Assuntos
Anticorpos Heterófilos/sangue , Ciclofosfamida/farmacologia , Citotoxicidade Imunológica , Fígado/imunologia , Transplante Heterólogo/imunologia , Animais , Anticorpos Heterófilos/biossíntese , Formação de Anticorpos/efeitos dos fármacos , Ensaio de Imunoadsorção Enzimática , Feminino , Cobaias , Imunoglobulina G/sangue , Imunoglobulina M/sangue , Linfócitos/efeitos dos fármacos , Linfócitos/imunologia , Masculino , Ratos , Ratos Endogâmicos Lew , Fatores de TempoRESUMO
Thirty nine phenotypes of human leucocyte antigens (HLA)-A-B-DR and DQ were obtained from 99 asbestos workers (one woman and 98 men). Presence or absence of antinuclear antibodies and rheumatoid factor was determined in 91 of them. Workers were divided into five groups: asbestos workers with no apparent disease (AW; n = 17), diffuse benign pleural disease (PD; n = 31), asbestosis (AS; n = 24), asbestosis with lung cancer (AS-CA; n = 14), and mesothelioma (M; n = 13). Compared with AW, several trends of differences of HLA antigen prevalence were found in patients with asbestos related disease, but these did not achieve statistical significance when p was corrected (pcorr) by number of analyses undertaken. Analysis of the results obtained in previous studies together with the results of this study showed that compared with AW, AS patients had decreased prevalence of HLA-DR5 (pcorr < 0.02). Reasons for the differences in results of previous studies and statistical methods commonly used to compare prevalences of HLA antigen are discussed.
Assuntos
Asbestose/imunologia , Antígenos de Histocompatibilidade Classe II/análise , Antígenos de Histocompatibilidade Classe I/análise , Adulto , Idoso , Anticorpos Antinucleares/análise , Feminino , Antígenos de Histocompatibilidade Classe I/genética , Antígenos de Histocompatibilidade Classe II/genética , Humanos , Masculino , Pessoa de Meia-Idade , Fator Reumatoide/análiseRESUMO
A kinetic assay for alanine transferase (ALT) using microtitre plates and commercially available reagents was developed to retain the advantages conferred by multichannel and semiautomated equipment. The time taken to assay 92 samples was less than 10 minutes, and costs of apparatus and reagents compared favourably with most current methods. Although reactions became non-linear above 120 IU/l-1, higher concentrations were "flagged" and accuracy was satisfactory below this point.
