Adolescent ethanol exposure and differential rearing environment affect taste reactivity to ethanol in rats.

The identification and characterization of variables that influence "liking" and enhance vulnerability to repeated alcohol use are vital to understanding and treating alcohol use disorders. In the current study, we explore the influence of rearing environment and experimenter-administered adolescent ethanol on the hedonic value of ethanol, sucrose, and quinine. Male and female rats were reared for 30 days starting at postnatal day (PND) 21 in either an enriched, isolated, or standard condition and received 1.5 g/kg (intraperitoneally [i.p.]) 20% (w/v) ethanol or saline every other day for 12 days starting at PND 28. Thereafter, all rats had indwelling intraoral fistulae implanted and their taste reactivities to water, ethanol (5, 10, 20, 30, 40% v/v), sucrose (0.1, 0.25, 0.5 M), and quinine (0.1, 0.5 mM) were recorded and analyzed. Results indicated that enrichment elevated hedonic responding to sucrose compared to isolation, and induced a stronger negative relationship between hedonic responding and ethanol concentration compared to standard conditions. Enrichment also elevated aversive responding to ethanol and quinine compared to both isolated and standard condition rats. Adolescent ethanol injections marginally reduced aversive responding to quinine. These results replicate previous findings that environmental enrichment enhances both "liking" and aversion. In addition, the current findings suggest that, while adolescent ethanol injections may blunt aversive responses to quinine, they have no effect on aversive or hedonic responding to ethanol or sucrose. Together with existing literature, our results may suggest that experience with the taste of ethanol is necessary for alterations to ethanol "liking" and aversion.

Differential rearing alters taste reactivity to ethanol, sucrose, and quinine.

RATIONALE: Early-life environment influences reinforcer and drug motivation in adulthood; however, the impact on specific components of motivation, including hedonic value ("liking"), remains unknown. OBJECTIVES: The current study determined whether differential rearing alters liking and aversive responding to ethanol, sucrose, and quinine in an ethanol-naïve rat model. METHODS: Male and female rats were reared for 30 days starting at postnatal day 21 in either an enriched (EC), isolated (IC), or standard condition (SC). Thereafter, all rats had indwelling intraoral fistulae implanted and their taste reactivity to water, ethanol (5, 10, 20, 30, 40% v/v), sucrose (0.1, 0.25, 0.5 M), and quinine (0.1, 0.5 mM) was recorded and analyzed. RESULTS: EC rats had higher amounts of liking responses to ethanol, sucrose, and quinine and higher amounts of aversive responses to ethanol and quinine compared to IC rats. While EC and IC rats' responses were different from each other, they both tended to be similar to SCs, who fell in between the EC and IC groups. CONCLUSIONS: These results suggest that environmental enrichment may enhance sensitivity to a variety of tastants, thereby enhancing liking, while isolation may dull sensitivity, thereby dulling liking. Altogether, the evidence suggests that isolated rats have a shift in the allostatic set-point which may, in part, drive increased responding for a variety of rewards including ethanol and sucrose. Enriched rats have enhanced liking of both sucrose and ethanol suggesting that enrichment may offer a unique phenotype with divergent preferences for incentive motivation.