Social housing influences the composition of volatile compounds in the preputial glands of male rats.

In rodents the preputial glands are one of the major sources of pheromones. These volatile chemosignaling compounds are known to elicit specific behavioral and physiological effects in their conspecifics. While social stress can alter both the behavior and hormonal status of rodents, little is known about its influence on the volatile constituents of the preputial glands. We have examined the composition of volatile compounds in the preputial glands of gonadally intact male rats housed for 70 days in either unisex triads (three/cage) or singly. The rank status of triad-housed rats was based on quantitative behavioral assessments taken during the initial 30 min of triad housing. Dominant rats had heavier preputial glands compared to subdominant and subordinate rats. Capillary gas chromatography-mass spectrometry identified 56 volatile preputial compounds, of these 17 did not differ between groups while 26 compounds were significantly higher in the single-housed compared to the triad-housed rats. Six additional volatile compounds were higher in the dominant compared to the other 3 groups, while another six compounds were higher in both the dominant and single-housed rats compared to the subdominant and subordinate rats. It can be concluded that both housing condition and social rank status have significant but different effects on the composition of volatile compounds found in preputial glands of male rats. The physiological and behavioral significance of these changes in preputial gland volatile compound composition in rats remain to be investigated.

Putative chemosignals of the ferret (Mustela furo) associated with individual and gender recognition.

Quantitative stir bar sorptive extraction methods, both in the aqueous and headspace modes, followed by thermal desorption gas chromatography-mass spectrometry were used to investigate individual variations in the volatile components of male and female ferret (Mustela furo) urine. The urinary profiles were further compared with volatile profiles of anal gland secretions of breeding male and female ferrets. Thirty volatile compounds were quantified in male and female urine. Among them, 2-methylquinoline was unique to male urine. Four ketones (4-heptanone, 2-heptanone, o-aminoacetophenone, and a dimethoxyacetophenone) and several nitrogen compounds (e.g., 2,5-dimethylpyrazine, quinoline, 4-methylquinazoline) and low levels of three unidentified nonsulfur compounds were significantly more abundant in males than in females. Quantitative comparison of 30 volatile urinary compounds showed several statistically significant differences between the sexes and individuals of the same sex. These findings suggest that ferrets may use urine marking for sex and individual recognitions. Ten of the 26 compounds identified in anal gland secretions from females and males were also found in urine. However, most of the major compounds (thietanes, dithiolanes, and indole) in anal glands were not present in urine. This suggests that urine may convey specific signals that differ from those of anal glands. Additionally, 10 volatiles (two aldehydes, five ketones, benzothiazole, 2-methylquinoline, and 4-methylquinazoline), not previously identified, were found in ferret anal gland secretions. Among the new compounds, o-aminoacetophenone was found only in males, while only traces of this compound were found in females. Similar results were previously obtained in anal glands of three other Mustela species. These findings provide new information about the constituents of urine and volatile components of anal gland secretions in ferrets.

College students' attitudes about AIDS: 1986 to 2000.

College students' attitudes about AIDS and people with AIDS (PWAs) were measured over a 15-year period. The AIDS Attitude Scale, designed by Shrum, Turner, and Bruce (1989; AIDS Education and Prevention, 1, 222-230), was administered to introductory psychology students (n = 1,571) at one midsized southeastern university, thus allowing direct comparison of attitudes over time. Overall tolerance about AIDS and PWAs has increased from 1986 to 2000 and robust gender differences in attitudes have been apparent over time, with females expressing more tolerant attitudes. Concerns about contagion from casual contact are diminishing as well and perceived knowledge about AIDS has increased over time. Perceptions about personal susceptibility to HIV remain low and show little relationship to attitudes about AIDS and PWAs. These data may be used to help refine HIV prevention programs for college students and provide an example of a useful approach to monitor changes in attitudes over time.

A scale to measure attitudes about HIV-antibody testing: development and psychometric validation.

Testing for antibodies to HIV is a vital component of many AIDS prevention and intervention programs. To increase the use of HIV antibody testing, it is important to understand more about peoples' attitudes about testing so that intervention programs can focus on the specific concerns of the target population. A scale to measure attitudes about HIV antibody testing (HIV-Antibody Testing Attitude Scale, HTAS) was developed and tested for reliability and validity, using data from two samples of heterosexual college students. The HTAS is a 32-item Likert-type scale, with high internal consistency (Cronbach's alpha = .88). Scores on the HTAS correlate with self-reported testing and intention to be tested for HIV, but not with other types of self-protective behavior (e.g., condom use), indicating evidence for its discriminant validity. Preliminary factor analysis of the responses to the HTAS shows four factors: concerns about friend's responses, concerns about family's responses, beliefs about public opinion of testing, and concerns about confidentiality of tests. The HTAS is easy to administer and offers a tool to use for both designing and evaluating HIV intervention and prevention programs that emphasize HIV antibody testing.

Assessing the construct validity of the AIDS Attitude Scale.

To assess the construct validity of the AIDS Attitude Scale (AAS) (Shrum, Turner, & Bruce, 1989), we administered it and related attitude scales to introductory psychology students (n = 279) and gay/lesbian support group members (n = 38). A subset of the students (n = 105) participated in a concurrent validity study; students donating items to an AIDS food pantry scored more tolerantly on the AAS than other students. AAS scores also differentiated subjects expected to have more tolerant attitudes toward people living with HIV and AIDS from other respondents, indicating known groups validity. As expected, AAS scores were positively correlated with attitudes about homosexuals and negatively correlated with authoritarian beliefs. However, AAS scores were not related to death anxiety. Attitudes about AIDS were distinguishable from related constructs using factor analysis. Together, these data provide evidence for the construct validity of the AAS. The need for valid assessment tools and uses of the AAS are discussed.

Development of an instrument to measure attitudes toward acquired immune deficiency syndrome.

The AIDS Attitude Scale (AAS) was developed to measure attitudes toward acquired immune deficiency syndrome (AIDS) among college students. In phase one, 67 items, determined by an expert panel to have content validity, were randomly ordered and administered to 164 student volunteers in a large southern university. Fifty-four individual items that correlated significantly (p less than .001) with the total attitude scores were retained for the final version of the scale. In phase two, the revised scale was administered to 135 student volunteers to determine its internal reliability (alpha = .96). Females and older students (21 and over) were significantly more tolerant about AIDS than males and younger students. Preliminary factor analysis revealed three factors explaining approximately 45% of the variance in response patterns; they related to proximity with people with AIDS, moral issues, and social welfare issues. Uses of the instrument in attitudinal studies, program planning, and evaluation are discussed.

Sexual behavior of group-housed stumptail macaques (Macaca arctoides): temporal, demographic and sociosexual relationships.

A detailed quantitative analysis of sexual behavior in a social group of captive stumptail macaques was conducted. The distribution of copulations both over days and among animals, and the relationships between copulation and various sociosexual patterns were investigated. Copulations occurred erratically over days and were preferentially directed to a small minority of females. Most patterns of sociosexual behavior were at their highest rates during actual copulatory episodes, others occurred independent of ejaculation. It is suggested that the relationships between variables such as social rank, age and parity and sociosexual patterns are quite flexible and probably vary with the species, testing conditions and demographic make-up of the group.