RESUMO
The mechanisms of therapeutic resistance of human glioblastoma multiforme are analyzed. The authors make an attempt at systematization and scientific theoretical validation of new approaches to creation of biomedical cellular preparations, based on the oncoproteomic technologies, for personified therapy of the glial tumors. A new approach to the treatment of glioblastoma multiforme with due consideration for the molecular biological characteristics of the tumor stem cells is suggested. It is shown that the tumor stem cell proteome can be regarded as the main target for cell therapy.
Assuntos
Glioblastoma/metabolismo , Glioblastoma/terapia , Genômica , Humanos , Células-Tronco Neoplásicas/metabolismo , Proteômica , Transcriptoma/genéticaRESUMO
The effects of neural progenitor and hemopoietic stem cells on C6 glioma cells were studied in in vivo and in vitro experiments. Considerable inhibition of proliferation during co-culturing of glioma cells with neural progenitor cells was revealed by quantitative MTT test and bromodeoxyuridine incorporation test. Labeled neural progenitor and hemopoietic stem cells implanted into the focus of experimental cerebral glioma C6 survive in the brain of experimental animals for at least 7 days, migrate with glioma cells, and accumulate in the peritumoral space. Under these conditions, neural progenitor cells differentiate with the formation of long processes. Morphometric analysis of glioma cells showed that implantation of neural progenitor and hemopoietic stem cells is accompanied by considerable inhibition of the growth of experimental glioma C6 in comparison with the control. The mechanisms of tumor-suppressive effects of neural and hemopoietic stem cells require further investigation.
Assuntos
Neoplasias Encefálicas/terapia , Glioma/terapia , Células-Tronco Hematopoéticas/citologia , Células-Tronco Neurais/citologia , Animais , Células da Medula Óssea , Neoplasias Encefálicas/patologia , Proliferação de Células , Sobrevivência Celular , Técnicas de Cocultura , Feminino , Glioma/patologia , Transplante de Células-Tronco Hematopoéticas , Humanos , Injeções Intralesionais , Microscopia de Fluorescência , Cavidade Nasal/citologia , Transplante de Neoplasias , Células-Tronco Neurais/transplante , Mucosa Olfatória/citologia , Ratos , Ratos Wistar , Fase S , Transplante HeterólogoRESUMO
The results presented in our previous report (Morfologiya, 127, No. 2 (2005)) provided evidence that consolidation of the spinal cord (SC) after thoracic segmentectomy in rats occurs as a result of the formation of a connective tissue scar, which is quicker when the defect is filled with collagen gel. The present report describes analysis of semithin sections and transmission electron microscopy studies demonstrating that regenerating nerve conductors traverse connective tissue in structures whose organization is identical to that of peripheral nerves. In the zone of SC rarefaction caudal to the trauma site, myelination of growing axons is mediated by glial cells without the formation of nerve trunks. Large numbers of fine regenerating conductors were seen at the sites of degenerated myelin fibers in the fasciculi of the white matter in the lumbar segments of the SC.
