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1.
RBM rev. bras. med ; 69(supl.9)nov. 2012.
Artigo em Português | LILACS | ID: lil-740534
2.
Magn Reson Imaging ; 21(10): 1151-7, 2003 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-14725922

RESUMO

To study the sensitivity of intermolecular double quantum coherences (iDQc) imaging contrast to brain microstructure and brain anisotropy, we investigated the iDQC contrast between differently structured areas of the brain according to the strength and the direction of the applied correlation gradient. Thus diffusion-weighted imaging (DWI) and diffusion tensor imaging (DTI) maps have been obtained. This procedure, which consists of analyzing both iDQc and DWI images at different gradient strength and gradient direction, could be a promising tool for clinical brain investigations performed with higher than 1.5 T magnetic fields.


Assuntos
Encéfalo/anatomia & histologia , Imagem de Difusão por Ressonância Magnética , Anisotropia , Humanos
3.
Arch Toxicol ; 69(3): 165-70, 1995.
Artigo em Inglês | MEDLINE | ID: mdl-7717872

RESUMO

A comparative biochemical and histological study on the hepatotoxicity of a single dose of N,N-dimethylformamide (DMF) and N-methylformamide (NMF) in control and acetone-treated SD male rats and CD-1 male mice was performed. In control and acetone-pretreated rats, neither DMF nor NMF caused hepatic damage or elevation of plasma transaminases. In contrast, in acetonized but not in control mice, DMF administration yielded some evidence of liver necrosis and elevation of ALAT (alanine-amino transferase) activity. After a DMF dose of 1000 mg/kg, ALAT activity was found 1215 +/- 832 mU/ml and 47 +/- 18 mU/ml in acetonized and control mice, respectively. NMF treatment was hepatotoxic in control mice and lethal in acetonized mice. In control mice, an NMF dose of 600 mg/kg increased ALAT activity from a basal value of 35 +/- 5 to 2210 +/- 1898 mU/ml. When the oxidative metabolism of DMF was investigated, microsomes from both rats and mice preinduced by acetone increased the demethylation rate of DMF 7 to 10-fold compared to that (about 0.25 nmol/min per mg protein) of the corresponding control microsomes. The enzymatic affinities for DMF oxidation, however, were different: in mice the Km (0.05 mM) was one order of magnitude lower than that (0.56 mM) found in rats. The experiments performed with purified rat and mouse P-450 2E1 in a reconstituted system confirmed that the P-450 2E1 isoforms are very active catalysts towards DMF oxidation (the turnover was about 10 nmol/min per nmol P-450 for both enzymes) but with a strikingly different affinity. Whereas the Km for mouse P-450 2E1 was 0.08 +/- 0.03 mM, that for rat P-450 2E1 was 1.1 +/- 0.2 mM.(ABSTRACT TRUNCATED AT 250 WORDS)


Assuntos
Sistema Enzimático do Citocromo P-450/metabolismo , Dimetilformamida/metabolismo , Dimetilformamida/toxicidade , Fígado/efeitos dos fármacos , Oxirredutases N-Desmetilantes/metabolismo , Animais , Citocromo P-450 CYP2E1 , Formamidas/metabolismo , Formamidas/toxicidade , Fígado/enzimologia , Fígado/patologia , Masculino , Camundongos , Camundongos Endogâmicos , Oxirredução , Ratos , Ratos Sprague-Dawley
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