[Relation between milk production and biochemical indicators in high- and low-producing cows]. / Vztahy mliekovej úzitkovosti a biochemických ukazovatel'ov u vysoko a menej úzitkových kráv.

23 cows of the Holstein-Friesian breed during second lactation were used in the experiment. All animals were housed in one stable with tying. The blood was collected from each cow four times during first to third, fourth to sixth, seventh to ninth and above nine months of lactation. After 305 days of lactation, the milk cows were classified as with high (n = 14) and lower performance (n = 9), the limit value was 5000 kg of milk. The difference in milk production between groups was highly significant even during the period of the first 90 days (501 kg) and this difference increased for 305 days of lactation to 1538 kg (Tab. I); the dairy cows with high performance produced 6162 kg of milk per 305 days and that with the lower performance gave 4624 kg during the same period. Another significant differences occurred in the fat contents per kg and in the amount of milk converted on 4% fat content. During the first stage of lactation a slightly higher haemoglobine level was found in cows with high performance, the opposing results were obtained in another studies (Tab. II), the highest difference was recorded in the course of 7th to 9th month. The level of total protein was, except 7th to 9th month, always higher in cows of the first group. The difference was significant at the beginning of lactation. Insulin concentration (INS) exhibited identical tendency for the whole lactation--dairy cows with high milk production exhibited lower values, significant differences were recorded in the first observation at the beginning of lactation and in the period above 9 months. Similarly as in case of insulin, it is also in hormones of thyroid gland, the cows with high milk capacity had the lower values (Tab. III). The highest differences in triiodothyronine (T3) concentration were recorded in the second half of lactation, significance ranged from 7th to 9th month of lactation. In thyroxine content (T4), marked differences were recorded during the first and last observations, the difference was highly statistically significant during the seventh to ninth month of lactation. The levels of cyclic adenosine monophosphate (AMP) were higher for the whole experiment in animals with lower milk production. Tab. IV gives the correlation coefficient of different parameters between periods under study. Almost all parameters are in a close positive relation, the closest dependences were observed between second, third and fourth periods in hormones of thyreoidea. The most significances were recorded in adenosine monophosphate, thus confirming its stability of minimum variance between observations.(ABSTRACT TRUNCATED AT 400 WORDS)

[The health status and metabolic indicators in calves fed colostrum and first milk preserved with potassium sorbate and milk replacements]. / Zdravotný stav a metabolické ukazovatele u teliat napájaných mledzivom a nezrelým mliekom konzervovaným sorbanom draselným a mliecnou krmnou zmesou.

The aim of the experiments was to investigate the effects of the preservation of colostrum and unripe milk with potassium sorbate at a concentration of 0.5% and of its storage for three to five days at normal environmental temperatures on the calf health condition. A set of 30 calves divided into two groups was included in the trial. Calves belonging to the experimental group were fed preserved colostrum and unripe milk from day 9 to day 30 of their life (the first experimental period), and from day 31 to day 50 (the second experimental period) milk replacer. Calves belonging to the control group were fed milk replacer from day 9 to day 50 of their life. One calf from the experimental group died from a respiratory disease. In the control group, one calf died and another had to be slaughtered; in both cases digestive tract diseases were diagnosed. The number of calves which had to be treated medically was also lower in the experimental group--in the calves fed preserved colostrum--one case of periodic tympany was recorded in comparison with four cases in the control group. When haematological and biochemical characteristics were examined, significantly lower haemoglobin levels were found in experimental calves at the end of the first experimental period (105.4 g.l-1 when compared with 112 g.l-1 in the control group) and significantly higher values of proteinaemia were found in the blood plasma of these calves at the same period (59.6 g.l-1 and 53.8 g.l-1, respectively). Similar differences were also found during a single haematological and biochemical examination.

[The effect of artificial ultraviolet light on biochemical indicators in calves]. / Vplyv umelého ultrafialového svetla na biochemické ukazovatele teliat.

Changes in the biochemical parameters of the blood serum of calves were studied after exposure to optimum doses of ultraviolet radiation. The calves were exposed to UV-light twice daily in three periods: 8, 9 and 10 days. Daily doses of 90, 130 and 180 mEr.h.m-2 were used in the first experiment, 120, 180 and 240 mEr.h.m-2 in the second experiment. As found, exposure to radiation in the individual periods increased total proteinaemia, the differences between the experimental and control groups being significant. Exposure to ultraviolet light induced an increase in the activity of the thyroid gland; at the end of the periods of UV-radiation the concentration of thyroxine in the experimental groups was significantly or highly significantly higher. The largest differences were recorded in the first trial (15 nmol per litre to 21.4 nmol per litre). No changes were detected by the evaluation of the activities of alkaline phosphatase, triiodothyronine, sodium, potassium and calcium and in the sodium: potassium ratio.

[Changes in biochemical indicators in dairy cows induced by extremely low temperatures]. / Zmeny biochemických ukazovatel'ov dojníc vyvolané extrémne nízkymi teplotami.

The objective of the experiment was to find out the effect of extremely low temperatures on the biochemical characteristics of the cows. Twenty-one dairy cows were used as the experimental material. The experimental group (n = 12) was kept loose in an open barn without thermal insulation and the control group (n = 9) was kept in an insulated cow-house. The winter season included two periods of extremely low temperatures: from the 3rd to the 21st of January 1985 the average minimum temperature in the open barn was -12.6 degrees C and the lowest temperature was -19 degrees C, and in the period from the 8th to the 20th of February 1985 the average minimum temperature was -13.1 degrees C and the lowest recorded temperature was also -19 degrees C. At the beginning of hypothermic stress, the concentration of non-esterified acids and total proteins increased in the cows of the experimental group; potassiaemia was decreased in this group. Cholesterolaemia and glycaemia increased during the long-continued exposure to low temperatures. The activity of the thyroid gland was increased at all measurements.

[The effect of extreme temperatures on hematologic and biochemical indicators in heifers]. / Vplyv extrémnych teplôt prostredia na hematologické a biochemické ukazovatele u jalovíc.

The study was aimed at finding the effect of low and high temperatures on some biochemical parameters in heifers. Thirty half-sister heifers were used as the experimental material. The animals were divided into two groups. The experimental group including 16 heifers was kept loose on deep litter in an open barn with no thermal insulation. The control heifers (n = 14) were kept in a thermally insulated house. Blood was collected at extreme temperatures: above 30 degrees C and below -5 degrees C. An insignificant decrease of haemoglobin and haematocrit was recorded in the blood collected at low and high temperatures from the heifers kept in the open barn; a reduction of cholesterolaemia and total lipaemia was recorded at high temperatures. High and low temperatures significantly increased the concentration of free amino acids; the differences between the experimental and control group being from 36 to 76 mumol per litre. The content of 3.5-cyclic adenosine monophosphate was increased insignificantly at extreme temperatures. Hyperglycaemia was recorded in the heifers at high temperatures and at the beginning of the winter season. At low temperatures the concentration of thyroxine was found to be increased in the blood of the experimental animals: 120 nmol per litre and 161 nmol per litre vs. 95 nmol and 116 nmol in the control group.

[The effect of prolonged movement restriction of dairy cows on biochemical parameters]. / Vplyv dlhodobého obmedzenia pohybu dojníc na biochemické ukazovatele.

In thirty-three dairy cows--first calves, the influence of hypokinesis on biochemical parameters was studied over three lactation periods. The cows of the experimental group (n = 15) were reared in steel cages enabling only minimum movement, whereas the control group (n = 18) was housed loosely. It was found out that the permanent movement restriction led to a statistically significant decrease in hemoglobin and hematocrit concentrations, as well as in aspartate aminotransferase activity. In comparison with the control group, a significant decrease was also observed in substances of the lipid group--cholesterol, triglycerides and total lipids. Similarly, thyroxin and total proteinemia were lower.