RESUMO
Ten children receiving 5 to 6 week courses of radiotherapy after brain tumor surgery were given ondansetron treatment for persistent nausea and emesis. All patients continued the ondansetron treatment until the end of their radiotherapy course. Nausea, emesis, appetite, and adverse events were scored throughout the ondansetron treatment period. Ondansetron was well tolerated by all patients and was effective at reducing symptoms in 60% of the children.
Assuntos
Antieméticos/uso terapêutico , Neoplasias Encefálicas/radioterapia , Ondansetron/uso terapêutico , Radioterapia/efeitos adversos , Adolescente , Antieméticos/efeitos adversos , Apetite , Astrocitoma/radioterapia , Neoplasias Cerebelares/radioterapia , Criança , Pré-Escolar , Ependimoma/radioterapia , Feminino , Germinoma/radioterapia , Humanos , Masculino , Meduloblastoma/radioterapia , Náusea/prevenção & controle , Ondansetron/efeitos adversos , Projetos Piloto , Vômito/prevenção & controleRESUMO
Two methods for the detection of membrane components in human stroma-free hemoglobin solutions are described. The first is a phospholipid assay with a detection limit of 0.5-1 nmol phospholipid/ml hemoglobin-solution. For the detection of membrane proteins an immunoassay with a monoclonal antibody against glycophorin alpha was developed (detection limit 0.01% of the original amount). These methods were used to determine the purity of Hb solutions prepared in two different ways. Hb solutions prepared by filtration of red blood cells, gradually swollen in hypotonic buffer, contained 0.25% of the original amount of phospholipid and no detectable glycophorin alpha. For Hb solutions prepared in a similar way from red blood cells lysed in water, the values for phospholipid and glycophorin alpha were 2.5% and 0.06%, respectively. The determination of both glycophorin alpha and phospholipid gives a useful indication of the purity of Hb solutions.
Assuntos
Substitutos Sanguíneos/química , Membrana Eritrocítica/química , Hemoglobinas/química , Proteínas de Membrana/análise , Anticorpos Monoclonais , Glicoforinas/análise , Humanos , Imunoensaio , Fosfolipídeos/análiseRESUMO
Two methods for the detection of membrane components in human stroma-free hemoglobin (SFHb) solutions are described. The first method is a phospholipid assay with a detection limit of 0.5-1 nmol phospholipid/ml SFHb. For the detection of membrane proteins an immunoassay with a monoclonal antibody against glycophorin alpha was developed (detection limit 0.01% of the original amount). The determination of both glycophorin alpha and phospholipid yields useful information on the purity of SFHb solutions, as was shown by determination of the purity of two SFHb solutions prepared in different ways.
Assuntos
Substitutos Sanguíneos/isolamento & purificação , Hemoglobinas/isolamento & purificação , Contaminação de Medicamentos , Membrana Eritrocítica/química , Glicoforinas/isolamento & purificação , Humanos , Lipídeos de Membrana/isolamento & purificação , Fosfolipídeos/isolamento & purificação , SoluçõesRESUMO
A specific carbonic anhydrase activity inhibitor (methazolamide) was injected into one vitreous body each of 4 New Zealand White rabbits. Electroretinograms (ERG) were recorded before and several times after the methazolamide injection. The stimulus levels maximized the rod and cone response characteristics of the rabbit ERG. The effects of methazolamide were followed over 5 h. During this time, the electroretinograms showed a decline in amplitude of both a and b waves at both stimulus levels. The data support the involvement of carbonic anhydrase in the excitatory physiological events in the retina. Preliminary evidence indicates a slow recovery of the carbonic-anhydrase-inhibited ERG.
