The relationship of average backfat thickness of feedlot steers to performance and relative efficiency of fat and protein retention.

Selection for growth and improved carcass merit has resulted in cattle that are variable in composition of gain during the finishing phase. This study assessed the relative performance among cattle with different levels of initial backfat thickness. It also exploited the ability to track carcass composition in the live animal with ultrasound estimates of backfat and marbling. A procedure was developed to partition and estimate relative efficiency of fat and protein gain. The trial periods were the last 43 or 50 d before slaughter and included 10 pens (average of 27 animals per pen) that ranged in average backfat thickness from 6.3 to 13.1 mm. There was no correlation (r2 = 0.0026) between average backfat thickness and G:F (g/kg of DMI). Correlations between average backfat thickness and ADG or DMI were also nearly zero (r2 = 0.0007 and 0.0042, respectively). Fat deposition from NEg was 3.98 times more efficient than protein deposition. Carcass backfat thickness was a poor predictor of carcass marbling score (r2 = 0.083), even though backfat thickness was an important predictor of the percentage of empty body fat (r2 = 0.807). The results indicate that a measure of backfat thickness on the live animal during the finishing phase is not an effective predictor of future feed efficiency. They also confirm that protein accretion is energetically expensive, and that using a single coefficient for predicting gain from NEg is valid regardless of whether gain is predominantly muscle or fat. These data document that there is little relationship between body composition and marbling score, which is contrary to models that assume a USDA quality grade target at a specified percent fat end point.

Using receiver operating characteristic analysis to evaluate the accuracy in predicting future quality grade from ultrasound marbling estimates on beef calves.

Ultrasound estimates of marbling score were collected on 144 calves that averaged 219 d of age and 219 kg. Those estimates were correlated (r2 = .32, P < .001) with carcass marbling at slaughter 252 d later. Receiver operating characteristic (ROC) analysis provides a method to determine critical operating points for selecting outcome groups with specified percentages of the desired quality grades. When an a priori estimate of the prevalence of Choice or premium choice can be made, the ROC procedures incorporate error rates to estimate the percentage of the herd that will be selected in an outcome group. A unique feature of the ROC output is the ability to conduct a cost/benefit analysis. The ROC analysis indicated that the initial marbling estimates were 78+/-4% accurate in classifying future quality grade and predicting whether an animal would grade USDA Choice. A similar accuracy (76+/-4%) was observed in predicting whether an animal would attain USDA Average Choice or higher (premium choice). A decision matrix was also examined in which sensitivity and specificity in predicting Choice were 90% and 46%, respectively. Relative values of those diagnostic measures were reversed in predicting premium choice (39% and 86%, respectively). Evaluation of the different methods indicated that ROC analysis may have advantages over either traditional regression analysis or contingency tables for evaluating ultrasound procedures.

Using serial ultrasound measures to generate models of marbling and backfat thickness changes in feedlot cattle.

Serial ultrasound estimates of backfat thickness and marbling score were obtained on two groups of steers fed high-energy rations for averages of 166 and 148 d. These measurements were used to develop mathematical models that described the changes in those two attributes as functions of days on feed. An exponential model best described the increase in backfat thickness, and the rate coefficients were .0117 and .0096 for the two groups (doubling times = 59 and 72 d, respectively). A modified power function was better than either a linear or exponential model for describing the development of marbling. In this model, marbling increased very slowly, approximately one degree every 100 d, in the interval from low Select to low Choice but then increased at faster rates as initial marbling scores became higher. Ultrasound backfat measures could be used to predict days to reach a target carcass backfat level with an average error of 30 d or less when backfat thickness averaged more than 3 mm at evaluation. Projections from measurements taken when cattle were started on feed were not accurate from one group, which averaged only 1.7 mm backfat at that time. Projections of carcass marbling improved as evaluation date neared slaughter date. Projections from the initial evaluation date allowed tentative categorization of candidates for Choice or not Choice but were only 64% accurate. Projections from evaluations made later in the feeding period exceeded 75% accuracy in distinguishing Choice from Select. Only small relationships (r2 = .07 or .16) existed between carcass backfat thickness and marbling score. Results indicate that ultrasound estimates of backfat and marbling made during the feeding period can be used to predict carcass merit at slaughter.

Influence of steroid implants and concentrate feeding on performance and carcass composition of cull beef cows.

Sixty nonpregnant, mature beef cows were used to evaluate the influence of steroid implants on performance and carcass composition of beef cows fed a high-concentrate diet. Twelve nonfed cows were slaughtered at 0 d. Remaining cows were stratified by weight and randomly assigned to an implant treatment and feeding period (28 or 56 d). Treatments included 1) no implant (control), 2) a 200-mg trenbolone acetate (TBA) implant, 3) a 200-mg testosterone propionate +20 mg of estradiol benzoate (TEB) implant, or 4) both implants (TBA + TEB). As days on feed increased, hot carcass weight (HCW), carcass soft tissue (CST) weight, adjusted fat thickness (AFT), longissimus muscle area (LMA), percentage of CST, and percentage of CST lipid increased (P < .05) and percentage of CST crude protein and moisture decreased (P < .05). Cows fed for 56 d accumulated (P < .05) more kilograms of lean than cows fed for 28 d, and cows fed for 28 d tended (P = .08) to have more kilograms of lean than nonfed cows. Cows fed for 56 d had (P < .05) higher dressing percentages than nonfed cows or cows fed for 28 d, and nonfed cows had (P < .05) higher dressing percentages than cows fed for 28 d. Implanted cows compared with control cows had (P < .05) heavier final weights, increased gains, improved feed efficiency, heavier hot carcass weights, larger LMA, higher cutability yield grades, compositionally leaner CST (higher percentage of moisture and protein and lower percentage of lipid), and more kilograms of lean. In addition, double-implanted (TBA + TEB) cows had (P < .05) more kilograms of CST than control and TBA-implanted cows. Furthermore, cows implanted with TBA had (P < .05) lower dressing percentages than TEB-implanted cows, less AFT than controls, and fewer kilograms of lipid than cows on the other three treatments. Full feeding of thin beef cows for 28 or 56 d increased carcass weight through an increase in CST lean as well as fat (lipid). The live performance and accretion of lean during realimentation can be enhanced by using anabolic implants.

Influence of steroid implants and concentrate feeding on carcass and longissimus muscle sensory and collagen characteristics of cull beef cows.

Sixty nonpregnant, mature beef cows were used to determine the effects of steroid implants and concentrate feeding on carcass quality, longissimus muscle (LM) collagen characteristics, and LM sensory traits. Twelve nonfed cows were slaughtered at 0 d to establish basal carcass values. The remaining 48 cows were assigned randomly in a 2 x 4 factorial arrangement to an implant treatment and fed for either 28 or 56 d. The implant treatments were 1) nonimplanted (controls), 2) a 200-mg trenbolone acetate (TBA) implant, 3) a 200-mg testosterone propionate +20 mg estradiol benzoate (TEB) implant, or 4) both implants (TBA+TEB). Carcasses from cows fed for 28 and 56 d had improved (P < .05) LM marbling, lean maturity, and quality grade; a lighter (P < .05) LM color (higher Hunter L* values); a higher (P < .05) percentage of LM soluble (heat-labile) collagen; and a lower (P < .05) LM Warner-Bratzler shear force value (more tender) than carcasses from nonfed cows. Feeding for 28 and 56 d also improved (P < .05) LM sensory panel traits of flavor intensity, connective tissue amount, myofibrillar tenderness, and overall tenderness. Feeding cows for an additional 28 d (to 56 d) improved (P < .05) LM visual lean color, texture, and firmness and carcass fat color. All LM HunterLab color measurements were higher (P < .05) for cows fed for 56 d compared to 28 d, indicating a brighter, redder, more vivid color. Implant treatments did not influence (P > .05) carcass quality or LM color. Steaks from implanted cows compared to controls had (P < .05) more soluble (heat-labile) collagen, a higher percentage of soluble collagen, and improved sensory traits of tenderness (myofibrillar and overall) and connective tissue amount. Steaks from TBA-implanted cows compared to the other implant treatments had superior (P < .05) LM sensory evaluations for myofibrillar and overall tenderness. Feeding thin cows a high-concentrate diet for 28 d improved quality grade and LM sensory traits, and feeding for 56 d improved LM lean and carcass fat color. Implanting fed cows improved LM sensory panel tenderness.

Estimating marbling score in live cattle from ultrasound images using pattern recognition and neural network procedures.

Neural network processing of texture statistics (which parameterized longissimus muscle echograms of live cattle) resulted in marbling estimates that differed from corresponding USDA carcass marbling scores by an average of .42 marbling score units. This was more accurate (P < .001) than using the same features in a multiple regression model. Images were used from 53 cattle in the training set and from 108 cattle in the validation set. Over 500 texture statistics (including variations in direction, resolution, and step size) were screened to identify three candidates (Markovian homogeneity--step size = one; third quadrant emphasis from the bit-4, normalized run length/gray level matrix; and 12-pixel local standard deviation) for intensive analysis. The differences between the live animal estimates and carcass marbling were not much greater than the human error in assigning carcass marbling scores. When the results were subjected to receiver operating characteristic analysis, accuracies in grade classification were comparable to clinical, diagnostic imaging evaluations. It is feasible to incorporate this procedure into a computer interfaced with an ultrasound system to provide unsupervised instrument evaluation of live cattle in "near real time" (2 or 3 s).

Evaluation of reproductive traits in Bos taurus and Bos indicus crossbred heifers: relationship of age at puberty to length of the postpartum interval to estrus.

Records of age at puberty (AAP) and duration of the postpartum interval to estrus (PPI) for heifers calving first at 2 yr of age were used to determine the relationship between the two reproductive traits. The study from which these records were obtained was designed in a 2 x 2 x 2 factorial arrangement of treatments. Angus x Hereford (AH; n = 148) and Brahman x Hereford (BH; n = 148) heifers were allotted within breed after weaning by weight into light (LW) and heavy (HW) weight blocks. Heifers were assigned by age to different levels of energy (low or high) in diets calculated to reach a target weight of 55% (LE) or 65% (HE) of their projected mature weight by the onset of the breeding period. Data were analyzed within breed and included only records for which both AAP and PPI were available. Pearson correlation coefficients for AAP to PPI were r = -.12 (P = .20) and r = .05 (P = .71) for AH and BH, respectively. Eliminating animals that experienced dystocia from the analyses yielded correlations of r = -.27 (P = .02) and r = .06 (P = .65) for Ah (n = 69) and BH (n = 51), respectively. When energy level, weight block, and energy level x weight block were included in the model for PPI, analyses of variance indicated that PPI among AH heifers was influenced most by the weight at which heifers began the trial after weaning (P = .01) but not by energy level (P = .23) or the interaction of energy level x weight block (P = .48).(ABSTRACT TRUNCATED AT 250 WORDS)

The repeatability and accuracy of ultrasound in measuring backfat of cattle.

Repeatability of ultrasound backfat measurements was determined on 217 cattle, and the association of ultrasound and carcass backfat measurements was evaluated with 580 animals. The correlation (repeatability) between consecutive ultrasound measurements was .975. Average difference between two ultrasound measures was .72 mm and error size was directly related (P less than .001) to the amount of backfat. Ultrasound measures averaged 8% (P less than .001) less than carcass measures. The average absolute difference between these two measures was 1.57 mm. Discrepancies were (P less than .001) larger when backfat was thicker; cattle with backfat measuring less than 10 mm averaged 1.43 mm absolute difference, whereas those with backfat greater than 10 mm averaged 1.89 mm. The variance of the discrepancies was partitioned into three components: ultrasound measurement error (14%), drift related to intercept and slope (15%), and residual (71%). The residual component may have represented errors in carcass measurement. In a subsequent trial with 175 cattle, average difference between ultrasound and carcass backfat measurements was reduced to 1.19 mm. Also, the slope and intercept virtually mimicked the isopach. Ultrasound measures can be more precise (minutely exact) and accurate (close to the true value) than carcass measures for assessing absolute backfat thickness in cattle. Elements of measurement protocol that may improve ultrasound technique are imparted in the manuscript.

Influence of dietary energy on follicular development, serum gonadotropins, and first postpartum ovulation in suckled beef cows.

Twenty-eight Hereford x Angus cows (4 yr of age) were used to determine the effects of pre- and postpartum dietary energy on performance and reproductive function in suckled beef cows. The experiment was designed as a 2 x 2 factorial with cows receiving either 70 (L) or 150% (H) of NRC recommended level of dietary energy before and(or) after parturition, resulting in four treatment combinations (L-L, L-H, H-L, H-H). Prepartum diets were fed for approximately 110 d, and postpartum diets were fed until either 10 d after the second postpartum ovulation or 150 d postpartum for those cows that failed to ovulate. Cows receiving low compared with high levels of energy before calving lost more (P less than .01) weight, body condition, subcutaneous fat, and longissimus muscle area before parturition and had calves with lighter (P less than .01) birth weights. Cows receiving low compared with high levels of energy postpartum lost more (P less than .01) weight, body condition, and longissimus muscle area after parturition. Low levels of energy before and after parturition decreased (P less than .01) milk production and calf weight at 70 d of age. Rates of cervical and uterine involution were unaffected by dietary energy treatments. Cows receiving high levels of energy prepartum had increased (P less than .01) mean concentrations and pulse frequency of LH in serum after parturition, and cows receiving high levels of energy postpartum had increased (P less than .05) pulse frequency of LH. Low levels of energy postpartum decreased (P less than .01) appearance rate of small (5.0 to 7.9 mm) and large (greater than or equal to 10 mm) follicles, and low levels of energy prepartum decreased (P less than .02) appearance rate of large follicles based on transrectal ultrasonography. Cows receiving high levels of energy prepartum had shorter (P less than .02) intervals from parturition to ovulation, and a higher (P less than .01) percentage of the cows that received high levels of energy postpartum ovulated by 150 d postpartum. In summary, prepartum level of dietary energy influenced birth weight and weight gain of calves, milk production, concentrations and pulse frequency of LH in serum, appearance rate of large follicles, and the interval to first ovulation. Postpartum level of dietary energy influenced milk production, weight gain of calves, pulse frequency of LH, appearance rate of small and large follicles, and the percentage of cows that ovulated after parturition.

Evaluation of reproductive traits in Bos taurus and Bos indicus crossbred heifers: effects of postweaning energy manipulation.

Reproductive traits were evaluated in Bos taurus and Bos indicus crossbred heifers that were fed different diets during the postweaning period. The study was designed in a 2 x 2 x 2 factorial arrangement of treatments. Angus x Hereford (AH; n = 148) and Brahman x Hereford (BH; n = 148) heifers were sorted after weaning by body weight into light (LW) and heavy (HW) weight blocks. Heifers in each weight block were assigned to diets calculated to reach a target weight of 55% (LE) or 65% (HE) of their projected mature weights by the start of the breeding season. Puberty was determined after a 160-d observation period and characterized by the following criteria: 1) behavioral estrus, 2) presence of a palpable corpus luteum (d 6 to 10; estrus = d 0), and 3) rise in serum progesterone above 1 ng/ml (d 6 to 10). A higher (P = .01) proportion of AH heifers than of BH heifers reached puberty by the breeding season (93% vs 67%). Interactions of breed x weight block and energy level x weight block also contributed to this difference. Weight at puberty was heavier (P = .001) among HE than among LE heifers and greater for heifers in HW than for those in LW blocks (P = .02). Differences in prebreeding weight, body condition, average daily gain, hip height, and pelvic area were influenced selectively by breed, energy level, or weight block. Pregnancy rates were higher (P = .01) among AH than among BH heifers. Incidence and severity of dystocia was influenced by the breed x energy level interaction (P = .01). Brahman x Hereford heifers had less (P = .01) dystocia than AH heifers, HE heifers had less (P less than .02) dystocia than LE heifers, and HE-AH heifers had less (P less than .01) dystocia than LE-AH contemporaries. Subsequent duration of the postpartum interval to estrus was shorter (P = .002) among AH than among BH females. Pregnancy rates at the end of the 2nd yr were higher (P = .02) among LW than among HW females and weights were heavier (P = .001) at weaning among calves weaned from BH dams.

Relationship of ultrasound speckle to marbling score in cattle.

A subjective, but repeatable, scoring system was devised to quantitate ultrasound speckle in tomograms of the longissimus muscle over the 12th rib. Speckle scores in 11 of 14 groups of cattle (n = 619) were highly correlated (P less than .001) with carcass marbling score. Live animal speckle scores classified carcasses as Select or Choice with 77% accuracy. Similar accuracy was achieved as much as 148 d before slaughter by adjusting for the regression of speckle scores with days on feed. Marbling scores were not distributed normally with both positive skewness and kurtosis (P less than .001). The procedure exploited an unmodified low-cost ultrasound system; scores were estimated instantly from the monitor image. This ability to predict carcass grade at distant dates might be incorporated into a strategy to cluster feedlot cattle into outcome groups for more effective marketing. The results suggest that mathematical analysis of the ultrasound radio frequency signal or pixel map statistics might reveal parameters that could improve accuracy and precision of the method and, conceivably, could predict specific attributes of beef palatability.

Performance, carcass, cartilage calcium, sensory and collagen traits of longissimus muscles of open versus 30-month-old heifers that produced one calf.

One hundred eleven Simmental x Hereford (3/8 to 5/8 Simmental) heifers were used to determine the effects of age, parturition and implantation on performance, carcass and meat-sensory traits, muscle-collagen characteristics and thoracic-button calcification. Eighty-five heifers that calved at about 2 yr of age, designated as single-calf heifers (SCH), were either implanted (I-SCH) with Synovex-H or not implanted (NI-SCH). The remaining 26, 2-yr-old non-pregnant heifers (2-OH) served as controls. Additionally, 24, 1-yr-old open heifers (1-OH) from the same genetic source were utilized as the standard heifer-production system. The 1-OH and 2-OH were slaughtered after being fed a high-grain diet for 137 and 112 d, respectively. The SCH were fed the same high-grain diet beginning about 1 mo after calving and were fed 137 d before slaughter. The 33 I-SCH were implanted when started on the high-grain diet. Calves were weaned about 5 wk before the SCH were slaughtered. The 2-OH had the highest (P less than .05) feedlot ADG, whereas no differences (P greater than .05) occurred among other treatments. Dressing percentages were higher (P less than .01) for I-SCH than for NI-SCH. Carcass weights were lowest (P less than .05) and percentage kidney, pelvic and heart fat was highest (P less than .01) for 1-OH. Fat thickness, yield grades, marbling scores and quality grades were similar (P greater than .05) and desirable for all treatments.(ABSTRACT TRUNCATED AT 250 WORDS)

Response of prepubertal Bos taurus and Bos indicus x Bos taurus heifers to melengestrol acetate with or without gonadotropin-releasing hormone.

The effectiveness of treatments to induce estrus in prepubertal beef heifers was evaluated. Angus x Hereford (n = 148) and Brahman x Hereford (n = 148) heifers were sorted after weaning by body weight into light and heavy weight blocks. Heifers were assigned to diets, calculated to reach a target weight of 55% or 65% of their projected mature weight by the start of breeding. Cyclicity was determined after a 160-d observation period and from concentrations of progesterone in serum determined 10 d before and on the day that treatments began to induce puberty. The remaining nonpubertal heifers, with concentrations of progesterone in serum of less than 1 ng/ml (0 or 10 d before treatment), were assigned randomly within breed and nutrition group to either a melengestrol acetate + saline (MGA+S) or MGA + gonadotropin-releasing hormone (MGA+GnRH) treatment. Prepubertal Angus x Hereford heifers (n = 11) and Brahman x Hereford heifers (n = 49) were fed 0.5 mg MGA for 7 d. Forty-eight hours after MGA, heifers were injected with 500 ug s.c. GnRH or 5 ml of saline. Blood samples were collected from all prepubertal heifers every 3 d after GnRH or saline for 30 d. There was no difference between treatments in the proportion of heifers that exhibited estrus by Day 7 after treatment. However, a larger (P<0.05) proportion of MGA+S-treated heifers exhibited estrus within 14 d after treatment than MGA+GnRH-treated heifers (87 vs 63%). Among heifers that exhibited estrus during that time period, the proportion with increased progesterone was higher (P<0.10) for the MGA+GnRH group than for the MGA+S group (71 vs 41%, Day 7; 79 vs 54%, Day 14). There was no difference in conception rate at first service between treatment groups. Thirty-seven and 53%, respectively, of the MGA+S and MGA+GnRH-treated heifers had short estrous cycles after treatment, and 44 and 50%, respectively, of those short cycles were repeated. Pregnancy rates at the end of 45 d were numerically higher for MGA+S heifers than for MGA+GnRH treated counterparts (63 vs 53%).

Conception rate in Bos taurus and Bos indicus crossbred heifers after postweaning energy manipulation and synchronization of estrus with melengestrol acetate and fenprostalene.

Conception rate in heifers after synchronization of estrus with melengestrol acetate (MGA) and fenprostalene (a prostaglandin F2 alpha analogue; PGF) was determined in pubertal Bos taurus and Bos indicus crossbred yearling heifers. Angus x Hereford (AH, n = 137) and Brahman x Hereford (BH, n = 97) heifers were sorted by body weight after weaning into light (LW) and heavy (HW) weight blocks. Heifers were assigned by age to diets to reach a target weight of 55% (LE) or 65% (HE) of their projected mature weight by the start of breeding. Heifers that exhibited estrus and had serum progesterone greater than or equal to 1 ng/ml (0 or 10 d before estrous synchronization treatment) were assigned randomly within breed and nutritional groups to either an estrous synchronization (S) or control (C) group. Heifers in the S group were fed .5 mg of MGA for 7 d and injected s.c. with 2 mg PGF on d 7 of MGA. All heifers were inseminated 12 h after first detected estrus. A greater proportion of AH (P less than .01) than of BH heifers were in estrus within 6 d after PGF, and more S heifers than C heifers (P less than .01) were in estrus. Conception rate at first service was proportionately higher (P less than .001) in AH than in BH heifers and lower (P less than .02) in S than in C heifers. There was a breed x energy level interaction (P less than .01) for conception rate at first service. Stage of the estrous cycle at the time treatment with MGA was initiated influenced (P less than .05) conception rate at first service in the S, AH heifers, with lower conception rates among heifers beginning treatment late in their estrous cycles (greater than or equal to d 12). Pregnancy rates after 21 d were higher (P less than .01) in AH than in BH heifers and higher (P less than .01) in HW than in LW heifers. More HE than LE heifers (P less than .02), and more AH than BH heifers were pregnant after 45 d. Pregnancy rates at the end of 21 d were higher among HE, BH heifers than among LE contemporaries. A higher (P less than .02) percentage of HE, HW, BH heifers were pregnant at the end of 45 d compared with other BH groups. Results indicated that a 7-d MGA-PGF treatment reduced conception rates at first service in pubertal yearling heifers. Pregnancy rate was affected by prebreeding nutrition in BH yearling heifers at the end of 45 d.

Control of horn flies on cattle with insecticide pellets fired from a CO2-operated pistol.

A method is needed to control pyrethroid-resistant horn flies (Haematobia irritans) which would involve the intermittent application of insecticides to cattle without their confinement for treatment. Our objective was to test a CO2 gas-operated pistol, designed to shoot marking pellets, to determine the number of pellet applications required to obtain season-long control. Also, since horn fly resistance to pyrethroid insecticides is widespread, we tested ten other insecticides as concentrates in pellets. Six pellet pistol applications of 38% permethrin provided 84% control of horn flies for 15 weeks. Single pellet applications of ten non-pyrethroid insecticides indicated that none of these insecticides, at the concentrations tested, were as effective as permethrin for control of pyrethroid-resistant horn flies. Our field evaluation of the pellet-pistol method indicates that it has sufficient merit to justify further testing for horn fly control.

Clinical evaluation of insecticide-impregnated tags attached to chains and used as backrubbers for control of Haematobia irritans.

Fenvalerate and permethrin insecticide tags were tested for horn fly (Haemotobia irritans) control. Three fenvalerate-impregnated ear tags attached to a chain and used as a backrubber controlled horn flies on steers for 8 weeks, and six tags provided 18 weeks control. Ten tags impregnated with permethrin failed to control horn flies. The choice of insecticide in tags attached to chains and used as backrubbers for horn fly control may be important, as fenvalerate and permethrin (both pyrethroids) varied in effectiveness.