Bioaccumulation potential of the tricyclic antidepressant amitriptyline in a marine Polychaete, Nereis virens.

The continual discharge of pharmaceuticals from wastewater treatment plants (WWTPs) into the marine environment, even at concentrations as low as ng/L, can exceed levels that induce sublethal effects to aquatic organisms. Amitriptyline, a tricyclic antidepressant, is the most prescribed antidepressant in Norway, though the presence, potential for transport, and uptake by aquatic biota have not been assessed. To better understand the release and bioaccumulative capacity of amitriptyline, laboratory exposure studies were carried out with field-collected sediments. Influent and effluent composite samples from the WWTP of Stavanger (the 4th largest city in Norway) were taken, and sediment samples were collected in three sites in the proximity of this WWTP discharge at sea (WWTP discharge (IVAR), Boknafjord, and Kvitsøy (reference)). Polychaetes (Nereis virens) were exposed to field-collected sediments, as well as to Kvitsøy sediment spiked with 3 and 30 µg/g amitriptyline for 28 days. The WWTP influent and effluent samples had concentrations of amitriptyline of 4.93 ± 1.40 and 6.24 ± 1.39 ng/L, respectively. Sediment samples collected from IVAR, Boknafjord, and Kvitsøy had concentrations of 6.5 ± 3.9, 15.6 ± 12.7, and 12.7 ± 8.0 ng/g, respectively. Concentrations of amitriptyline were below the limit of detection in polychaetes exposed to sediment collected from Kvitsøy and IVAR, and 5.2 ± 2.8 ng/g in those exposed to Boknafjord sediment. Sediment spiked with 3 and 30 µg/g amitriptyline had measured values of 423.83 ± 33.1 and 763.2 ± 180.5 ng/g, respectively. Concentrations in worms exposed to the amended sediments were 9.5 ± 0.2 and 56.6 ± 2.2 ng/g, respectively. This is the first known study to detect measurable concentrations of amitriptyline in WWTP discharge in Norway and accumulation in polychaetes treated with field-collected sediments, suggesting that amitriptyline has the potential for trophic transfer in marine systems.

Effects of oil pollution and persistent organic pollutants (POPs) on glycerophospholipids in liver and brain of male Atlantic cod (Gadus morhua).

Fish in the North Sea are exposed to relatively high levels of halogenated compounds in addition to the pollutants released by oil production activities. In this study male Atlantic cod (Gadus morhua) were orally exposed to environmental realistic levels (low and high) of weathered crude oil and/or a mixture of POPs for 4weeks. Lipid composition in brain and in liver extracts were analysed in order to assess the effects of the various pollutants on membrane lipid composition and fatty acid profiles. Transcriptional effects in the liver were studied by microarray and quantitative real-time RT-PCR. Chemical analyses confirmed uptake of polychlorinated biphenyls (PCBs) and chlorinated pesticides, polybrominated diphenyl ethers (PBDEs) and perfluorooctanesulfonate (PFOS) in the liver and excretion of metabolites of polyaromatic hydrocarbons (PAHs) in the bile. Treatment with POPs and/or crude oil did not induce significant changes in lipid composition in cod liver. Only a few minor changes were observed in the fatty acid profile of the brain and the lipid classes in the liver. The hypothesis that pollution from oil or POPs at environmental realistic levels alters the lipid composition in marine fish was therefore not confirmed in this study. However, the transcriptional data suggest that the fish were affected by the treatment at the mRNA level. This study suggests that a combination of oil and POPs induce the CYP1a detoxification system and gives an increase in the metabolism and clearing rate of PAHs and POPs, but with no effects on membrane lipids in male Atlantic cod.