RESUMO
Eight short tandem repeat markers included in the International Society for Animal Genetics panel of 24 loci investigated in canine comparison tests were analysed in a sample of pure-breed dogs, with the purpose of defining an allele nomenclature based on the number of repeats. A regression analysis of the raw data produced by the sequencer, coupled with the direct sequencing of selected alleles, allowed us to propose a system of nomenclature for six of the eight loci (four di-nucleotidic: AHT121, AHTh137, REN169018 and REN64E19, and two tetra-nucleotidic: FH2001 and FH2328). The remaining two loci (INU055 and FH2848) showed a pattern of fragments that did not resolve in a simple allele series. This work may be useful to establish a basis for comparing data across different laboratories for a set of validated canine markers, which can be used in population genetics, forensics and other analyses.
Assuntos
Cães/classificação , Cães/genética , Marcadores Genéticos , Repetições de Microssatélites , Alelos , Animais , Cruzamento , Frequência do Gene , Loci Gênicos , Padrões de Referência , Análise de Sequência de DNARESUMO
Se evaluó con la técnica de Bramante, Berbert, Borges, la preparación del tercio cervical de los conductos mesial y distal de molares superiores con fresa Gates Glidden, e instrumentos rotatorios de Nifi, Orifece Shaper y ProTaper. Treinta dientes molares superiores fueron elegidos. Después de hecho el acceso a la cámara pulpar, se preparo el tercio cervical con la fresa de Gates Glidden e instrumentos de Nifi, Orifece Shaper y ProTaper. Se analizó el conducto y el ProTaper fue el que menos desgastó (AU)
The aims of this study was to analyce cervical not preparation with Gates Glidden bur, Orifice Shaper and ProTaper NiTi rotary instruments. Thirty maxillary molars were prepared with this instruments and analised with the Bramante Berter, Borges technique. Dont have difference between Gates Glidden but, Orifice Shaper and ProTaper instruments in preparation of the cervical third of root canal But, ProTaper was better maintenance root canal anatomy (AU)
