofilm analysis platform is used to continuously monitor the dynamic responses of C. albicans and P. pastoris biofilms to increased shear stress and antimicrobial agent concentration. While the presence of shear stress triggers significant changes in yeast growth profiles, the addition of 0.5 microg mL(-1) amphotericin B revealed two distinct dynamic behaviors of the C. albicans biofilm. Initially, impedance spectra increased linearly at 30 Omega h(-1) for two hours followed by 10 Omega h(-1) (at 50 kHz) over 10 hours while cell viability remained above 95% during fungicide administration. These results demonstrate the ability to directly monitor dielectric changes of sub-cellular components within a living cell population.


Assuntos
Biofilmes , Técnicas Analíticas Microfluídicas/instrumentação , Técnicas Analíticas Microfluídicas/métodos , Sistemas On-Line/instrumentação , Membrana Celular
7.
Biosens Bioelectron ; 20(8): 1685-9, 2005 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-15626629

RESUMO

The bondforces between biotinylated surfaces and streptavidin or avidin coated beads are investigated by a magnetic field based manipulation system for magnetic microbeads. The magnetic field is generated by currents through a set of conducting lines, and its gradient exerts a force onto the magnetic beads. The force can be increased until the bond between the bead and the surface breaks. Consistent with other groups we found two conformations for both investigated bonds. The measured bondforces for the two conformations are for Streptavidin-Biotin: 55.9 and 244.7 fN and for Avidin-Biotin: 15.9 and 58.4 fN. These very low bondforces (10-100 times smaller than earlier measurements) match to the extremely low loading rate of about 1 fN/s. This new technique thus allows to investigate biomolecular bonds by extremely low forces.


Assuntos
Avidina/química , Biotina/química , Magnetismo , Micromanipulação/métodos , Estreptavidina/química , Avidina/análise , Avidina/ultraestrutura , Sítios de Ligação , Biotina/análise , Micromanipulação/instrumentação , Microesferas , Estimulação Física/instrumentação , Estimulação Física/métodos , Ligação Proteica , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Estreptavidina/análise , Estreptavidina/ultraestrutura , Estresse Mecânico
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