RESUMO
INTRODUCTION: Quantitative reverse transcription polymerase chain reaction (RT-qPCR) can detect the severe acute respiratory syndrome Coronavirus-2 (SARS-CoV-2) in a highly specific manner. However, a decrease in the specificity of PCR assays for their targets may lead to false negative results. METHODS: Here, 177 high-coverage complete SARS-CoV-2 genome sequences from 13 Brazilian states were aligned with 15 WHO recommended PCR assays. RESULTS: Only 3 of the 15 completely aligned to all Brazilian sequences. Ten assays had mismatches in up to 3 sequences and two in many sequences. CONCLUSION: These results should be taken into consideration when using PCR-based diagnostics in Brazil.
Assuntos
COVID-19/virologia , Genoma Viral , SARS-CoV-2/genética , Brasil , Simulação por Computador , Humanos , Pandemias , RNA Viral/genética , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Sensibilidade e EspecificidadeRESUMO
Plants have developed mechanisms to avoid harmful effects of Na+ accumulation, such as the signaling pathway of carrier proteins Na+/H+ (NHX) and salt overly sensitive (SOS). Besides, endoplasmic reticulum (ER) could integrate plant cell response. Thus, we aimed to understand the effects of ER homeostasis impairment, and its relationship to salt stress during early stages of the Sorghum bicolor CSF 20 a salt-tolerant variety. Three days old seedlings were challenged with NaCl (0, 50, 75 and 100 mM), dithiothreitol (DTT) at 0, 2.5, 5.0 10.0 mM, and the combined NaCl and DTT treatments. Tunicamycin (TUN) was also used as a second inducer of ER stress in a quantitative PCR, to corroborate with DTT's results. There was no significant change in growth parameters under NaCl treatments. Nevertheless, seedling length, mass and Na+ content were decreased as DTT concentration was increased. Under combined NaCl and DTT treatments, shoot length and fresh and dry masses were maintained at control levels. On the other hand, the levels of Na+ were decreased, in comparison to NaCl treatment. Genes analyzed by qPCR revealed that NaCl was able to induce all of them, except for SbbZIP60, however it was induced under combined stresses. In conclusion, the results indicated that S. bicolor seedlings of CSF 20 variety were tolerant to salt and sensible to ER stress. The combination of both stresses restored the ER homeostasis promoting a decrease of Na+ content via the membrane transporters SbNHX1, SbSOS1, and SbPDI ER-chaperone and the ER sensor SbbZIP60.
Assuntos
Ditiotreitol , Estresse do Retículo Endoplasmático , Plântula , Cloreto de Sódio , Sorghum , Ditiotreitol/farmacologia , Estresse do Retículo Endoplasmático/efeitos dos fármacos , Plântula/efeitos dos fármacos , Cloreto de Sódio/farmacologia , Sorghum/efeitos dos fármacosRESUMO
Abstract INTRODUCTION: Quantitative reverse transcription polymerase chain reaction (RT-qPCR) can detect the severe acute respiratory syndrome Coronavirus-2 (SARS-CoV-2) in a highly specific manner. However, a decrease in the specificity of PCR assays for their targets may lead to false negative results. METHODS: Here, 177 high-coverage complete SARS-CoV-2 genome sequences from 13 Brazilian states were aligned with 15 WHO recommended PCR assays. RESULTS: Only 3 of the 15 completely aligned to all Brazilian sequences. Ten assays had mismatches in up to 3 sequences and two in many sequences. CONCLUSION: These results should be taken into consideration when using PCR-based diagnostics in Brazil.
