Combined exposure to electrochemical lysis and photodynamic therapy.

A combination of electrochemical lysis and photodynamic therapy were used to attain complete resorption of M-1 sarcoma in rats; both treatment modalities were used with minimum parameters. Fotolon served as the photosensitizer for photodynamic therapy. Accumulation of the sensitizer in the tumor and normal tissue was evaluated before photodynamic therapy. Complete resorption of sarcoma in 100% cases (vs. photodynamic monotherapy) was attained only by the following treatment protocol: fotolon injection 50 min before electrochemical lysis (10 min) followed by photodynamic therapy. No tumor tissue was detected in morphological sections.

Evaluation of antitumor efficiency of electrochemical lysis on the model of M-1 sarcoma.

Antitumor efficiency of electrochemical lysis was evaluated on the model of M-1 sarcoma. At stage 1 of the study, the results of therapy with electrodes in different position were compared, at stage 2 various combination of electrochemical lysis parameters (current strength and duration of exposure) were evaluated. The increase in parameters was associated with the increase in the percentage of cases with complete regression of tumors, which was confirmed by morphological data.