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1.
Virology ; 328(2): 233-43, 2004 Oct 25.
Artigo em Inglês | MEDLINE | ID: mdl-15464843

RESUMO

The proinflammatory cytokine tumor necrosis factor-alpha (TNF-alpha) inhibits normal keratinocytes proliferation. However, many human papillomavirus (HPV)-immortalized or transformed cell lines are resistant to TNF-alpha antiproliferative effect. The present study analyzes the effects of TNF-alpha on organotypic cultures of primary human keratinocytes (PHKs) that express HPV-18 oncogenes. Raft cultures prepared with PHKs acutely transfected with HPV-18 whole genome or infected with recombinant retroviruses containing only E6/E7 or E7 were treated with 2 nM TNF-alpha. While BrdU incorporation into basal/parabasal cells of normal PHKs cultures was markedly inhibited by TNF-alpha cultures transfected with HPV-18 whole genome showed proliferation in all cell strata. Furthermore, BrdU incorporation into cultures expressing E6/E7 or E7 was not significantly reduced, indicating that E7 alone confers partial resistance to TNF-alpha. Besides, TNF-alpha treatment did not alter p16ink4a, p21cip1, p27kip1, or cyclin E levels, but did reduce cyclin A and PCNA levels in sensitive cells.


Assuntos
Transformação Celular Viral/efeitos dos fármacos , Queratinócitos/virologia , Papillomaviridae/patogenicidade , Fator de Necrose Tumoral alfa/farmacologia , Ciclina A/metabolismo , Proteínas de Ligação a DNA/biossíntese , Proteínas de Ligação a DNA/genética , Humanos , Queratinócitos/patologia , Proteínas Oncogênicas Virais/biossíntese , Proteínas Oncogênicas Virais/genética , Papillomaviridae/genética , Antígeno Nuclear de Célula em Proliferação/metabolismo , Fase S
2.
In. Nathanson, Neal; Ahmed, Rafi; Gonzalez-Scarano, Francisco; Griffin, Diane E; Holmes, Kathryn V; Murphy, Frederick A; Robinson, Harriet L. Viral pathogenesis. Philadelphia, Lippincott-Raven, 1997. p.267-301, ilus, tab.
Monografia em Inglês | Sec. Est. Saúde SP, SESSP-IALACERVO | ID: biblio-1069972
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