RESUMO
Larval fishes provide a valuable metric for assessing and monitoring species, populations, and ecosystem trends and condition. However, taxonomic resolution for this life stage is inherently problematic because of their individual sizes, limited morphological characteristics and high tissue degradation rates. There is little research on methods that rapidly preserve larval tissues for later morphological and molecular identification. The goal of this study was to test methods of rapidly killing fish embryos that maintain both morphological and molecular integrity. Rapid cooling with dry ice successfully maintained morphological and molecular integrity and may offer a simple and cost-effective approach for larval fish identification.
Assuntos
Gelo-Seco , Peixes/anatomia & histologia , Peixes/classificação , Preservação Biológica/métodos , Animais , Código de Barras de DNA Taxonômico , Embrião não Mamífero/anatomia & histologiaRESUMO
Music can influence clinicians' and patients' mental states and emotions via the capacity of rhythm and tone to entrain. Entrainment can facilitate relaxation and distraction from pain and has a role to play in experiences with and in health care. In this article, we discuss the benefits of music from the perspectives of a physician and a veteran.
