Acupoint injection of autologous stromal vascular fraction and allogeneic adipose-derived stem cells to treat hip dysplasia in dogs.

Stem cells isolated from adipose tissue show great therapeutic potential in veterinary medicine, but some points such as the use of fresh or cultured cells and route of administration need better knowledge. This study aimed to evaluate the effect of autologous stromal vascular fraction (SVF, n = 4) or allogeneic cultured adipose-derived stem cells (ASCs, n = 5) injected into acupuncture points in dogs with hip dysplasia and weak response to drug therapy. Canine ASCs have proliferation and differentiation potential similar to ASCs from other species. After the first week of treatment, clinical evaluation showed marked improvement compared with baseline results in all patients treated with autologous SVF and three of the dogs treated with allogeneic ASCs. On days 15 and 30, all dogs showed improvement in range of motion, lameness at trot, and pain on manipulation of the joints, except for one ASC-treated patient. Positive results were more clearly seen in the SVF-treated group. These results show that autologous SVF or allogeneic ASCs can be safely used in acupoint injection for treating hip dysplasia in dogs and represent an important therapeutic alternative for this type of pathology. Further studies are necessary to assess a possible advantage of SVF cells in treating joint diseases.

Modelo experimental de melanoma em camundongos / Experimental model of murine melanoma in mice

O melanoma cutâneo é neoplasia maligna que tem origem na pele, correspondendo a cerca de 1 a 3 porcento de todos os tumores malignos. Ocorre em igual proporçäo em homens e mulheres e acomete principalmente pessoas de pele clara, com pico de incidência entre quarenta e sessenta anos. As modalidades tradicionais de tratamento de melanoma metastático vêm apresentando resultados insatisfatórios. Logo, os estudos in vitro e in vivo säo de fundamental importâcia para o melhor entendimento do comportamento biológico dessa neoplasia e para o desenvolvimento diagnóstico e terapêutico. A linhagem celular de melanoma murino B16-F10 e sua inoculaçäo por via intraperitoneal ou subcutânea em camundongos C57BL/6N säo modelos experimentais vantajosos para testar grande número de de drogas e selecionar compostos específicos para esse tumor cutâneo. Säo descritos o manejo da cepa C57BL/6N em biotério e suas principais características, bem como a forma de apresentaçäo clínica do melanoma murino B16-F10 e suas possibilidades em termos experimentais