eNOS phosphorylation and translocation are altered in male but not female mice by increased activation of the Gαq protein.

Little is known about sex-dependent physiological and pathophysiological differences in cardiac endothelial nitric oxide synthase (eNOS) expression and activation. Therefore, we investigated cardiac morphology and eNOS protein expression, including its translocation-dependent activation and phosphorylation, in cardiac tissue of male and female wild-type mice and transgenic heart-failure mice having a cardiac-specific, 5-fold overexpression of the Galphaq protein. In addition, we measured calcineurin protein expression. Heart-to-body weight ratio was increased in Galphaq mice. Female wild-type mice showed higher eNOS protein expression and activation (translocation and phosphorylation) than did wild-type males. In cardiac tissue of Galphaq mice, these sex-dependent differences remained or were enhanced. Protein expression of the catalytic subunit calcineurin A, which has been shown to dephosphorylate eNOS, was higher in wild-type males than in wild-type females. These differences were increased in the Galphaq mice model. We conclude that sex differences exist in cardiac eNOS protein expression and phosphorylation. Increased activation of the Galphaq protein appears to alter eNOS protein expression and phosphorylation only in males.

Pathogenicity of Mycoplasma lipofaciens strain ML64 for turkey embryos.

Mycoplasma lipofaciens strain ML64, isolated from an egg of a northern goshawk (Accipiter gentilis), has been found to be pathogenic for chicken embryos causing mortality during the first 2 weeks of incubation. The same strain was inoculated in turkey embryos to evaluate its pathogenicity and its ability to be transmitted laterally in the hatchery. The strain was found to be pathogenic for turkey embryos, causing a high mortality (88.9%) during late incubation as well as haemorrhages of the legs, dwarfing, curled toes and a severe, multifocal, purulent to necrotizing bronchopneumonia. In addition, lateral transmission between turkey poults hatched from infected eggs and poults from non-infected controls was observed in the incubator.

Pathogenicity of Mycoplasma lipofaciens strain ML64, isolated from an egg of a Northern Goshawk (Accipiter gentilis), for chicken embryos.

Some Mycoplasma species are well-known avian pathogens and are of importance in poultry breeder flocks due to their pathogenic potential for embryos. Mycoplasmas are regularly detected in birds of prey, and a strain of Mycoplasma lipofaciens that was isolated from an egg of a Northern Goshawk (Accipiter gentilis) was examined for its pathogenicity in specific pathogen free chicken embryos since birds of prey eggs were not available for this purpose. The strain was found to be pathogenic, causing a high mortality as well as dwarfing, curled toes and infiltrations of heterophils in the liver, kidney, intestine and chorioallantoic membrane.