Microbacterium plantarum sp. nov. and Microbacterium thalli sp. nov., two endophytic metal-resistant bacteria isolated from Sphaeralcea angustifolia (Cav.) G. Don and Prosopis laevigata (Humb. et Bonpl. ex Willd) M.C. Johnston.

Four Gram-positive, aerobic, catalase- and oxidase-negative, rod-shaped, motile endophytic bacterial strains, designated NM3R9T, NE1TT3, NE2TL11 and NE2HP2T, were isolated from the inner tissues (leaf and stem) of Sphaeralcea angustifolia and roots of Prosopis laevigata. They were characterized using a polyphasic approach, which revealed that they represent two novel Microbacterium species. Phylogenetic analysis based on 16S rRNA gene sequencing showed that the species closest to NE2HP2T was Microbacterium arborescens DSM 20754T (99.6 %) and that closest to NM3R9T, NE2TL11 and NE2TT3 was Microbacterium oleivorans NBRC 103075T (97.4 %). The whole-genome average nucleotide identity value between strain NM3R9T and Microbacterium imperiale DSM 20530T was 90.91 %, and that between strain NE2HP2T and M. arborecens DSM 20754T was 91.03 %. Digital DNA-DNA hybridization showed values of less than 70 % with the type strains of related species. The polar lipids present in both strains included diphosphatidylglycerol, phosphatidylglycerol, glycolipids and unidentified lipids, whereas the major fatty acids included anteiso-C15 : 0, anteiso-C17 : 0, iso-C16 : 0 and C16 : 0. Whole-cell sugars included mannose, rhamnose and galactose. Strains NM3R9T and NE2HP2T showed physiological characteristics different from those present in closely related Microbacterium species. According to the taxonomic analysis, both strains belong to two novel species. The name Microbacterium plantarum sp. nov. is proposed for strain NE2HP2T (=LMG 30875T=CCBAU 101117T) and Microbacterium thalli sp. nov. for strains NM3R9T (=LMG 30873T=CCBAU 101116T), NE1TT3 (=CCBAU 101114) and NE2TL11 (=CCBAU 101115).

New Insights on Dietary Polyphenols for the Management of Oxidative Stress and Neuroinflammation in Diabetic Retinopathy.

Diabetic retinopathy (DR) is a neurodegenerative and vascular pathology that is considered one of the leading causes of blindness worldwide, resulting from complications of advanced diabetes mellitus (DM). Current therapies consist of protocols aiming to alleviate the existing clinical signs associated with microvascular alterations limited to the advanced disease stages. In response to the low resolution and limitations of the DR treatment, there is an urgent need to develop more effective alternative therapies to optimize glycemic, vascular, and neuronal parameters, including the reduction in the cellular damage promoted by inflammation and oxidative stress. Recent evidence has shown that dietary polyphenols reduce oxidative and inflammatory parameters of various diseases by modulating multiple cell signaling pathways and gene expression, contributing to the improvement of several chronic diseases, including metabolic and neurodegenerative diseases. However, despite the growing evidence for the bioactivities of phenolic compounds, there is still a lack of data, especially from human studies, on the therapeutic potential of these substances. This review aims to comprehensively describe and clarify the effects of dietary phenolic compounds on the pathophysiological mechanisms involved in DR, especially those of oxidative and inflammatory nature, through evidence from experimental studies. Finally, the review highlights the potential of dietary phenolic compounds as a prophylactic and therapeutic strategy and the need for further clinical studies approaching the efficacy of these substances in DR management.

Mycobiota of Mexican Maize Landraces with Auxin-Producing Yeasts That Improve Plant Growth and Root Development.

Compared to agrochemicals, bioinoculants based on plant microbiomes are a sustainable option for increasing crop yields and soil fertility. From the Mexican maize landrace "Raza cónico" (red and blue varieties), we identified yeasts and evaluated in vitro their ability to promote plant growth. Auxin production was detected from yeast isolates and confirmed using Arabidopsis thaliana plants. Inoculation tests were performed on maize, and morphological parameters were measured. Eighty-seven yeast strains were obtained (50 from blue corn and 37 from red corn). These were associated with three families of Ascomycota (Dothideaceae, Debaryomycetaceae, and Metschnikowiaceae) and five families of Basidiomycota (Sporidiobolaceae, Filobasidiaceae, Piskurozymaceae, Tremellaceae, and Rhynchogastremataceae), and, in turn, distributed in 10 genera (Clavispora, Rhodotorula, Papiliotrema, Candida, Suhomyces, Soliccocozyma, Saitozyma Holtermaniella, Naganishia, and Aeurobasidium). We identified strains that solubilized phosphate and produced siderophores, proteases, pectinases, and cellulases but did not produce amylases. Solicoccozyma sp. RY31, C. lusitaniae Y11, R. glutinis Y23, and Naganishia sp. Y52 produced auxins from L-Trp (11.9-52 µg/mL) and root exudates (1.3-22.5 µg/mL). Furthermore, they stimulated the root development of A. thaliana. Inoculation of auxin-producing yeasts caused a 1.5-fold increase in maize plant height, fresh weight, and root length compared to uninoculated controls. Overall, maize landraces harbor plant growth-promoting yeasts and have the potential for use as agricultural biofertilizers.

The Role of Cannabinoids in CNS Development: Focus on Proliferation and Cell Death.

The active principles of Cannabis sativa are potential treatments for several diseases, such as pain, seizures and anorexia. With the increase in the use of cannabis for medicinal purposes, a more careful assessment of the possible impacts on embryonic development becomes necessary. Surveys indicate that approximately 3.9% of pregnant women use cannabis in a recreational and/or medicinal manner. However, although the literature has already described the presence of endocannabinoid system components since the early stages of CNS development, many of their physiological effects during this stage have not yet been established. Moreover, it is still uncertain how the endocannabinoid system can be altered in terms of cell proliferation and cell fate, neural migration, neural differentiation, synaptogenesis and particularly cell death. In relation to cell death in the CNS, knowledge about the effects of cannabinoids is scarce. Thus, the present work aims to review the role of the endocannabinoid system in different aspects of CNS development and discuss possible side effects or even opportunities for treating some conditions in the development of this tissue.

Distribuição espacial dos casos de Zika vírus em um estado do Nordeste Brasileiro / Distribución espacial de casos de virus Zika en un estado del Noreste de Brasil / Spatial distribution of Zika virus cases in a Northeast Brazilian state

Objetivo: Analisar a distribuição espacial dos casos de Zika vírus no Piauí entre 2015 e 2019. Método: Tratou-se de um estudo ecológico espacial da Zika vírus, com dados do Sistema de Informação de Agravos de Notificação (SINAN )de 2015 a 2019. Foi aplicado o teste de associação X2 para a análise bivariada e para análise espacial foi utilizado o software Qgis (versão3.16.7). Resultados: Foram notificados 1354 casos e 207 casos confirmados da doença. O município de Teresina (51,33%) e a região de saúde Entre Rios (42%) apresentaram os maiores registros. O sexo feminino (72,30%), a faixa entre 20 e 34 anos (40,03%), etnia parda (65,88%) e escolaridade ignorada (43,21%) foram os mais afetados. Conclusão: A identificação do perfil epidemiológico da Zika é uma forma de auxiliar o sistema de saúde de todo o estado na elaboração de políticas de controle específicas para as populações mais vulneráveis.(AU)

Objective:To Analyze The spatial distribution of Zika virus cases in Piauí between 2015 and 2019. Method: Thiswas a spatialecologicalstudyoftheZikavirus, with data fromtheNotifiableDiseasesInformation System (SINAN) from 2015 to 2019. The X2 association test was applied for thebivariateanalysisandtheQgis software (version 3.16.7) was used for spatial analysis. Results:1354 cases and 207 confirmed cases of the disease were reported. The municipality of Teresina (51.33%) and the health region Entre Rios (42%) had the highest records. Females (72.30%), aged between 20 and 34 years (40.03%), mixed race (65.88%) andunknowneducation (43.21%) were the most affected.Conclusion: The epidemiological profile ofZikais a way to help the health system of the entire state in the elaboration of specific control policies for the most vulnerable populations.(AU)

Objetivo:Analizar La Distribución espacial de los casos de virus Zika Piauí entre 2015 y 2019. Método:Este fue un estudio ecológico espacial del virus Zika, con datos del Sistema de Información de Enfermedades de Declaración Obligatoria SINAN) de 2015 a 2019.Para el análisis bivariado se aplicó la prueba de asociación X2 y para el análisis espacial se utilizó el software Qgis (versión 3.16.7). Resultados:Se reportaron 1354 casos y 207 casos confirmados de la enfermedad. La ciudad de Teresina (51,33%) y laregiónsanitaria Entre Ríos (42%) tuvieronlos registros más altos.El sexo femenino (72,30%), conedad entre 20 y 34 años (40,03%), mestizo (65,88%) y sinescolaridad (43,21%) fueronlos más afectados.Conclusíon: La identificacióndel perfil epidemiológico delZika es una forma de ayudar al sistema de salud de todo el estado enlaelaboración de políticas de control específicas para laspoblaciones más vulnerables.(AU)

Retinal Toxicity Induced by Chemical Agents.

Vision is an important sense for humans, and visual impairment/blindness has a huge impact in daily life. The retina is a nervous tissue that is essential for visual processing since it possesses light sensors (photoreceptors) and performs a pre-processing of visual information. Thus, retinal cell dysfunction or degeneration affects visual ability and several general aspects of the day-to-day of a person's lives. The retina has a blood-retinal barrier, which protects the tissue from a wide range of molecules or microorganisms. However, several agents, coming from systemic pathways, reach the retina and influence its function and survival. Pesticides are still used worldwide for agriculture, contaminating food with substances that could reach the retina. Natural products have also been used for therapeutic purposes and are another group of substances that can get to the retina. Finally, a wide number of medicines administered for different diseases can also affect the retina. The present review aimed to gather recent information about the hazard of these products to the retina, which could be used to encourage the search for more healthy, suitable, or less risky agents.

Evaluation of the prematurity retinopathy and other eye changes in the newborn.

OBJECTIVE: To assess the prevalence of ophthalmologic manifestations in newborns in a maternity hospital in the city of São Paulo, SP, and the main risk factors related with the development of retinopathy of prematurity. METHODS: A retrospective, longitudinal study with patients born from 2015 to 2017 who required ophthalmological evaluation. The research variables were obtained by analysis of the newborn medical charts. RESULTS: A total of 773 patients were studied. The sample consisted of 288 examinations performed by indication of gestational age ≤32 weeks: 118 (42.4%) in 2015, 105 (42.2%) in 2016, 65 (26.4%) cases in 2017. There were 329 evaluations indicated due to birth weight: 113 (40.6%) in 2015, 108 (43.4%) in 2016, and 108 (43.9%) in 2017. The prevalence of associated risk factors was 97 (34.9%) cases in 2015, 96 (38.6%) in 2016, and 54 (22%) in 2017, followed by mechanical ventilation with 82 (29.5%) cases in 2015, 64 (25.7%) in 2016 and 41 (16.7%) in 2017, and continuous positive airway pressure with 59 (21.2%) cases in 2015, 72 (28.9%) in 2016, and 46 (18.7%) in 2017. For the other indications, the evaluations performed due to congenital syphilis were the majority in the 3-year period of the study, with 55 (19.8%) newborns in 2015, 54 (21.7%) in 2016, and 59 (24.0%) in 2017. The most prevalent ophthalmologic diagnosis was retinopathy of prematurity, with 79 (35.3%) cases in 2015, 64 (32.2%) in 2016, and 41 (24.1%) in 2017. CONCLUSION: Most neonates born in the organization do not present risk factors for ophthalmological manifestations. Retinopathy of prematurity was the disease with greater strength of association found in our study. For the other indications, the evaluations performed due to congenital syphilis prevail in the 3- year period of the study.

The Production and Evaluation of an Electrochemical Sensors for Strychnine and Its Main Metabolite Strychnine N-Oxide for Their Use in Biological Samples.

Strychnine (STN) and its major metabolite Strychnine N-Oxide (SNO) were examined electrochemically. Both parent compounds and its major metabolite showed electroactivity on glassy carbon electrodes using CV and DPV techniques. One oxidation peak at 1008 mV was observed for STN with the optimum peak intensity at pH 7. SNO produced two oxidation peaks, at 617 mV and 797 mV, at pH 5. The peaks demonstrated irreversible behaviour and the irreversibility of the system was confirmed at different scan rates. A calibration curve was produced for both CV and DPV measurements and the sensitivity of the proposed EC method was good compared with previous electrochemical and non-electrochemical methods. The precision of oxidation peak of STN using the STN-MIP method produced a maximum value of 11.5% and 2.32% for inter-day and intraday %RSD, respectively. The average% recovery was around 92%. The electrochemical method has been successfully applied to the determination of STN in spiked plasma and urine samples. For SNO, both anodic peaks of SNO demonstrated irreversible behaviour. A different sweep rate was used for calculating the number of 'transfer electrons' in the system; based on this, the mechanism of oxidation reaction was proposed. Calibration curves for both oxidative peaks were produced using DPV measurements. The second anodic peak demonstrated high linearity and precision with %RSD < 1.96%.

Lack of professional recognition: main reason for stress in military police.

Introduction: Military police officers play a crucial role in contemporary society, which is marked by the increase in criminality. Therefore, these professionals are constantly under pressure, both socially and professionally, so occupational stress is something present in their routine. Objectives: To investigate the stress levels of military police officers in the municipality of Fortaleza and its metropolitan region. Methods: This was a cross-sectional, quantitative study, conducted with 325 military police officers (53.1% men; 20> 51 years old) who belonged to military police battalions. The Police Stress Questionnaire was used to identify the stress level, following the Likert scale from 1 to 7; the higher the score, the higher the stress level. Results: The results indicated that the lack of professional recognition is the main stress factor among military police officers (Median = 7.00). Other items were relevant to the quality of life of these professionals, which are: "risks of injuries or wounds resulting from the profession", "working on days off", "lack of human resources", "excessive bureaucracy in the police service", " having the perception that we are pressured to give up free time "," lawsuits resulting from police service," "going to court, relationship with the judicial actors, " and "use of inadequate equipment for the service," respectively (Median = 6. 00). Conclusions: The stress of these professionals is organizational in nature and comes from factors that transcend the violence with which they deal.

Introdução: O policial militar desempenha uma função de suma relevância na sociedade contemporânea, a qual é marcada pelo avanço da criminalidade. Desse modo, esse profissional se encontra constantemente sob pressão, tanto social quanto trabalhista, de maneira que o estresse ocupacional é algo presente em sua rotina. Objetivos: Investigar os níveis de estresse em policiais militares da cidade de Fortaleza e região metropolitana. Métodos: Trata-se de um estudo transversal, de natureza quantitativa, realizado com 325 policiais militares, sendo (53,1% do sexo masculino; 20 > 51 anos de idade) pertencentes aos batalhões de policiamento militar. O questionário Police Stress Questionnaire foi utilizado para identificar o nível de estresse, seguindo a escala Likert de 1 a 7 - quanto maior a pontuação, maior o nível de estresse. Resultados: Os resultados indicaram que a falta de reconhecimento profissional é o principal fator de estresse entre os policiais militares (mediana = 7,00). Outros itens foram relevantes na qualidade de vida desses profissionais, são eles: "riscos de lesões ou ferimentos resultantes da profissão", "trabalhar em dias de folga", "falta de recursos humanos", "excesso de burocracia no serviço policial", "ter a percepção que somos pressionados a abdicar o tempo livre", "processos de justiça decorrentes do serviço policial", "idas ao tribunal, relação com os intervenientes judiciais" e "uso de equipamentos inadequado para o serviço" respectivamente (mediana = 6,00). Conclusões: O estresse desses profissionais é de cunho organizacional e provém de fatores que transcendem à violência com a qual lidam.

Evaluation of the prematurity retinopathy and other eye changes in the newborn

ABSTRACT Objective To assess the prevalence of ophthalmologic manifestations in newborns in a maternity hospital in the city of São Paulo, SP, and the main risk factors related with the development of retinopathy of prematurity. Methods A retrospective, longitudinal study with patients born from 2015 to 2017 who required ophthalmological evaluation. The research variables were obtained by analysis of the newborn medical charts. Results A total of 773 patients were studied. The sample consisted of 288 examinations performed by indication of gestational age ≤32 weeks: 118 (42.4%) in 2015, 105 (42.2%) in 2016, 65 (26.4%) cases in 2017. There were 329 evaluations indicated due to birth weight: 113 (40.6%) in 2015, 108 (43.4%) in 2016, and 108 (43.9%) in 2017. The prevalence of associated risk factors was 97 (34.9%) cases in 2015, 96 (38.6%) in 2016, and 54 (22%) in 2017, followed by mechanical ventilation with 82 (29.5%) cases in 2015, 64 (25.7%) in 2016 and 41 (16.7%) in 2017, and continuous positive airway pressure with 59 (21.2%) cases in 2015, 72 (28.9%) in 2016, and 46 (18.7%) in 2017. For the other indications, the evaluations performed due to congenital syphilis were the majority in the 3-year period of the study, with 55 (19.8%) newborns in 2015, 54 (21.7%) in 2016, and 59 (24.0%) in 2017. The most prevalent ophthalmologic diagnosis was retinopathy of prematurity, with 79 (35.3%) cases in 2015, 64 (32.2%) in 2016, and 41 (24.1%) in 2017. Conclusion Most neonates born in the organization do not present risk factors for ophthalmological manifestations. Retinopathy of prematurity was the disease with greater strength of association found in our study. For the other indications, the evaluations performed due to congenital syphilis prevail in the 3- year period of the study.

Functional characterization of culturable fungi from microbiomes of the "conical cobs" Mexican maize (Zea mays L.) landrace.

Mexican maize landraces, produced for local consumption, are adapted to different environmental conditions, and their yield is affected by abiotic and biotic factors, including the use of agrochemicals. The search for sustainable alternatives to agrochemicals includes the study of the culturable microbial communities. In this study, the fungal communities associated with 2 Mexican maize landraces reddish and bluish "conical cobs" were found to be comprised of Ascomycota fungi, represented by 89 strains within 6 orders (Pleosporales, Hypocreales, Onygenales, Capnodiales, Helotiales, and Eurotiales) and 16 genera. Cellulases and metallophores production were the primary enzymatic products and plant growth-promoting activities were detected among the isolates. Penicillium, Didymella, and Fusarium strains had the most active enzymatic and plant growth promoting activities, however, Aspergillus sp. HES2-2.2, Talaromyces sp. RS1-7, and Penicillium sp. HFS3-3 showed antagonistic activity against the four phytopathogenic Fusarium strains Fusarium oxysporum, Fusarium sambucinum, Fusarium fujikuroi and Fusarium incarnatum-equiseti and also a high and diverse production of enzymatic and plant growth promoting activities; here we identified fungal strains as candidates to promote maize growth.

Contribuições do programa mais médicos no enfrentamento da pandemia de covid-19 na Bahia / Contributions of the mais médicos program in the fight against the covid-19 pandemic in the state of Bahia / Contribuciones del programa mais médicos en la lucha contra la pandemia del covid-19 en Bahía

O Programa Mais Médicos (PMM) faz parte do pacto de melhoria do atendimento aos usuários do Sistema Único de Saúde (SUS). Visa atender as regiões de maior vulnerabilidade social do Brasil e suprir a ausência de profissionais médicos na Atenção Básica (AB). Com o PMM, a Bahia aumentou a média de médicos por habitantes, beneficiando cerca de 5,6 milhões de pessoas. O surgimento da Covid-19 impôs a adoção de medidas sanitárias e de ações estratégicas que motivaram mudanças nos fluxos e na readequação estrutural das Equipes de Saúde da Família. Buscando qualificar esses profissionais, foi desenvolvido na modalidade de Educação a Distância (EAD) o curso de capacitação Manejo Clínico da Covid-19 para Médicos do PMM. Este artigo tem como objetivo relatar o processo de elaboração e implementação de tal curso no formato de um relato de experiência da execução. Foi realizada a análise dos produtos das webreuniões de supervisões longitudinais, do conteúdo dos vídeos e do manual de recomendação para o enfrentamento da Covid-19. O curso apresentou protocolos de atendimento da Covid-19 na AB, articulados em três módulos, com videoaulas produzidas por médicos. Foram capacitados 365 médicos do PMM e apoiadores da AB do estado, que ampliaram as ações para os 417 municípios da Bahia por meio dos coordenadores da AB. As contribuições do PMM no contexto da pandemia da Covid-19 no estado da Bahia estão sendo significativas na perspectiva de qualificação profissional e aperfeiçoamento dos serviços da AB no atendimento da população.

The Mais Médicos Program (MMP) is part of the pact to improve service to users of the Brazilian Unified Health System (SUS). It aims to serve the regions of greatest social vulnerability in Brazil and reduce the absence of health professionals in the territory. With the MMP, the state of Bahia increased the number of physicians per inhabitants, benefiting around 5.6 million people. The emergence of Covid-19 imposed the adoption of sanitary measures and strategic actions that motivated changes in the flows and structural readjustment of the Family Health Teams. Seeking these professionals, the Training Course Clinical Management of Covid-19 for Doctors of the PMM was developed in Distance Education (Distance Education). This article seeks to report the process of elaboration and implementation if the course. For such purpose, the experience of running the course is described. The products of the web meetings of longitudinal supervisions, the content of the videos and the recommendation manual to face Covid-19 were analyzed. The course presents Covid-19 service protocols in Primary Care (PC). It took place in 3 modules with video classes produced by doctors. In total, 365 doctors from the MMP and the supporters of PC in the state were trained and expanded to the 417 municipalities of the state of Bahia by the PC coordinators. The contributions of the MMP in the context of the Covid-19 pandemic in the state of Bahia are being relevant in the perspective of professional qualification and improvement of primary care services in serving the population.

El Programa Mais Médicos (PMM) forma parte del pacto para mejorar el servicio a los usuarios del Sistema Único de Salud (SUS). Su objetivo es atender las regiones de mayor vulnerabilidad social en Brasil y reducir la ausencia de personal médico en la atención primaria (AP). Con el PMM, Bahía aumentó el número de médicos por habitante, beneficiando a cerca de 5,6 millones de personas. El surgimiento del Covid-19 impuso la adopción de medidas sanitarias y acciones estratégicas que motivaron cambios en los flujos y reajustes estructurales de los Equipos de Salud de la Familia. Con el fin de capacitar a estos profesionales, se desarrolló el curso de capacitación: Manejo Clínico del Covid-19 para Doctores del PMM en la modalidad a distancia. Este artículo tuvo como objetivo informar el proceso de elaboración e implementación del curso a partir del informe de experiencia del curso. Se realizó el análisis de los productos de las reuniones web de supervisión longitudinal, así como el contenido de los videos y el manual de recomendaciones para enfrentar al Covid-19. El curso presentó los protocolos de atención al Covid-19 en la AP, desarrollados en tres módulos con videoclases producidas por médicos. Se capacitaron a 365 médicos del PMM y simpatizantes de AP en el estado, que han expandido la acción a los 417 municipios de Bahía mediante los coordinadores de AP. Los aportes del PMM en el contexto de la pandemia del Covid-19 en el estado de Bahía están siendo relevantes en la perspectiva de la calificación profesional y mejora de los servicios de AP al servicio de la población.

Functional genomics of a Spiroplasma associated with the carmine cochineals Dactylopius coccus and Dactylopius opuntiae.

BACKGROUND: Spiroplasma is a widely distributed endosymbiont of insects, arthropods, and plants. In insects, Spiroplasma colonizes the gut, hemolymph, and reproductive organs of the host. Previous metagenomic surveys of the domesticated carmine cochineal Dactylopius coccus and the wild cochineal D. opuntiae reported sequences of Spiroplasma associated with these insects. However, there is no analysis of the genomic capabilities and the interaction of this Spiroplasma with Dactylopius. RESULTS: Here we present three Spiroplasma genomes independently recovered from metagenomes of adult males and females of D. coccus, from two different populations, as well as from adult females of D. opuntiae. Single-copy gene analysis showed that these genomes were > 92% complete. Phylogenomic analyses classified these genomes as new members of Spiroplasma ixodetis. Comparative genome analysis indicated that they exhibit fewer genes involved in amino acid and carbon catabolism compared to other spiroplasmas. Moreover, virulence factor-encoding genes (i.e., glpO, spaid and rip2) were found incomplete in these S. ixodetis genomes. We also detected an enrichment of genes encoding the type IV secretion system (T4SS) in S. ixodetis genomes of Dactylopius. A metratranscriptomic analysis of D. coccus showed that some of these T4SS genes (i.e., traG, virB4 and virD4) in addition to the superoxide dismutase sodA of S. ixodetis were overexpressed in the ovaries. CONCLUSION: The symbiont S. ixodetis is a new member of the bacterial community of D. coccus and D. opuntiae. The recovery of incomplete virulence factor-encoding genes in S. ixodetis of Dactylopius suggests that this bacterium is a non-pathogenic symbiont. A high number of genes encoding the T4SS, in the S. ixodetis genomes and the overexpression of these genes in the ovary and hemolymph of the host suggest that S. ixodetis use the T4SS to interact with the Dactylopius cells. Moreover, the transcriptional differences of S. ixodetis among the gut, hemolymph and ovary tissues of D. coccus indicate that this bacterium can respond and adapt to the different conditions (e.g., oxidative stress) present within the host. All this evidence proposes that there is a strong interaction and molecular signaling in the symbiosis between S. ixodetis and the carmine cochineal Dactylopius.

Edible Fruit Plant Species in the Amazon Forest Rely Mostly on Bees and Beetles as Pollinators.

Edible fruit plants of tropical forests are important for the subsistence of traditional communities. Understanding the most important pollinators related to fruit and seed production of these plants is a necessary step to protect their pollination service and assure the food security of these communities. However, there are many important knowledge gaps related to floral biology and pollination in megadiverse tropical rainforests, such as the Amazon Forest, due mainly to the high number of plant species. Our study aims to indicate the main pollinators of edible plants (mainly fruits) of the Amazon forest. For this, we adopted a threefold strategy: we built a list of edible plant species, determined the pollination syndrome of each species, and performed a review on the scientific literature searching for their pollinator/visitors. The list of plant species was determined from two specialized publications on Amazon fruit plants, totaling 188 species. The pollination syndrome was determined for 161 species. The syndromes most frequently found among the analyzed species were melittophily (bee pollination), which was found in 101 of the analyzed plant species (54%) and cantharophily (beetle pollination; 26 species; 14%). We also found 238 pollinator/visitor taxa quoted for 52 (28%) plant species in previous publications, with 124 taxa belonging to Apidae family (bees; 52%), mainly from Meliponini tribe (58 taxa; 47%). Knowledge about pollinators is an important step to help on preserving their ecosystem services and maintaining the productivity of fruit trees in the Amazon.

Detection of Common Deletional of α-Thalassemia 3.7 Kb from Metropolitan Region of Manaus, Amazonas, Brazil.

BACKGROUND: Alpha Thalassemia (α-thal) is a heterogeneous group of hereditary alterations caused by deletions that affect alpha regulatory genes, and the 3.7Kb deletion is the most frequent worldwide. The prevalence ranges from 20% and 35% in Brazil, depending mainly on race, predominant in Afro-descendants. PURPOSE: The aim was to determine α-thal -α3.7Kb and -α4.2Kb deletions, estimating their frequency in individuals from six regions of Amazonas State. METHODS: Volunteers age between 18-59 years old of both genders participated in the study. Blood was collected from March 2014 to September 2017 at the health centers of each participant city. α-thal3.7Kb was performed by GAP-PCR, while α-thal4.2Kb by Multiplex-PCR. The total samples collected from each city were: Manaus (capital), 356 (19.7%); Iranduba 232 (12.8%); Manacapuru, 287 (15.9%); Presidente Figueiredo, 370 (20.5%); Itacoatiara, 301 (16.6%); and Coari, 263 (14.5%). RESULTS: The average age among males was 35.3±14.8, while for females, it was 36.7±14.9 years old. Microcytosis (MCV <80fL) was found in 158 individuals (8,46%) and α-thal diagnosed in 143 individuals (7.9%), and all of these individuals carried the 3.7Kb deletion 5.95% in heterozygous and 1.95% in homozygous. α-thal4.2kb was not found in any volunteer. The association analyses to the α-thal3.7kb genotypes were statistically significant for all hematological parameters (p<.001), except serum iron and serum ferritin analyses. CONCLUSION: This study highlights α-thal 3.7kb deletion as an important public health problem, especially in a population not yet characterized about this disease. Thus, epidemiological studies using molecular tools become relevant in regions where the disease is underestimated, contributing to a better understanding of thalassemia incidence and iron deficiency anemias incidence of the participating cities. We reinforce that future molecular studies in North Region from Brazil can be utilized to describe other genetic anemias as structural hemoglobinopathies that have already proven to be highly prevalent in Brazil.

We and herbivores eat endophytes.

Health depends on the diet and a vegetal diet promotes health by providing fibres, vitamins and diverse metabolites. Remarkably, plants may also provide microbes. Fungi and bacteria that reside inside plant tissues (endophytes) seem better protected to survive digestion; thus, we investigated the reported evidence on the endophytic origin of some members of the gut microbiota in animals such as panda, koala, rabbits and tortoises and several herbivore insects. Data examined here showed that some members of the herbivore gut microbiota are common plant microbes, which derived to become stable microbiota in some cases. Endophytes may contribute to plant fibre or antimetabolite degradation and synthesis of metabolites with the plethora of enzymatic activities that they display; some may have practical applications, for example, Lactobacillus plantarum found in the intestinal tract, plants and in fermented food is used as a probiotic that may defend animals against bacterial and viral infections as other endophytic-enteric bacteria do. Clostridium that is an endophyte and a gut bacterium has remarkable capabilities to degrade cellulose by having cellulosomes that may be considered the most efficient nanomachines. Cellulose degradation is a challenge in animal digestion and for biofuel production. Other endophytic-enteric bacteria may have cellulases, pectinases, xylanases, tannases, proteases, nitrogenases and other enzymatic capabilities that may be attractive for biotechnological developments, indeed many endophytes are used to promote plant growth. Here, a cycle of endophytic-enteric-soil-endophytic microbes is proposed which has relevance for health and comprises the fate of animal faeces as natural microbial inoculants for plants that constitute bacterial sources for animal guts.

Size and isolation of naturally isolated habitats do not affect plant-bee interactions: A case study of ferruginous outcrops within the eastern Amazon forest.

Pollination may be severely affected by the decreasing size and increasing isolation of habitat patches. However, most studies that have considered the effects of these two variables on plant-pollinator interactions have been carried out in areas that have undergone anthropogenic fragmentation, and little is known about their effects in natural habitats. The Carajás National Forest and Campos Ferruginosos National Park are two protected areas in the eastern Amazon where one can find isolated ferruginous outcrops characterized by iron-rich soil and herbaceous-shrub vegetation surrounded by Amazon forest. These patches of canga provide an opportunity to analyze plant-pollinator interactions in naturally fragmented areas. Our objective was to test whether the size and isolation of naturally isolated outcrops located in Carajás affect plant-pollinator interactions by using pollination syndromes and interaction networks. We determined the pollination syndromes of 771 plant species that occurred in eleven canga patches and performed field work to analyze plant-pollinator networks in nine canga patches. The structure of the plant-pollinator networks was not affected by the size or isolation of the canga patches. Generalist species were present in all canga areas, indicating that they are important in maintaining the plant communities in isolated canga patches. The lack of significance related to the distance between canga patches suggests that the forest does not prevent pollinator movement between canga patches.

Apuntes históricos de la práctica de la Oftalmología en Matanzas / Historical notes about Ophthalmology' practice in Matanzas

Se narran los sucesos históricos más relevantes de la práctica de la especialidad de Oftalmología en la provincia de Matanzas y los relacionados con la formación de profesionales a partir de la década del 70 del siglo XX. Al utilizar el método de la narración histórica, se propició la reflexión sobre los propios acontecimientos y su relación con el contexto socio-económico al momento en que estos se produjeron; destacándolos como los antecedes y factores favorecedores del desarrollo de la Oftalmología en Matanzas. Este trabajo se realizó no sólo para narrar de manera cronológica la historia desde los inicios y creciente práctica local de la especialidad, sino también, como a manera de testimonio para las actuales y futuras generaciones, de cómo tributaron los profesionales, las instituciones y los propios eventos, al avance científico y al impacto social de la especialidad en Matanzas. Se concluye, que el comienzo del esplendor de la práctica de la Oftalmología en la provincia de Matanzas, se enmarca a la década del 70 del siglo pasado; y a los inicios del siglo XXI al de mayor desarrollo acumulado. Todo este salto de calidad en el tiempo y en el espacio, ha sido posible, gracias a las políticas de salud y educacionales implementadas por el Ministerio de Salud Pública del país (AU).

The authors recount the most relevant historical facts of the Ophthalmology practice in the province of Matanzas and those related to professionals´ training from the seventies of the XX century. Using the method of historical recount helped the reflection on the proper facts and their relation to the socio-economic context at the moment they took place, highlighting them as antecedents and factor favoring the development of Ophthalmology in Matanzas. The current paper was written not only to recount in a chronological way the history from the beginning and growing local practice of the specialty, but also as a testimony for the current and future generations to know how the professionals, institutions and events contributed to the scientific advance and to the social impact of the specialty in Matanzas. It is concluded that the beginning of the splendor of Ophthalmology practice in the province of Matanzas falls within the decade of the seventies of the past century, and the beginning of the XXI century is the time of the bigger accumulated development. All this quality jump in the time and space has been possible thanks to health and educational politics implemented by the Ministry og Public Health of the country (AU).