Potential methylation-regulated genes and pathways in hepatocellular neoplasm, not otherwise specified.

Background and Aims: The molecular basis of hepatocellular neoplasm, not otherwise specified (HCN-NOS) is unknown. We aimed to identify gene expression patterns, potential methylation-regulated genes and pathways that characterize the tumor, and its possible relationship to hepatoblastoma and hepatocellular carcinoma (HCC). Approach & Results: Parallel genome-wide profiling of gene expression (RNAseq) and DNA methylation (EPIC850) was performed on 4 pairs of pre-treatment HCN-NOS tumors and adjacent non-tumor controls. 2530 significantly differentially expressed genes (DEGs) were identified between tumors and controls. Many of these DEGs were associated with hepatoblastoma and/or HCC. Analysis Match in Ingenuity Pathway Analysis determined that the gene expression profile of HCN-NOS was unique but significantly similar to that of both hepatoblastoma and HCC. A total of 27,195 CpG sites (CpGs) were significantly differentially methylated (DM) between tumors and controls, with a global hypomethylation pattern and predominant CpG island hypermethylation in promotor regions. Aberrant DNA methylation predominated in Developmental Process and Molecular Function Regulator pathways. Embryonic stem cell pathways were significantly enriched. In total, 1055 aberrantly methylated (at CpGs) and differentially expressed genes were identified, including 25 upstream regulators and sixty-one potential CpG island methylation-regulated genes. Eight methylation-regulated genes (TCF3, MYBL2, SRC, HMGA2, PPARGC1A, SLC22A1, COL2A1 and MYCN) had highly consistent gene expression patterns and prognostic value in patients with HCC, based on comparison to publicly available datasets. Conclusions: HCN-NOS has a unique, stem-cell like gene expression and DNA methylation profile related to both hepatoblastoma and HCC but distinct therefrom. Further, 8 methylation-regulated genes associated with prognosis in HCC were identified.

Randomised controlled trial comparing two group-based exercise programmes (team sport vs circuit training) on men's health: study protocol.

INTRODUCTION: Physical activity promotes physical, psychological and social health. Despite this, almost half of middle-aged (35-54 years) Australian men are insufficiently active. Exercise adherence is increased with social interaction in a group setting. Team sport can leverage the power of groups and has shown to be more intrinsically motivating than discrete exercise modes. Evaluation of the effect of team sport compared with traditional group exercise on health, particularly psychological and social health, and physical activity levels of middle-aged men is limited. This study aims to compare the effects of team sport participation and group circuit training on physical activity levels and health in insufficiently active middle-aged men. METHODS AND ANALYSIS: In this parallel randomised controlled trial, n=128 men aged 35-54 years will complete a 12-week team sport or group circuit exercise programme. Participants must self-report to not be meeting Australian physical activity guidelines or participating in team sport before recruitment. Health-related quality of life, exercise motivation, psychological needs satisfaction, sleep and physical activity levels (accelerometry), blood lipids, glucose and metabolic syndrome risk score will be assessed at baseline, end of the programme and 12 weeks follow-up. Linear mixed effect models will be used. ETHICS AND DISSEMINATION: The study has received ethical approval from the University of South Australia's Human Research Ethics Committee (Ethics Protocol 203274). Study results will be disseminated via publication in disciplinary-specific journals, conference presentations, and as part of a Doctoral thesis. TRIAL REGISTRATION NUMBER: ANCTRN12621000483853.

Effect of a 12-Week Almond-Enriched Diet on Biomarkers of Cognitive Performance, Mood, and Cardiometabolic Health in Older Overweight Adults.

Long term nut consumption is associated with reduced risk of coronary heart disease and better cognitive function. This study examined supplementing habitual diets with almonds or carbohydrate-rich snack foods (providing 15% energy) on biomarkers of cardiovascular and metabolic health, mood and cognitive performance. Participants (overweight/obese, 50-80 years) were randomised to an almond-enriched diet (AED) or isocaloric nut-free diet (NFD) for 12 weeks. Body weight, blood lipids, glucose, insulin, blood pressure (BP), arterial stiffness, cell adhesions molecules, C reactive protein (CRP), mood, and cognitive performance (working memory primary outcome), dietary profiles and energy intake/expenditure were measured at baseline and Week 12 in 128 participants (n = 63 AED, n = 65 NFD). Compared with NFD, AED was associated with altered macro and micronutrient profiles, but no differences in energy intake or expenditure. The AED significantly reduced triglycerides and SBP but there were no other changes in cardiometabolic biomarkers, mood, or cognitive performance. The inclusion of almonds in the diet improves aspects of cardiometabolic health without affecting cognitive performance or mood in overweight/obese adults.

Comparison of two low-fat diets, differing in protein and carbohydrate, on psychological wellbeing in adults with obesity and type 2 diabetes: a randomised clinical trial.

BACKGROUND: Although higher-protein diets (HP) can assist with weight loss and glycemic control, their effect on psychological wellbeing has not been established. The objective of this study was to compare the effects of a HP and a higher-carbohydrate diet (HC), combined with regular exercise, on psychological wellbeing both during weight loss (WL) and weight maintenance phases (WM). METHODS: In a parallel RCT, 61 adults with T2D (mean ± SD: BMI 34.3 ± 5.1 kg/m2, aged 55 ± 8 years) consumed a HP diet (29% protein, 34% carbohydrate, 31% fat) or an isocaloric HC diet (21%:48%:24%), with moderate intensity exercise, for 12 weeks of WL and 12 weeks of WM. Secondary data evaluating psychological wellbeing was assessed using: Problems Areas in Diabetes (PAID); Diabetes-39 Quality of Life (D-39); Short Form Health Survey (SF-36); Perceived Stress Scale-10 (PSS-10) and the Leeds Sleep Evaluation Questionnaire (LSEQ) at Weeks 0, 12 and 24 and evaluated with mixed models analysis. RESULTS: Independent of diet, improvements for PAID; D-39 diabetes control; D-39 severity of diabetes; SF-36 physical functioning and SF-36 general health were found following WL (d = 0.30 to 0.69, P ≤ 0.04 for all) which remained after 12 weeks of WM. SF-36 vitality improved more in the HP group (group x time interaction P = 0.03). Associations were seen between HbA1c and D-39 severity of diabetes rating (r = 0.30, P = 0.01) and SF-36 mental health (r = - 0.32, P = 0.003) and between weight loss and PAID (r = 0.30, P = 0.01). CONCLUSION: Several improvements in diabetes-related and general psychological wellbeing were seen similarly for both diets following weight loss and a reduction in HbA1c with most of these improvements remaining when weight loss was sustained for 12 weeks. A HP diet may provide additional increases in vitality. TRIAL REGISTRATION: The trial was prospectively registered with the Australian New Zealand Clinical Trials Registry (ACTRN 12613000008729 ) on 4 January 2013.

A randomised trial comparing low-fat diets differing in carbohydrate and protein ratio, combined with regular moderate intensity exercise, on glycaemic control, cardiometabolic risk factors, food cravings, cognitive function and psychological wellbeing in adults with type 2 diabetes: Study protocol.

BACKGROUND: Hypocaloric low-fat diets, high in protein with moderate carbohydrate (HP) can enhance weight loss, improve glycaemic control and improve cardiometabolic health risk factors in type 2 diabetes mellitus (T2DM). However, it is unclear whether the metabolic benefits observed during weight loss are sustained during energy-balance and weight maintenance. Furthermore, there is a lack of evidence regarding the effect of HP diets on food cravings, cognitive function and psychological wellbeing in T2DM, despite carbohydrate food cravings, cognitive impairment and depression being associated with hyperglycaemia. METHODS/DESIGN: Overweight/obese adults with T2DM were randomised to consume either a HP diet (n=32, ~32% protein, 33% carbohydrate, 30% fat) or a higher-carbohydrate diet (HC, n=29, ~22% protein, 51% carbohydrate, 22% fat) for 24 weeks with 30 min of moderate intensity exercise five days/week for the study duration. There were 2 phases: a 12 week weight loss phase followed by a 12 week weight maintenance phase. Primary outcome was glycaemic control (glycosylated haemoglobin; HbA1c). Secondary outcomes were cardiometabolic risk factors (body composition, fasting blood pressure, blood lipids, glucose, insulin and C-reactive protein), food cravings, cognitive function (memory; psychomotor and executive function and psychological well-being. Outcomes were measured at baseline and the end of each 12-week intervention phase. Data will be analysed as intention-to-treat using linear mixed effects models. CONCLUSION: This study will examine the effects of two dietary interventions on health outcomes in T2DM during weight loss and notably following weight maintenance where there is a paucity of evidence.

Are the dietary guidelines for meat, fat, fruit and vegetable consumption appropriate for environmental sustainability? A review of the literature.

This paper reviews the current literature around the environmental impacts of dietary recommendations. The focus of the review is on collating evidence relating to environmental impacts of the dietary advice found in the World Health Organisation guidelines, and environmental impact literature: reducing the consumption of fat, reducing the consumption of meat-based protein and animal-based foods, and increasing the consumption of fruit and vegetables. The environmental impact of reducing dietary fat intake is unclear, although reducing consumption of the food category of edible fats and oils appears to have little impact. However most, but not all, studies support environmental benefits of a reduced consumption of animal-based foods and increased consumption of fruit and vegetables. In general, it appears that adhering to dietary guidelines reduces impact on the environment, but further study is required to examine the environmental impacts of animal-based foods, and fruit and vegetable intake in depth.

Oral bovine colostrum supplementation enhances buffer capacity but not rowing performance in elite female rowers.

A randomized, double-blind, placebo controlled design was used in which 13 elite female rowers, all of whom had competed at World Championships, were supplemented with 60 g day-1 of either bovine colostrum (BC; n = 6) or concentrated whey protein powder (WP; n = 7) during 9 weeks of pre-competition training. All subjects undertook the study as a group and completed the same training program. Prior to, and after 9 weeks of supplementation and training, subjects completed an incremental rowing test (ROW1) on a rowing ergometer consisting of 3 3 4-min submaximal workloads and a 4-min maximal effort (4 max), each separated by a 1-min recovery period. The rowing test was repeated after a 15-min period of passive recovery (ROW2). The 4 max for ROW1 provided a measure of performance, and the difference between the 4 max efforts of ROW1 and ROW2 provided an index of recovery. Blood lactate concentrations and pH measured prior to exercise and at the end of each workload were used to estimate blood buffer capacity (beta). Food intake was recorded daily for dietary analysis. There were no differences in macronutrient intakes (p >.56) or training volumes (p >.99) between BC and WP during the study period. Rowing performance (distance rowed and work done) during 4 max of ROW2 was less than ROW1 at baseline (p <.05) but not different between groups (p >.05). Performance increased in both rows by Week 9 (p <.001), with no difference between groups (p >.75). However, the increase was greatest in ROW2 (p <.05), such that by Week 9 there was no longer a difference in performance between the two rows in either group (p >.05). b was not different between groups for ROW1 at baseline (BC 38.3 5.0, WP 38.2 7.2 slykes; p >.05) but was higher in BC by Week 9 (BC 40.8 5.9, WP 33.4 5.3 slykes; p <.05). b for ROW2 followed the same pattern of change as for ROW1. We conclude that supplementation with BC improves b, but not performance, in elite female rowers. It was not possible to determine whether b had any effect on recovery.