In vivo and in vitro ketamine exposure exhibits a dose-dependent induction of activity-dependent neuroprotective protein in rat neurons.

Anesthetic doses of ketamine induce apoptosis, as well as gene expression of activity-dependent neuroprotective protein (ADNP), a putative homeodomain transcription factor in rat pups (P7). This study investigated if ketamine induced ADNP protein in a dose-dependent manner in vitro and in vivo using primary cultures of cortical neurons and neonatal pups (P7). In vivo immunohistochemistry demonstrated a sub-anesthetic dose of ketamine increased ADNP in the somatosensory cortex (SCC) which was previously identified to be damaged by repeated exposure to anesthetic doses of ketamine. Administration of low-dose ketamine prior to full sedation prevented caspase-3 activation in the hippocampus and SCC. Primary cultures of cortical neurons treated with ketamine (10 µM-10mM) at 3 days-in vitro (3 DIV) displayed a concentration-dependent decrease in expanded growth cones. Furthermore, neuronal production and localization of ADNP varied as a function of both ketamine concentration and length of exposure. Taken together, these data support the model that ADNP induction may be partially responsible for the efficacy of a low-dose ketamine pre-treatment in preventing ketamine-induced neuronal cell death.

Role of large-scale velocity fluctuations in a two-vortex kinematic dynamo.

This paper presents an analysis of the Dudley-James two-vortex flow, which inspired several laboratory-scale liquid-metal experiments, in order to better demonstrate its relation to astrophysical dynamos. A coordinate transformation splits the flow into components that are axisymmetric and nonaxisymmetric relative to the induced magnetic dipole moment. The reformulation gives the flow the same dynamo ingredients as are present in more complicated convection-driven dynamo simulations. These ingredients are currents driven by the mean flow and currents driven by correlations between fluctuations in the flow and fluctuations in the magnetic field. The simple model allows us to isolate the dynamics of the growing eigenvector and trace them back to individual three-wave couplings between the magnetic field and the flow. This simple model demonstrates the necessity of poloidal advection in sustaining the dynamo and points to the effect of large-scale flow fluctuations in exciting a dynamo magnetic field.

The do-not-resuscitate order: associations with advance directives, physician specialty and documentation of discussion 15 years after the Patient Self-Determination Act.

BACKGROUND: Since the passage of the Patient Self-Determination Act, numerous policy mandates and institutional measures have been implemented. It is unknown to what extent those measures have affected end-of-life care, particularly with regard to the do-not-resuscitate (DNR) order. METHODS: Retrospective cohort study to assess associations of the frequency and timing of DNR orders with advance directive status, patient demographics, physician's specialty and extent of documentation of discussion on end-of-life care. RESULTS: DNR orders were more frequent for patients on a medical service than on a surgical service (77.34% vs 64.20%, p = 0.02) and were made earlier in the hospital stay for medicine than for surgical patients (adjusted mean ratio of time from DNR orders to death versus total length of stay 0.30 for internists vs 0.21 for surgeons, p = 0.04). 22.18% of all patients had some form of an advance directive in their chart, yet this variable had no impact on the frequency or timing of DNR ordering. Documentation of DNR discussion was significantly associated with the frequency of DNR orders and the time from DNR to death (2.1 days with no or minimal discussion vs 2.8 days with extensive discussion, p<0.01). CONCLUSIONS: The physician's specialty continues to have a significant impact on the frequency and timing of DNR orders, while advance directive status still has no measurable impact. Additionally, documentation of end-of-life discussions is significantly associated with varying DNR ordering rates and timing.

CT fluoroscopic guidance for percutaneous needle placement into abdominopelvic lesions with difficult access routes.

BACKGROUND: We wished to evaluate the utility of computed tomography fluoroscopy (CTF) for guiding percutaneous abdominopelvic biopsies or fluid aspirations that are considered difficult with conventional computed tomographic (CT) guidance. METHODS: CTF-guided percutaneous biopsy (n = 11) or fluid aspiration (n = 2) was attempted in 13 patients with lesions that were otherwise difficult or potentially unsafe by conventional CT guidance because they were deep to colon, small intestine, or major blood vessels. RESULTS: Using CTF assistance to guide external compression or needle positioning, appropriate needle placement was performed in 11 patients. Biopsy or aspiration was diagnostic in 10 patients. Needle advancement was not attempted in two patients. CONCLUSION: CTF appears to be a valuable tool to dynamically assist percutaneous needle placement into lesions that may be considered difficult with standard CT assistance.

Distortion reduction for fast soft straightening of the colon.

RATIONALE AND OBJECTIVES: The purpose of this study was to suppress the geometric distortion associated with soft straightening of the colon by moderately adjusting curved cross sections, which is equivalent to appropriately modifying the underlying electrical field. MATERIALS AND METHODS: A computational mechanism to reduce the geometric distortion associated with soft straightening was developed. Because the cause of distortion is uneven sampling of the colon wall with curved cross sections, the curved cross sections formed according to a numerically simulated electrical field were redistributed in the distortion reduction process. This mechanism can be directly incorporated into a previously published fast soft-straightening algorithm. Simulations were performed to evaluate the effectiveness of the mechanism, and a phantom colon with digitally implanted, spherical polyps was straightened and rendered to visually display the efficacy of the mechanism. RESULTS: The distortion reduction mechanism was advantageous regarding shape preservation of polyps and alleviated distortion substantially. The mechanism occupied only a small portion of the total processing time. CONCLUSION: The distortion reduction mechanism can effectively reduce the geometric distortion associated with soft straightening at little computational cost and has potential for use with computed tomographic colonography.

Fast algorithm for soft straightening of the colon.

RATIONALE AND OBJECTIVES: In this study, the authors developed a fast algorithm for soft straightening of the colon with computed tomographic data that greatly accelerates the unraveling process based on the interpolation of representative electric force lines. MATERIALS AND METHODS: Each curved cross section of the colon is defined by electric force lines of a common origin on an electrically charged central path and is constructed by interpolating most of these force lines from a limited number of representative force lines that are traced directly. Both a synthetic colon phantom and a colon in a living patient were used to demonstrate the feasibility of the fast interpolation algorithm compared with direct implementation for soft straightening of the colon. RESULTS: The interpolation-based soft-straightening algorithm ran approximately 40 times faster than the direct implementation of the electric field-based soft-straightening algorithm. CONCLUSION: The fast algorithm for soft straightening of the colon has potential for use in computed tomographic colonography.

CT fluoroscopy: technique and utility in guiding biopsies of transiently enhancing hepatic masses.

We evaluated a new percutaneous biopsy technique for rapid needle localization and biopsy of transiently enhancing focal hepatic masses. Three biopsies in three patients were completed on lesions 18-22 mm in diameter by using computed tomographic fluoroscopy during intravenous contrast enhancement of the liver. All three biopsies were diagnostic.

Straightening the colon with curved cross sections: an approach to CT colonography.

RATIONALE AND OBJECTIVES: The purpose of this study was to straighten digitally and consistently the colon with curved cross sections and to compare the results with planar cross-section-based processing for computed tomographic (CT) colonography. MATERIALS AND METHODS: In electric field-based straightening, curved cross sections are formed along electric force lines because of electric charges digitally distributed along the colon central path. Four straightening experiments were conducted on CT scans of a colonoscopy phantom. Representative images were studied for polyp detectability and feature distortion. Two further trials involved patient data to demonstrate the clinical feasibility of this method. RESULTS: In colon straightening with planar sections, a polyp was counted multiple times in both phantom and patient studies where the polyps were in central path turns with substantial curvature. Furthermore, opposite the central path turns, the colon walls were undersampled with planar sections. Straightening with curved sections produced consistent mappings. Image distortion was present in straightening with curved sections, but the conspicuity of polyps was maintained. In the soft-straightening process, trilinear interpolation greatly suppressed the surface- or volume-rendering noise associated with nearest neighbor interpolation. CONCLUSION: Straightening with curved sections outperforms straightening with planar sections in terms of polyp detectability. This approach eliminates the navigation difficulties of current CT colonography and may have clinical use.

Optimizing contrast enhancement during helical CT of the liver: a comparison of two bolus tracking techniques.

OBJECTIVE: The purpose of this study was to evaluate a recently developed hardware and software system for CT scanning that generates images in real time and switches to helical CT scanning by either a visual cue or a region of interest (ROI) amplitude threshold. SUBJECTS AND METHODS: We randomly and prospectively divided 120 abdominal CT examinations into three groups. Two groups received 75 ml of contrast agent at 1.5 ml/sec. Helical CT scanning began after visualization of the contrast bolus arrival in the hepatic veins (visual cue triggering) (39 patients) or after reaching an ROI threshold (automated ROI threshold triggering) (39 patients). A third group served as a control group (42 patients) and received 150 ml of contrast agent at 1 ml/sec. Quality of hepatic enhancement was assessed objectively and subjectively. Comparisons were made after stratifying each group into three weight classes. RESULTS: Errors occurred in 12 (31%) of 39 examinations in the group with automated ROI threshold triggering. In that group, we found a significantly (p < .04) lower mean hepatic enhancement in two of three weight categories, and a significantly (p < .04) lower mean subjective scan quality in one of three weight categories, than we found in the group with visual cue triggering. CONCLUSION: Optimizing portal venous phase helical CT of the liver after a low-volume bolus of contrast agent and an injection rate of 1.5 ml/sec is best achieved by initiating helical CT scanning after visualizing the contrast bolus arrival within the liver rather than after reaching a preset attenuation threshold.

GI tract unraveling with curved cross sections.

Gastrointestinal (GI) tract examination with spiral/helical computed tomography (CT) is currently performed by slice-based inspection of axial images. CT colography is a recent advance which allows an intraluminal visualization of the colon, similar to endoscopy. Various rendering algorithms have been developed with promising results, however navigation through the complex, tortuous anatomy of the colon can be time consuming in practice. In this paper, we propose an electrical-field-based method to unravel the convoluted colon, that is, to digitally straighten it with curved cross sections and flatten it over a plane. In our method, electrical charges are simulated along the central colon path. Curved cross sections are defined by the electrical force lines, and lead to consistent unraveling. It is demonstrated with image volumes of two patients that this technique produces a global planar view of complicated colon features with a potential for detection of polyps.

Teaching physical examination of the adult liver with use of real-time sonography.

RATIONALE AND OBJECTIVES: The authors developed and evaluated an approach to teaching examination of the liver that incorporates real-time ultrasound (US) imaging as immediate feedback to improve diagnostic accuracy. MATERIALS AND METHODS: Second-year medical students participating in a course in physical examination were assigned to receive instruction in measuring liver span without (group 1) or with (group 2) real-time sonography. Students from each group attended a practice session given by one of two physicians who were board certified in radiology and internal medicine with special expertise in US. During the practice session for group 2, students were shown the boundaries of the liver of the practice patient with real-time US. Both groups of students then made three measurements each of the liver span of a healthy practice patient and a single healthy test patient without US. The vertical liver span reference standard was determined by one of the authors. RESULTS: Students in group 2 showed greater accuracy in measuring liver span during both the practice and the test sessions than did students in group 1. The differences were significant for the third practice measurement and all three test measurements (P < .05). CONCLUSION: The use of real-time US as an aid in teaching physical examination improves students' accuracy in measuring liver size.

Contractions move contents by changing the configuration of the isolated cat stomach.

To understand how contractions move gastric contents, we measured, in isolated cat stomachs, the effects of contractions on gastric length, diameters, pressures, and emptying. Movements of the stomach and of gastric contents were monitored by video camera and ultrasound and were related to mechanical events. Based on pressures, we defined the following four phases of contractions: 1) Po, a steady pressure associated with tonic contraction of proximal stomach; 2) P', a pressure wave during which the contraction indents the gastric body; 3) a pressure nadir while the contraction lifts the gastric sinus toward the incisura; and 4) a second pressure wave, P", as the contraction advances through the antrum. In open preparations, liquid output and shortening of the greater curvature are large during Po, stop during P', and resume with P". Contractions generate higher pressures when gastric volume is held steady. Contractions increase wall thickness and decrease gastric diameters at sites they involve and have opposite effects at remote sites. Contractions move the incisura and hence redraw the borders between gastric segments and shift volumes back and forth within the gastric lumen. Contractions furthermore stir up, compress, and disperse particulate beans without moving them to the pylorus. We conclude that gastric contractions 1) reverse changes in gastric length that occur during gastric filling, 2) move gastric contents directly through local contact and indirectly by changing the configuration of the stomach, and 3) interact with structures such as the incisura in retaining and breaking up solid gastric contents.

Normal lower limb venous Doppler flow phasicity: is it cardiac or respiratory?

OBJECTIVE: The purposes of this study were to determine the origin and nature of normal lower limb venous Doppler flow phasicity and to assess normal and respiratory variations. SUBJECTS AND METHODS: The common femoral veins of 12 healthy volunteers (three men and nine women; age range, 21-50 years; mean, 29 years) were evaluated by detailed spectral Doppler examinations with simultaneous ECG and respirometric tracings. The examinations were performed using a 5- or 7-MHz linear-array transducer with breath held in mid respiration, at the end of deep expiration, at the end of deep inspiration, during Valsalva's maneuver, and during quiet and deep breathing. The tracing obtained during breath-hold in mid respiration was considered the baseline. Tracings obtained during the other respiratory phases were analyzed for changes from the baseline. Doppler tracings were analyzed for phasicity, waveform frequency, components, velocities, velocity ratios, and presence of retrograde flow, all in correlation with simultaneous ECG and respirometric tracings. Tracings were analyzed independently by two observers to assess interobserver variability. RESULTS: With breath-hold in mid respiration, the common femoral vein Doppler tracings consisted of multiphasic waveforms that had a frequency similar to that of the heart rate. Each waveform consisted of systolic, v, diastolic, and a waves. The systolic wave occurred 0.4 sec later than the QRS complex of the ECG and was always antegrade. The v wave was always retrograde without flow reversal. The diastolic wave was always antegrade. The a wave was always retrograde but showed flow reversal in nine of 12 subjects. The systolic:diastolic velocity ratio ranged from 0.9 to 1.5 (mean, 1.1). The minimum:maximum velocity ratio ranged from -0.4 to 0.2 (mean, -0.15). With breath-hold at the end of expiration, the waveforms became slightly damped, becoming biphasic in five subjects and remaining multiphasic in seven. With breath-hold at the end of inspiration, the waveforms became nonphasic or biphasic in nine and decreased in velocity in 12. With Valsalva's maneuver, flow stopped. With normal respiration, cardiac waveforms were modulated by higher amplitude and less frequent biphasic respiratory waves. The plasticity was equal in two, dominantly cardiac in six, and dominantly respiratory in four. Flow velocity increased with expiration and decreased with inspiration. With deep breathing, the respiratory waves further increased, while the cardiac ones decreased in amplitude. The latter continued to modulate the respiratory phasicity in 10 subjects. CONCLUSION: During quiet respiration, lower limb venous Doppler tracings consisted of both cardiac and respiratory waveforms. Although respiratory waveforms disappeared when patients held their breath, Doppler tracings continued to be multiphasic and cardiac. Therefore, cardiac phasicity in lower limb venous Doppler tracings does not necessarily indicate cardiac disease. Other respiratory phases can modulate this basic cardiac pattern. Decrease in or loss of phasicity in these waveforms does not always mean proximal obstruction, because it can be caused by respiratory factors. Finally, the presence of minimal cyclic retrograde flow that is 5 cm/sec or less does not necessarily indicate cardiac disease.

Suicides associated with the Jacques Cartier Bridge, Montreal, Quebec 1988-1993: descriptive analysis and intervention proposal.

Falls from heights represent an uncommon means of suicide. Regional variations are attributable to the presence of particular sites which attract suicidal individuals. The Jacques Cartier Bridge in Montreal is one such site, though less well known than North American sites such as the Golden Gate Bridge or Niagara Falls. According to Coroner's records, 54 suicides were associated with the bridge for the period 1988 to 1993. All but one of the suicides were the result of jumps from the bridge. The median age of victims was 30 years, and 46 of the victims were male. Bridge-specific verbalization of suicidal intent and prior history of medically diagnosed psychiatric disorders are frequently noted. Based on a review of the effectiveness of preventive measures, we propose limiting access to jumping by means of a fence along the bridge railing.

Volume shifts, unfolding and rolling of haustra in the isolated guinea pig caecum.

The large intestine extracts water from chyme and compacts chyme into faecal conglomerates; it is unclear what role the special pockets known as colonic haustra have in these events. Here we monitored the movements of haustra in isolated preparations of guinea pig caecum using videocamera and ultrasound and related them to contractions of muscle flaps and movements of glass beads in haustral pockets. We found that in partially filled caecal loops localized contractions of taeniae shift volume back and forth between adjacent haustra; volume unfolds haustral walls in a characteristic sweep with sequential intrahaustral folds popping out; cyclic contractions and relaxations of the fold then produce the caterpillar-like movement known as haustral rolling; ultrasound showed that haustral rolling made the haustral flow channel narrower and longer as haustral folds increase their height from 7.5 +/- 1.5 mm to 16 +/- 4 mm and their distance from 4.1 +/- 0.2 mm to 7.9 +/- 0.3 mm; luminal contents were alternatively shaken off the haustral wall, whirled around the lumen or left to settle. We also suspended the row of haustra between two taeniae inside a frame and attached flaps of taeniae and haustral folds to strain gauges to record their mechanical activity; both taeniae and haustral folds produced an undulating baseline tension; during rolling, folds produced phasic contractions at 17 +/- 2 cycles min-1 which propagated distally across haustral septa; rolling constantly shuffled around glass beads placed inside the haustra. When we stimulated the intramural nerves to the caecum through bipolar electrodes, all contractile activity was temporarily inhibited and haustral septa flattened; a rebound contraction then propagated aborally from the caecal pole and swept the glass beads ahead of it. Thus, tonic contractions of taeniae shift caecal contents back and forth across haustral septa; expansion of haustra triggers haustral rolling which shuffles contents; both these movements produce local flow within and between haustra which might enhance the separation of solid and liquid colonic contents.

Paraovarian and paratubal cysts: preoperative diagnosis using transabdominal and transvaginal sonography.

OBJECTIVE: Paraovarian and paratubal cysts constitute about 10% of adnexal masses. Our purpose was to determine whether pathologically proven examples of these lesions were correctly identified during preoperative transabdominal and transvaginal sonographic evaluations. METHODS: Between 1990 and 1994, 15 women with 10 paraovarian cysts and 5 paratubal cysts undewent preoperative sonography. The sonograms were performed using transabdominal and transvaginal probes. Mean interval before surgery was 22 days (range: 1-96 days). The sonographic interpretation at the time of the examination was correlated with the pathological findings. RESULTS: In only 1 of 15 patients was a paraovarian or paratubal cyst suggested before surgery. Paraovarian cysts were misdiagnosed as ovarian cysts. Because of the microscopic size of paratubal cysts in our series, none was diagnosed before surgery and these were usually an incidental finding in patients with ovarian masses. CONCLUSIONS: In our experience, paraovarian and paratubal cysts are difficult to diagnose before surgery with the use of transabdominal and transvaginal sonography. We speculate that when there is close proximity of a paraovarian cyst to the ovary, an ovarian cystic mass cannot reliably be differentiated from a paraovarian cyst.

Duplex Doppler sonography of changes in portal vein flow in healthy term newborn infants after feeding.

To establish reference values for changes in portal venous diameter, angle- corrected maximal flow velocity, and flow in healthy term newborn infants after formula feeding, we studied 20 subjects using duplex Doppler sonography. After feeding, portal venous diameter increased from 3.6 +/- 0.1 (mean +/- standard error of the mean) to 3.9 +/- 0.1 mm at 15 min and decreased to 3.8 +/- 0.1 at 60 min. Maximal flow velocity increased from 24.1 +/- 1.3 cm/s to a maximum of 35.9 +/- 2.4 cm/s at 15 min and decreased to 28.8 +/- 1.5 cm/s at 60 min. Flow increased from 85.0 +/- 7.5 ml/min to a maximum of 153.6 +/- 14.9 ml/min at 15 min with decrease similar to the maximal flow velocity curve. We conclude that formula feeding produces peak portal blood flows of nearly twice the fasting values at 15 min after feeding and returns almost to fasting value by 60 min.

Haustral septations increase axial and radial distribution of luminal contents in glass models of the colon.

We used two glass models of the colon to test the hypothesis that luminal septations increase efficiency of flow. Each was a straight glass tube 4 cm in diameter and filled with water. One had four septations narrowing the lumen to 2 cm; the other had no septations. For each run, liquid dye and one solid test particle were placed at one end of the model. A single pressure pulse was applied to the dye and particle. The distance each traveled was compared using analysis of variance. The nonseptated model had poor mixing of dye, and the particle moved only short distances. In the septated model, there was thorough mixing of dye and the particle moved rapidly along what appeared as a central core of high-velocity liquid flow from one septal opening to another. Compared with the nonseptated model, this produced statistically significant increases in the distances traveled by means of 32 (dye) and 90% (particle). Our studies suggest that septations in a tubular organ facilitate intraluminal mixing and flow of liquids and solids.

Laparoscopic partial nephrectomy: initial experience and comparison to the open surgical approach.

During an 18-month period, 6 laparoscopic partial nephrectomies were attempted, 4 of which were successful. The surgical technique was modified and improved between cases aided by new laparoscopic instrumentation, such as the argon beam coagulator and the 7.5 MHz. ultrasonic sector scanning system. In a retrospective comparison between laparoscopic and open partial nephrectomy, estimated blood loss was 525 ml. for the former versus 708 ml. for the latter procedure. However, operating time was more than 2 hours longer with the laparoscopic approach. The major advantages of the laparoscopic procedure appear to be a more rapid return to full diet, less postoperative pain and less requirement for parenteral narcotics. Despite the small size of this series and limited followup data, convalescence may be shortened by 4 weeks after laparoscopic partial nephrectomy. Patients with benign diseases of the kidney, especially with a duplicated collecting system, who require partial nephrectomy may be considered candidates for the laparoscopic approach. The advantages to the patient, however, may be offset by the technical demands on the surgeon.