Processing of the FMRFamide precursor protein in the snail Lymnaea stagnalis: characterization and neuronal localization of a novel peptide, 'SEEPLY'.

In the pulmonate snail Lymnaea stagnalis, FMRFamide-like neuropeptides are encoded by a multi-exon genomic locus which is subject to regulation at the level of mRNA splicing. We aim to understand the post-translational processing of one resulting protein precursor encoding the tetrapeptide FMRFamide and a number of other putative peptides, and determine the distribution of the final peptide products in the central nervous system (CNS) and periphery of Lymnaea. We focused on two previously unknown peptide sequences predicted by molecular cloning to be encoded in the tetrapeptide protein precursor consecutively, separated by the tetrabasic cleavage site RKRR. Here we report the isolation and structural characterization of a novel non-FMRFamide-like peptide, the 22 amino acid peptide SEQPDVDDYLRDVVLQSEEPLY. The novel peptide is colocalized with FMRFamide in the CNS in a number of identified neuronal systems and their peripheral motor targets, as determined by in situ hybridization and immunocytochemistry. Its detection in heart excitatory motoneurons and in nerve fibres of the heart indicated that the novel peptide may play a role, together with FMRFamide, in heart regulation in the snail. The second predicted peptide, STEAGGQSEEMTHRTA (16 amino acids), was at very low abundance in the CNS and was only occasionally detected. Our current findings, suggestive of a distinct pattern of post-translational processing, allowed the reassessment of a previously proposed hypothesis that the two equivalent sequences in the Aplysia FMRFamide gene constitute a molluscan homologue of vertebrate corticotrophin releasing factor-like peptides.

A life-sized physical model of the human cochlea with optical holographic readout.

A life-sized physical model of the human cochlea is demonstrated. The model consists of two fluid-filled chambers separated by a polymer membrane and connected through a small hole that serves the same functional purpose as the helicotrema. The dimensions of the two chambers were made identical to those of scala vestibuli and scala tympani in the real cochlea. The membrane's width and length are the same as the values measured for the biological basilar membranes, and its thickness is chosen to give it appropriate rigidity. A piezoelectric transducer drives the system via an elastic window (acting as the oval window) on one side of the scala vestibuli. The resulting vibration pattern on the basilar membrane is investigated with an optical novelty filter, with detection sensitivity of 0.3 A at 2 kHz with a 1-Hz bandwidth. The overall response of this model is found to be a good extrapolation of Bekesy's low-frequency data. However, the tuning curves of this model are not as sharp as those found by Rhode, Johnstone, and others from the in vivo measurements. Possible implications of these results are discussed.

Renal tumors in young adults.

Primary renal malignancies are relatively rare in young adults. Eighteen patients between the ages of twenty and forty years underwent nephrectomy for renal tumors at our institution between 1947 and 1989. Data were reviewed in this group regarding method of presentation, duration of symptoms, tumor histology, pathologic stage, and survival. Tumor histology parallels that of older patients, the majority being adenocarcinoma (78%). The duration of symptoms prior to seeking medical attention was long, averaging eighty-four weeks. The overall survival rate, excluding nontumor deaths, was 50 percent. No patient with nodal or distant metastases survived, regardless of histology.