RESUMO
The human embryonic stem cell line RCe021-A (RC-17) was derived under quality assured compliance with UK regulation, European Union Directives and International guidance for tissue procurement, processing and storage according to Good Manufacturing Practice (GMP) standards. The cell line was derived from a day 3 embryo voluntarily donated as unsuitable or surplus to fertility requirements following informed consent. RCe021-A (RC-17) shows normal pluripotency marker expression and differentiation to the three germ layers in vitro. It has a normal 46XX female karyotype and microsatellite PCR identity, HLA and blood group typing data are available.
RESUMO
The human embryonic stem cell line RCe013-A (RC-9) was derived under quality assured compliance with UK regulation, European Union Directives and International guidance for tissue procurement, processing and storage according to Good Manufacturing Practice (GMP) standards. The cell line was derived from a failed to fertilise oocyte voluntarily donated as unsuitable and surplus to fertility requirements following informed consent. RCe013-A (RC-9) shows normal pluripotency marker expression and differentiation to the three germ layers in vitro and in vivo. It has a normal 46XY male karyotype and microsatellite PCR identity, HLA and blood group typing data are available.
RESUMO
The human embryonic stem cell line RCe015-A (RC-11) was derived under quality assured compliance with UK regulation, European Union Directives and International guidance for tissue procurement, processing and storage according to Good Manufacturing Practice (GMP) standards. The cell line was derived from a fragmented cleavage stage embryo voluntarily donated as unsuitable or surplus to fertility requirements following informed consent. RCe015-A (RC-11) shows normal pluripotency marker expression and differentiation to the three germ layers in vitro and in vivo. It has a normal 46XX female karyotype and microsatellite PCR identity, HLA and blood group typing data are available.
RESUMO
The human embryonic stem cell line RCM-1 was derived from a failed to fertilise egg undergoing parthenogenetic stimulation. The cell line shows normal pluripotency marker expression and differentiation to three germ layers in vitro and in vivo. It has a normal 46XX female karyotype and microsatellite PCR identity, HLA and blood group typing data is available.
Assuntos
Células-Tronco Embrionárias Humanas/citologia , Óvulo/citologia , Animais , Diferenciação Celular , Células Cultivadas , Reprogramação Celular , Hibridização Genômica Comparativa , Corpos Embrioides/citologia , Feminino , Citometria de Fluxo , Teste de Histocompatibilidade , Células-Tronco Embrionárias Humanas/metabolismo , Células-Tronco Embrionárias Humanas/transplante , Humanos , Cariótipo , Camundongos , Camundongos SCID , Repetições de Microssatélites/genética , Microscopia de Fluorescência , Teratoma/metabolismo , Teratoma/patologia , Fatores de Transcrição/genética , Fatores de Transcrição/metabolismo , Transplante HeterólogoRESUMO
The human embryonic stem cell line RCe010-A (RC-6) was derived from a frozen and thawed blastocyst voluntarily donated as unsuitable and surplus to fertility requirements following ethics committee approved informed consent under licence from the UK Human Fertilisation and Embryology Authority. The cell line shows normal pluripotency marker expression and differentiation to the three germ layers in vitro. It has a normal 46XY male karyotype and microsatellite PCR identity, HLA and blood group typing data are available.
Assuntos
Blastocisto/citologia , Células-Tronco Embrionárias Humanas/citologia , Alelos , Diferenciação Celular , Células Cultivadas , Reprogramação Celular , Corpos Embrioides/citologia , Genótipo , Teste de Histocompatibilidade , Células-Tronco Embrionárias Humanas/metabolismo , Humanos , Cariótipo , Antígenos CD15/metabolismo , Masculino , Repetições de Microssatélites/genética , Microscopia de Fluorescência , Fatores de Transcrição/genética , Fatores de Transcrição/metabolismoRESUMO
The human embryonic stem cell line RCe011-A (RC-7) was derived from a failed to fertilise oocyte voluntarily donated as unsuitable and surplus to fertility requirements following ethics committee approved informed consent under licence from the UK Human Fertilisation and Embryology Authority. The cell line shows normal pluripotency marker expression and differentiation to the three germ layers in vitro. It has a normal 46XY male karyotype and microsatellite PCR identity, HLA and blood group typing data are available.
Assuntos
Embrião de Mamíferos/citologia , Células-Tronco Embrionárias Humanas/citologia , Alelos , Diferenciação Celular , Células Cultivadas , Corpos Embrioides/citologia , Genótipo , Teste de Histocompatibilidade , Células-Tronco Embrionárias Humanas/metabolismo , Humanos , Cariótipo , Antígenos CD15/metabolismo , Masculino , Repetições de Microssatélites/genética , Microscopia de Fluorescência , Fator 3 de Transcrição de Octâmero/metabolismo , Reação em Cadeia da PolimeraseRESUMO
The human embryonic stem cell line RCe012-A (RC-8) was derived from a frozen and thawed day 5 embryo cultivated to the blastocyst stage. The embryo was voluntarily donated as unsuitable and surplus to fertility requirements following ethics committee approved informed consent under licence from the UK Human Fertilisation and Embryology Authority. The cell line shows normal pluripotency marker expression and differentiation to the three germ layers in vitro. It has a normal 46XX female karyotype and microsatellite PCR identity, HLA and blood group typing data is available.
Assuntos
Blastocisto/citologia , Células-Tronco Embrionárias Humanas/citologia , Alelos , Diferenciação Celular , Células Cultivadas , Corpos Embrioides/citologia , Feminino , Genótipo , Teste de Histocompatibilidade , Células-Tronco Embrionárias Humanas/metabolismo , Humanos , Cariótipo , Antígenos CD15/metabolismo , Repetições de Microssatélites/genética , Microscopia de Fluorescência , Fator 3 de Transcrição de Octâmero/metabolismo , Reação em Cadeia da PolimeraseRESUMO
The human embryonic stem cell line RCe014-A (RC-10) was derived from a fresh oocyte voluntarily donated as unsuitable and surplus to fertility requirements following ethics committee approved informed consent under licence from the UK Human Fertilisation and Embryology Authority. The cell line shows normal pluripotency marker expression and differentiation to the three germ layers in vitro. It has a mixed 46XY and 47XY +12 male karyotype and microsatellite PCR identity, HLA and blood group typing data is available.
Assuntos
Células-Tronco Embrionárias Humanas/citologia , Diferenciação Celular , Células Cultivadas , Cromossomos Humanos Par 12 , Embrião de Mamíferos/citologia , Corpos Embrioides/citologia , Genótipo , Teste de Histocompatibilidade , Células-Tronco Embrionárias Humanas/metabolismo , Humanos , Imuno-Histoquímica , Cariótipo , Masculino , Repetições de Microssatélites/genética , Microscopia de Fluorescência , Oócitos/citologia , Fatores de Transcrição/metabolismo , TrissomiaRESUMO
The human embryonic stem cell line RCe019-A (RC-15) was derived under quality assured compliance with UK regulation, European Union Directives and International guidance for tissue procurement, processing and storage according to Good Manufacturing Practice (GMP) standards. The cell line was derived from a cleavage stage embryo voluntarily donated as unsuitable or surplus to fertility requirements following informed consent. RCe019-A (RC-15) shows normal pluripotency marker expression and differentiation to the three germ layers in vitro. It has a mixed 46XX/47XX, +8 female karyotype and microsatellite PCR identity, HLA and blood group typing data is available.
Assuntos
Blastocisto/citologia , Células-Tronco Embrionárias Humanas/citologia , Antígenos de Grupos Sanguíneos/metabolismo , Diferenciação Celular , Células Cultivadas , Reprogramação Celular , Feminino , Genótipo , Teste de Histocompatibilidade , Células-Tronco Embrionárias Humanas/metabolismo , Humanos , Cariótipo , Repetições de Microssatélites/genética , Fatores de Transcrição/genética , Fatores de Transcrição/metabolismoRESUMO
The human embryonic stem cell line RCe017-A (RC-13) was derived under quality assured compliance with UK regulation, European Union Directives and International guidance for tissue procurement, processing and storage according to Good Manufacturing Practice (GMP) standards. The cell line was derived from a frozen and thawed blastocyst stage embryo voluntarily donated as unsuitable or surplus to fertility requirements following informed consent. RCe017-A (RC-13) shows normal pluripotency marker expression and differentiation to the three germ layers in vitro. It has a mixed 47XY, +12/48XY, +1, +12 male karyotype and microsatellite PCR identity, HLA and blood group typing data are available.
Assuntos
Blastocisto/citologia , Células-Tronco Embrionárias Humanas/citologia , Diferenciação Celular , Células Cultivadas , Reprogramação Celular , Cromossomos Humanos Par 12 , Genótipo , Teste de Histocompatibilidade , Células-Tronco Embrionárias Humanas/metabolismo , Humanos , Cariótipo , Masculino , Repetições de Microssatélites/genética , Fatores de Transcrição/genética , Fatores de Transcrição/metabolismo , TrissomiaRESUMO
The human embryonic stem cell line RCe018-A (RC-14) was derived under quality assured compliance with UK regulation, European Union Directives and International guidance for tissue procurement, processing and storage according to Good Manufacturing Practice (GMP) standards. The cell line was derived from a blastocyst stage embryo voluntarily donated as unsuitable or surplus to fertility requirements following informed consent. RCe018-A (RC-14) shows normal pluripotency marker expression and differentiation to the three germ layers in vitro. It has a male karyotype with an extra copy of chromosome 8 (47XY, +8). Microsatellite PCR identity, HLA and blood group typing data are available.
Assuntos
Blastocisto/citologia , Células-Tronco Embrionárias Humanas/citologia , Antígenos de Grupos Sanguíneos/metabolismo , Diferenciação Celular , Células Cultivadas , Reprogramação Celular , Cromossomos Humanos Par 8 , Genótipo , Teste de Histocompatibilidade , Células-Tronco Embrionárias Humanas/metabolismo , Humanos , Cariótipo , Masculino , Repetições de Microssatélites/genética , Fatores de Transcrição/genética , Fatores de Transcrição/metabolismo , TrissomiaRESUMO
The human embryonic stem cell line RCe016-A (RC-12) was derived under quality assured compliance with UK regulations, EU Directives and International guidance for tissue procurement, processing and storage according to good manufacturing practice (GMP) standards. The cell line was derived from a cryopreserved blastocyst stage embryo voluntarily donated as surplus to fertility requirements following informed consent. RCe016-A (RC-12) shows normal pluripotency marker expression and differentiation to three germ layers in vitro. Karyology revealed a mixed male karyotype at early passage (P15), which resolved as normal 46XY by passage 33. Microsatellite PCR identity, HLA and blood group typing data is available.
Assuntos
Blastocisto/citologia , Células-Tronco Embrionárias Humanas/citologia , Antígenos de Grupos Sanguíneos/metabolismo , Diferenciação Celular , Células Cultivadas , Reprogramação Celular , Genótipo , Teste de Histocompatibilidade , Células-Tronco Embrionárias Humanas/metabolismo , Humanos , Cariótipo , Masculino , Repetições de Microssatélites/genética , Fatores de Transcrição/genética , Fatores de Transcrição/metabolismoRESUMO
The human embryonic stem cell line RCe020-A (RC-16) was derived under quality assured compliance with UK regulation, European Union Directives and International guidance for tissue procurement, processing and storage according to Good Manufacturing Practice (GMP) standards. The cell line was derived from a failed to fertilise oocyte voluntarily donated as unsuitable or surplus to fertility requirements following informed consent. RCe020-A (RC-16) shows normal pluripotency marker expression and differentiates to mesoderm and potentially ectoderm in vitro. It has an abnormal 47XX, +14, i(20)(q10) female karyotype and microsatellite PCR identity, HLA and blood group typing data is available.
Assuntos
Blastocisto/citologia , Células-Tronco Embrionárias Humanas/citologia , Antígenos de Grupos Sanguíneos/metabolismo , Diferenciação Celular , Células Cultivadas , Reprogramação Celular , Feminino , Genótipo , Teste de Histocompatibilidade , Células-Tronco Embrionárias Humanas/metabolismo , Humanos , Cariótipo , Repetições de Microssatélites/genética , Fatores de Transcrição/genética , Fatores de Transcrição/metabolismoRESUMO
This was a retrospective study reviewing 68 hip arthroplasties performed with noncemented, porous-coated components at three institutions in the period between December 1983 and June 1987. Fifty of these were available for follow-up study. The mean follow-up period was three years (range, 18-56 months). Thirty-seven patients were treated by total hip arthroplasty (THA) with porous-coated components, and thirteen were implanted with porous-coated femoral stems with bipolar endoprostheses using a porous-coated femoral stem and a universal head. The mean Harris hip score at follow-up evaluation for the entire group was 85.7 points. Eighty percent of the patients had either a good or excellent result. Those patients implanted with a porous femoral stem with bipolar acetabular components had only 54% good or excellent results compared with 90% good or excellent results for those having THA. Roentgenographic findings included a 59% rate of femoral neck cortex osteopenia, an 80% rate of medial or lateral distal cortical hypertrophy, and an 80% rate of distal bone bridging. All of these roentgenographic changes were secondary to changes in stress transference to the proximal femur. There was no correlation between thigh pain or the degree of bone-prosthesis radiolucencies and the fit of the femoral stem.
