RESUMO
Prostatic cancer (PC) stands out in terms of its occurrence, pathophysiology, and unfavorable prognostics in humans and dogs. Natural drugs bear an integrative potential for conventional antineoplastic treatments. In this context, the bioproducts of Synadenium grantii have been empirically used in different parts of Brazil for the integrative treatment of prostate cancer in humans. However, there is no availability of scientific evidence of the antitumor effects of S. grantii. Therefore, this study aimed to investigate the bioactive compounds in the latex of S. grantii using the high-resolution mass spectrophotometry (HRMS) and to evaluate its cytotoxic effects on primary canine PC cell cultures. Four fragments of phorbol ester were identified as potential bioactive compounds using the HRMS. With the help of an MTT ([3-(4,5-dimethyldiazol-2-yl)-2,5 diphenyltetrazolium bromide]) assay, two canine prostatic carcinoma cell lines (PC 1 and PC2) showed a decrease in the tumor cell count, with an Inhibitory concentration 50 (IC50)of 0.8469 and 0.6068 mg/ml, respectively, for PC1 and PC2. In conclusion, the latex of S. grantii contains phorbol esters in its composition, and its aqueous solution has a cytotoxic effect on canine metastatic PC cells in vitro.
RESUMO
O hemangiossarcoma é a neoplasia maligna originária do endotélio vascular. Os sítios primários mais comuns são baço, fígado, coração e pele, sendo considerado raro o acometimento da pálpebra e demais componentes oculares. Neste relato, descreve-se o caso de uma cadela da raça Boxer, de dez anos de idade, que apresentou aumento de volume, na borda livre da terceira pálpebra, de cor vermelho escuro. O tratamento de escolha foi a remoção cirúrgica e o diagnóstico de hemangiossarcoma foi confirmado ao exame histopatológico.(AU)
Hemangiosarcoma is malignant neoplasm originating from the vascular endothelium. The most common primary sites of this neoplasia are the spleen, liver, heart and skin. The involvement of the eyelid and other ocular components is unusual. In this report, we describe the case of a 10-year-old Boxer bitch who presented an increase in volume, at the free edge of the third eyelid, a deep red color. The mass was surgically removed and the diagnosis of hemangiossarcoma was confirmed by histopathological exam.(AU)
El hemangiosarcoma es una neoplasia maligna originada en el endotelio vascular, encontrado principalmente en el bazo, hígado, corazón y piel. Su presencia en párpados y otros componentes oculares es poco común. En este relato se describe el caso de una perra de la raza Boxer, de diez años, que presentó aumento de volumen, en el borde libre del tercer párpado, de color rojo oscuro. El tratamiento escogido fue la remoción quirúrgica y el diagnóstico de hemangiosarcoma fue confirmado por examen histopatológico.(AU)
