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2.
J Clin Invest ; 124(5): 1887-8, 2014 May.
Artigo em Inglês | MEDLINE | ID: mdl-24789881

RESUMO

The inactivation of NO by advanced glycation endproducts (AGEs), which accumulate on tissue proteins as a function of age and hyperglycemia, focused attention on the role of these ubiquitous posttranslational modifications in acquired impairments of vascular reactivity and other signaling processes. This observation occurred during a watershed period of basic and translational research in glycation that encompassed new pathologic phenomena and novel intervention strategies. How has the AGE paradigm for the tissue complications of aging and diabetes fared since the identification of the link between these glycation products and NO inactivation, and what lessons may be offered for future investigations?


Assuntos
Envelhecimento/metabolismo , Envelhecimento/patologia , Diabetes Mellitus , Produtos Finais de Glicação Avançada/metabolismo , Óxido Nítrico/metabolismo , Diabetes Mellitus/metabolismo , Diabetes Mellitus/patologia , Diabetes Mellitus/terapia , Humanos , Hiperglicemia/metabolismo , Hiperglicemia/patologia , Hiperglicemia/terapia
3.
Cell ; 156(3): 456-68, 2014 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-24485454

RESUMO

The phagocytes of the innate immune system, macrophages and neutrophils, contribute to antibacterial defense, but their functional specialization and cooperation is unclear. Here, we report that three distinct phagocyte subsets play highly coordinated roles in bacterial urinary tract infection. Ly6C(-) macrophages acted as tissue-resident sentinels that attracted circulating neutrophils and Ly6C(+) macrophages. Such Ly6C(+) macrophages played a previously undescribed helper role: once recruited to the site of infection, they produced the cytokine TNF, which caused Ly6C(-) macrophages to secrete CXCL2. This chemokine activated matrix metalloproteinase-9 in neutrophils, allowing their entry into the uroepithelium to combat the bacteria. In summary, the sentinel macrophages elicit the powerful antibacterial functions of neutrophils only after confirmation by the helper macrophages, reminiscent of the licensing role of helper T cells in antiviral adaptive immunity. These findings identify helper macrophages and TNF as critical regulators in innate immunity against bacterial infections in epithelia.


Assuntos
Infecções Bacterianas/imunologia , Macrófagos/imunologia , Neutrófilos/imunologia , Infecções Urinárias/imunologia , Animais , Antígenos Ly/metabolismo , Quimiocina CXCL2/imunologia , Feminino , Doenças do Sistema Imunitário , Cinética , Transtornos Leucocíticos , Macrófagos/citologia , Metaloproteinase 9 da Matriz/metabolismo , Camundongos , Neutrófilos/citologia , Organismos Livres de Patógenos Específicos , Fator de Necrose Tumoral alfa/imunologia
5.
J Infect Dis ; 203(2): 211-9, 2011 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-21288821

RESUMO

BACKGROUND: The mechanisms of severe malarial anemia and cerebral malaria, which are extreme manifestations of Plasmodium falciparum malaria, are not fully understood. METHODS: Children aged <6 years from southern Zambia presenting to the hospital with severe malarial anemia (n = 72), cerebral malaria (n = 28), or uncomplicated malaria (n = 66) were studied prospectively. Children with overlapping severe anemia and cerebral malaria were excluded. RESULTS: Low interleukin 10 concentrations had the strongest association with severe anemia (standard ß = .61; P < .001) followed by high tumor necrosis factor α and sFas concentrations, low weight-for-age z scores, presence of stool parasites, and splenomegaly (standard ß = .15-.25; P ≤ .031); most of these factors were also associated with lower reticulocytes. Greater parasitemia was associated with higher interleukin 10 and tumor necrosis factor α concentrations, whereas sulfadoxizole/pyrimethamine therapy and lower weight-for-age z scores were associated with lower interleukin 10 levels. Thrombocytopenia and elevated tissue plasminogen activator inhibitor 1 levels had the strongest associations with cerebral malaria (standard ß = .37 or .36; P < .0001), followed by exposure to traditional herbal medicine and hemoglobinuria (standard ß = .21-.31; P ≤ .006). CONCLUSIONS: Predictors of severe malarial anemia (altered immune responses, poor nutrition, intestinal parasites, and impaired erythropoiesis) differed from those of cerebral malaria (thrombocytopenia, herbal medicine, and intravascular hemolysis). Improved preventive and therapeutic measures may need to consider these differences.


Assuntos
Febre Hemoglobinúrica/imunologia , Febre Hemoglobinúrica/patologia , Malária Cerebral/imunologia , Malária Cerebral/patologia , Malária Falciparum/imunologia , Malária Falciparum/patologia , Pré-Escolar , Feminino , Humanos , Lactente , Malária Falciparum/complicações , Masculino , Plasmodium falciparum/imunologia , Plasmodium falciparum/patogenicidade , Fatores de Risco , Zâmbia
6.
Circ Res ; 105(1): 99-107, 2009 Jul 02.
Artigo em Inglês | MEDLINE | ID: mdl-19478200

RESUMO

Chronic inflammation in white adipose tissue (WAT) is positively associated with obesity, insulin resistance (IR) and the development of type 2 diabetes. The proinflammatory cytokine MIF (macrophage migration inhibitory factor) is an essential, upstream component of the inflammatory cascade. This study examines whether MIF is required for the development of obesity, IR, glucose intolerance, and atherosclerosis in the LDL receptor-deficient (Ldlr(-/-)) mouse model of disease. Ldlr(-/-) mice develop IR and glucose intolerance within 15 weeks, whereas Mif(-/-)Ldlr(-/-) littermates are protected. MIF deficiency does not affect obesity and lipid risk factors but specifically reduces inflammation in WAT and liver, as reflected by lower plasma serum amyloid A and fibrinogen levels at baseline and under inflammatory conditions. Conversely, MIF stimulates the in vivo expression of human C-reactive protein, an inflammation marker and risk factor of IR and cardiovascular disease. In WAT, MIF deficiency reduces nuclear c-Jun levels and improves insulin sensitivity; MIF deficiency also reduces macrophage accumulation in WAT and blunts the expression of two proteins that regulate macrophage infiltration (intercellular adhesion molecule-1, CD44). Mechanistic parallels to WAT were observed in aorta, where the absence of MIF reduces monocyte adhesion, macrophage lesion content, and atherosclerotic lesion size. These data highlight the physiological importance of chronic inflammation in development of IR and atherosclerosis and suggest that MIF is a potential therapeutic target for reducing the inflammatory component of metabolic and cardiovascular disorders.


Assuntos
Tecido Adiposo Branco/patologia , Inflamação/etiologia , Resistência à Insulina , Fatores Inibidores da Migração de Macrófagos/deficiência , Animais , Aterosclerose/etiologia , Biomarcadores/sangue , Doença Crônica , Intolerância à Glucose/etiologia , Humanos , Fígado/patologia , Camundongos , Camundongos Knockout , Camundongos Transgênicos , Receptores de LDL/deficiência
7.
J Leukoc Biol ; 75(3): 398-9, 2004 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-14634053

RESUMO

This series of overviews was contributed by participants of a Keystone Symposium, which was convened in early 2003 to address the topic of septic shock in an interdisciplinary manner. This occasion reflects a resurgence of interest in septic shock as a disease that may soon be approachable by new therapeutic interventions.


Assuntos
Choque Séptico/etiologia , Adjuvantes Imunológicos , Animais , Humanos , Inflamação/imunologia , Choque Séptico/tratamento farmacológico , Choque Séptico/patologia
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