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Anal Bioanal Chem ; 407(5): 1285-94, 2015 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-25524230

RESUMO

Detection of protein biomarkers is of major interest in proteomics. This work reports the analysis of protein biomarkers directly from a biological fluid, human saliva, by surface plasmon resonance imaging coupled to mass spectrometry (SPRi-MS), using a functionalized biochip in an array format enabling multiplex SPR-MS analysis. The SPR biochip presented a gold surface functionalized by a self-assembled monolayer of short poly(ethylene oxide) chains carrying an N-hydroxysuccinimide end-group for the immobilization of antibodies. The experiments were accomplished without any sample pre-purification or spiking with the targeted biomarkers. SPRi monitoring of the interactions, immune capture from the biochip surface, and finally on-chip matrix-assisted laser desorption/ionization-MS structural identification of two protein biomarkers, salivary α-amylase and lysozyme, were successively achieved directly from saliva at the femtomole level. For lysozyme, the on-chip MS identification was completed by a proteomic analysis based on an on-chip proteolysis procedure and a peptide mass fingerprint.


Assuntos
Biomarcadores/química , Saliva/química , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz/métodos , Ressonância de Plasmônio de Superfície/métodos , Humanos , Muramidase/química , Mapeamento de Peptídeos , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz/instrumentação , Ressonância de Plasmônio de Superfície/instrumentação , alfa-Amilases/química
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