[Crystallographic picture of the gingival fluid in healthy teeth and by inflammatory periodontal disease].

The research revealed crystallographic structure of the gingival fluid in healthy teeth and by inflammatory periodontal disease. Comparing the results with clinical data both crystallographic patterns in healthy teeth gingival fluid and markers of periodontal tissue pathology were established.

[Myocardial revascularization in correction of electrical instability of the heart in patients with postinfarction cardiosclerosis].

We have assessed parameters of myocardial electrical instability in patients with postinfarction atherosclerosis before and in one year after coronary bypass surgery. Inclusion criteria were history of myocardial infarction, surgical myocardial revascularization between 1 and 2 years before; stable course of coronary heart disease during previous month; preserved ejection fraction ( more or equal 50%), stable sinus rhythm. Heterogeneity of myocardial electrophysiological properties has diminished after coronary bypass surgery due to improvement of coronary perfusion and restoration of function of ischemic myocardium. This have been associated with positive dynamics of interval QT dispersion, parameters of turbulence and variability of cardiac rhythm, reduction of the rate of registration of ventricular late potentials.

[Influence of laser radiation of the whole blood in vitro on adhesion and aggregation of blood platelets].

The authors revealed dependence of reaction blood plates to photoeffect on the dose and rate of blood movement at laser radiation of donor blood in vitro. The red light decreases adhesion and aggregation of blood plates both at high and low rate of shift. Infrared laser radiation is effective only at high rate of shift leading to increase of adhesion and decrease of aggregation of blood plates. Blue laser is effective in small doses only and at low rate of sift it leads to decrease of adhesion and at high rate it provokes increase of adhesion. Blue laser do not have a significant influence on aggregation of blood plates. These results make possible to suppose ambiguity of biological response of venous and arterial blood to radiation.

[Nitric oxide in the lymphatic microvessel regulation]. / Uchastie oksida azota v reguliatsii funktsii limfaticheskikh mikrososudov.

Topical application of sodium nitroprusside on rat mesentery has a marked influence on lymph microvessels function. The drug causes a dilation of majority of lymphangions and decrease of the pacemaker activity of the vessel wall structures and valves. These changes do not lead to lymph stasis, and lymph flow velocity does not reduce. The non-selective inhibitor of NO synthase (N-nitro-L-arginine) intensifies vasomotions of lymph microvessels, modulates phasic contractile activity and increases lymph flow velocity. There is a time dependent dynamic of changes in action of N-nitro-L-arginine. During inhibition of endogenous NO synthesis the responses of lymph microvessels on sodium nitroprusside application are modified: the constriction of majority lymphangions and activation of valve work are observed.

Changes in lymph microcirculation during pathological stress.

Stress caused by immobilization and acoustic stimulation produces considerable changes in lymph microcirculation. These changes manifested in dilation of microvessels, enhanced phasic contractile activity and its abnormal pattern, accelerated lymph flow, and recruitment of new lymphangions in drainage functions.

[Functional organization of lymphatic microvessels of the rat mesentery]. / Funltsional'naia organizatsiia limfaticheskikh mikrososudov bryzheiki krysy.

Lymph flow as measured with biomicroscopic and speckle-interferometrical techniques was very unstable and its rate did not depend on lymphangion diameter in the rat mesentery. A correlation was found between amount of cells in the lymph flow and other indices of lymphangion functions. All microvessels with phasic contractions and/or working valve were revealed as having a lymph flow. The phasic activity and the valve work correlated with each other. The higher the amplitude and frequency of the phasic activity and the longer the contraction cycle, the higher was the rate of the valve work.

Adherence properties of Staphylococcus aureus under static and flow conditions: roles of agr and sar loci, platelets, and plasma ligands.

Global regulatory genes in Staphylococcus aureus, including agr and sar, are known to regulate the expression of multiple virulence factors, including cell wall adhesins. In the present study, the adherence of S. aureus RN6390 (wild type), RN6911 (agr), ALC136 (sar), and ALC135 (agr sar) to immobilized fibrinogen, fibronectin, von Willebrand factor (vWF), extracellular matrix (ECM), and human endothelial cells (EC) EAhy.926 was studied. Bacteria grown to postexponential phase were subjected to light oscillation (static condition) or to shear stress at 200 s(-1) (flow condition) on tissue culture polystyrene plates coated with either protein ligands, ECM, or EC. Adherence of nonlabeled bacteria to immobilized ligands was measured by an image analysis system, while adherence of [(3)H]thymidine-labeled S. aureus to ECM and EC was measured by a beta-scintillation counter. The results showed increased adherence of agr and agr sar mutants to immobilized fibrinogen and higher potential of these mutants to induce platelet aggregation in suspension, decreased adherence of sar and agr sar mutants to immobilized fibronectin and vWF as well as to ECM and EC, increased adherence of both S. aureus wild type and sar mutant to EC treated with platelet-rich plasma (PRP) compared to platelet-poor plasma (PPP) and to EC treated with PPP compared to the control, and increased adherence of S. aureus wild type to EC coated with PRP in which platelets were activated with phorbol 12-myristate 13-acetate compared to intact PRP. This finding paralleled the increased adherence to EC of activated compared to intact platelets. It is suggested that platelet-mediated S. aureus adherence to EC depends on platelet activation and the number of adherent platelets and available receptors on the platelet membrane. In conclusion, the agr locus downregulates S. aureus adherence to fibrinogen, while the sar locus upregulates S. aureus adherence to fibronectin, vWF, ECM, and EC. The effect of both agr and sar on S. aureus adherence properties develops primarily under flow conditions, which suggests different adhesion mechanisms in static and flow conditions.

Blood irradiation by He-Ne laser induces a decrease in platelet responses to physiological agonists and an increase in platelet cyclic GMP.

The effect of He-Ne laser irradiation on platelet adhesion, activation and aggregation was investigated. Citrated whole blood was irradiated in vitro by He-Ne laser (632.8 nm, 7 mW) and then subjected to shear stress (1300 s-1) on subendothelial extracellular matrix (ECM)-coated plates. Laser irradiation was followed by a decrease in platelet adhesion and aggregation on ECM under flow conditions in a time exposure-dependent manner (by 30-40%). The inhibiting effect of laser light on platelets was detectable up to 1 h after the termination of irradiation. Laser irradiation of either platelet-rich plasma, gel-filtered platelets, platelet-poor plasma, or packed blood cells followed by whole blood reconstitution revealed a marked decrease in platelet deposition on ECM only in the cases of platelet-rich plasma or gel filtered platelets. In conventional aggregometry, laser-treated platelet-rich plasma demonstrated a diminished platelet response to both thrombin receptor-activating peptide (TRAP), converting a two-wave aggregation curve to reversible, and to the protein kinase C activator PMA (by 45%). In flow cytometry analysis, irradiated platelets presented lower fibrinogen binding and P-selectin expression in response to TRAP. Laser irradiation had no additional inhibitory effect on dibutyryl cGMP- and dibutyryl cAMP-pretreated platelets. A 50% increase in cGMP level was observed in laser-treated gel filtered platelets, both in the presence and in absence of the phosphodiesterase inhibitor, isobuthylmethylxanthine. The results suggest that guanylate cyclase is one of the primary mediators of the laser effect on platelet function.

[The effect of dimethyl sulfoxide on the changes in the lymph microcirculation induced by staphylococcal toxin]. / Vliianie dimetilsul'foksida na izmeneniia limfomikrotsirkuliatsii, vyzvannye stafilokokkovym toksinom.

The effect of dimethyl sulfoxide (dimexid, DMSO) on the lymph microvessels of the rat mesentery was studied by vital biomicroscopy. It was found that 30% DMSO increases the tonus of intact lymph microvessels and stimulates their phase contractions. Action of the agent for more than 15 min leads to the development of lymphostasis. DMSO has a pronounced corrective effect on disorders of lymph microcirculation caused by the staphylococcal toxin. It removes the main lymphopathogenic effects of the poison and normalizes the drainage function of the lymphangions. The correcting effect of DMSO on lymph circulation is attended with attenuation of the fatal effect of the toxin.

[Mechanism of disorders of the cerebral blood supply during stress in spontaneously hypertensive rats]. / K mekhanizmu narusheniia krovosnabzheniia golovnogo mozga pri stresse u spontanno gipertenzivnykh krys.

The immobilization- and sound stimulus-induced changes in cerebral circulation were studied in spontaneously hypertensive rats. It was found that there was a stress-induced increase in the levels of epinephrine and norepinephrine in brain tissue, which was associated with lower levels of vasoactive prostaglandins E and F2 and with their imbalance with vasodilator prostaglandins E being prevalent. The change in the cerebral ratio of biologically active substances results in cerebral blood flow autoregulation failure and development of large cerebral hemorrhages.

[Sex differences in adrenocortical sensitivity and resistance to cerebrovascular damage in rats under strong stress]. / Polovye razlichiia adrenokortikal'noi chuvstvitel'nosti i ustoichivosti k tserebrovaskuliarnym povrezhdeniiam u krys pri sil'nom stresse.

Dynamics of changes in adrenal and plasma corticosterone and the development of cerebrovascular lesions were studied in both male and female rats, exposed to strong stress (combined immobilization and intermittent found sound for 2 hours). Plasma corticosterone levels in stressed females were 460% and 660% of the control values when measured on stress minute 10 and 120. The corresponding values in male rats were 220% and 360%. The stress-induced dilatation of brain vessels and the increases in vascular permeability were less pronounced in females than in males, when studied 0.1 and 24 hours after termination of stress. The number of brain perivascular haemorrhages was markedly reduced in females compared with males. It is supposed that higher resistance to stress-induced cerebrovascular lesions in females may be attributed to higher functional reserves of steroidogenesis.

[Effect of staphylococcal toxin on the microcirculatory system]. / Vliianie stafilokokkovogo toksina na sistemu mikrotsirkuliatsii.

Study of microcirculation in the mesentery of narcotized rats showed that the staphylococcal toxin causes increased migration of leukocytes, aggregation of formed elements, and disturbance of the blood flow in the small venules and arterioles. The toxin stimulates rhythmic contractile activity of the lymphatic microvessels and induces their constriction and even complete obliteration. The lymphoconstrictive effect of the toxin is weakened by verapamil and completely removed by dimethyl sulfoxide.

[Effect of sodium oxybutyrate on the development of a staphylococcal intoxication]. / Vliianie natriia oksibutirata na razvitie stafilokokkovoi intoksikatsii.

Sodium oxybutyrate (200 and 400 mg/kg) was found to increase life expectancy of the animals poisoned with staphylococcus toxin. The improvement of the blood supply to the bulbar structure of the brain as well as normalization of hemorheological parameters probably underlie the protective effect of the drug.